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Title: Abscisic acid ameliorates the systemic sclerosis fibroblast phenotype in vitro

Abstract

Highlights: Black-Right-Pointing-Pointer ABA is an endogenous hormone in humans, regulating different cell responses. Black-Right-Pointing-Pointer ABA reverts some of the functions altered in SSc fibroblasts to a normal phenotype. Black-Right-Pointing-Pointer UV-B irradiation increases ABA content in SSc cultures. Black-Right-Pointing-Pointer SSc fibroblasts could benefit from exposure to ABA and/or to UV-B. -- Abstract: The phytohormone abscisic acid (ABA) has been recently identified as an endogenous hormone in humans, regulating different cell functions, including inflammatory processes, insulin release and glucose uptake. Systemic sclerosis (SSc) is a chronic inflammatory disease resulting in fibrosis of skin and internal organs. In this study, we investigated the effect of exogenous ABA on fibroblasts obtained from healthy subjects and from SSc patients. Migration of control fibroblasts induced by ABA was comparable to that induced by transforming growth factor-{beta} (TGF-{beta}). Conversely, migration toward ABA, but not toward TGF-{beta}, was impaired in SSc fibroblasts. In addition, ABA increased cell proliferation in fibroblasts from SSc patients, but not from healthy subjects. Most importantly, presence of ABA significantly decreased collagen deposition by SSc fibroblasts, at the same time increasing matrix metalloproteinase-1 activity and decreasing the expression level of tissue inhibitor of metalloproteinase (TIMP-1). Thus, exogenously added ABA appeared to revert some ofmore » the functions altered in SSc fibroblasts to a normal phenotype. Interestingly, ABA levels in plasma from SSc patients were found to be significantly lower than in healthy subjects. UV-B irradiation induced an almost 3-fold increase in ABA content in SSc cultures. Altogether, these results suggest that the fibrotic skin lesions in SSc patients could benefit from exposure to high(er) ABA levels.« less

Authors:
 [1];  [2];  [2];  [3];  [1];  [3];  [1];  [2];  [1]; ;  [1];  [2]; ; ;  [3];  [3];  [2];
  1. Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV 1, 16132 Genova (Italy)
  2. (Italy)
  3. Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova (Italy)
Publication Date:
OSTI Identifier:
22207865
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 422; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSCISIC ACID; ANIMAL TISSUES; CALVES; CELL PROLIFERATION; COLLAGEN; ESTERS; FIBROBLASTS; FIBROSIS; GLUCOSE; GROWTH FACTORS; IN VITRO; INFLAMMATION; INSULIN; IRRADIATION; LIGASES; PATIENTS; PHENOTYPE; PHOSPHATES; SKIN

Citation Formats

Bruzzone, Santina, E-mail: santina.bruzzone@unige.it, Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Battaglia, Florinda, Mannino, Elena, Parodi, Alessia, Fruscione, Floriana, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Basile, Giovanna, Salis, Annalisa, Sturla, Laura, Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova, Negrini, Simone, Kalli, Francesca, Stringara, Silvia, Filaci, Gilberto, Department of Internal Medicine, Viale Benedetto XV 6, 16132 Genova, and and others. Abscisic acid ameliorates the systemic sclerosis fibroblast phenotype in vitro. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.04.107.
Bruzzone, Santina, E-mail: santina.bruzzone@unige.it, Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Battaglia, Florinda, Mannino, Elena, Parodi, Alessia, Fruscione, Floriana, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Basile, Giovanna, Salis, Annalisa, Sturla, Laura, Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova, Negrini, Simone, Kalli, Francesca, Stringara, Silvia, Filaci, Gilberto, Department of Internal Medicine, Viale Benedetto XV 6, 16132 Genova, & and others. Abscisic acid ameliorates the systemic sclerosis fibroblast phenotype in vitro. United States. doi:10.1016/J.BBRC.2012.04.107.
Bruzzone, Santina, E-mail: santina.bruzzone@unige.it, Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Battaglia, Florinda, Mannino, Elena, Parodi, Alessia, Fruscione, Floriana, Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova, Basile, Giovanna, Salis, Annalisa, Sturla, Laura, Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova, Negrini, Simone, Kalli, Francesca, Stringara, Silvia, Filaci, Gilberto, Department of Internal Medicine, Viale Benedetto XV 6, 16132 Genova, and and others. Fri . "Abscisic acid ameliorates the systemic sclerosis fibroblast phenotype in vitro". United States. doi:10.1016/J.BBRC.2012.04.107.
@article{osti_22207865,
title = {Abscisic acid ameliorates the systemic sclerosis fibroblast phenotype in vitro},
author = {Bruzzone, Santina, E-mail: santina.bruzzone@unige.it and Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova and Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova and Battaglia, Florinda and Mannino, Elena and Parodi, Alessia and Fruscione, Floriana and Advanced Biotechnology Center, Largo Rosanna Benzi 10, 16132 Genova and Basile, Giovanna and Salis, Annalisa and Sturla, Laura and Centre of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 9, 16132 Genova and Negrini, Simone and Kalli, Francesca and Stringara, Silvia and Filaci, Gilberto and Department of Internal Medicine, Viale Benedetto XV 6, 16132 Genova and and others},
abstractNote = {Highlights: Black-Right-Pointing-Pointer ABA is an endogenous hormone in humans, regulating different cell responses. Black-Right-Pointing-Pointer ABA reverts some of the functions altered in SSc fibroblasts to a normal phenotype. Black-Right-Pointing-Pointer UV-B irradiation increases ABA content in SSc cultures. Black-Right-Pointing-Pointer SSc fibroblasts could benefit from exposure to ABA and/or to UV-B. -- Abstract: The phytohormone abscisic acid (ABA) has been recently identified as an endogenous hormone in humans, regulating different cell functions, including inflammatory processes, insulin release and glucose uptake. Systemic sclerosis (SSc) is a chronic inflammatory disease resulting in fibrosis of skin and internal organs. In this study, we investigated the effect of exogenous ABA on fibroblasts obtained from healthy subjects and from SSc patients. Migration of control fibroblasts induced by ABA was comparable to that induced by transforming growth factor-{beta} (TGF-{beta}). Conversely, migration toward ABA, but not toward TGF-{beta}, was impaired in SSc fibroblasts. In addition, ABA increased cell proliferation in fibroblasts from SSc patients, but not from healthy subjects. Most importantly, presence of ABA significantly decreased collagen deposition by SSc fibroblasts, at the same time increasing matrix metalloproteinase-1 activity and decreasing the expression level of tissue inhibitor of metalloproteinase (TIMP-1). Thus, exogenously added ABA appeared to revert some of the functions altered in SSc fibroblasts to a normal phenotype. Interestingly, ABA levels in plasma from SSc patients were found to be significantly lower than in healthy subjects. UV-B irradiation induced an almost 3-fold increase in ABA content in SSc cultures. Altogether, these results suggest that the fibrotic skin lesions in SSc patients could benefit from exposure to high(er) ABA levels.},
doi = {10.1016/J.BBRC.2012.04.107},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 422,
place = {United States},
year = {Fri May 25 00:00:00 EDT 2012},
month = {Fri May 25 00:00:00 EDT 2012}
}