Intracellular trafficking of superparamagnetic iron oxide nanoparticles conjugated with TAT peptide: 3-dimensional electron tomography analysis

doi:https://doi.org/10.1016/J.BBRC.2012.04.080

Title: Intracellular trafficking of superparamagnetic iron oxide nanoparticles conjugated with TAT peptide: 3-dimensional electron tomography analysis

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Abstract

Highlights: Black-Right-Pointing-Pointer We study the intracellular localisation of TAT-SPIONs using 3-D electron tomography. Black-Right-Pointing-Pointer 3-D images of TAT-SPIONs in a cell are clearly shown. Black-Right-Pointing-Pointer Release of TAT-SPIONs from endocytic vesicles into the cytoplasm is clearly shown. -- Abstract: Internalisation of nanoparticles conjugated with cell penetrating peptides is a promising approach to various drug delivery applications. Cell penetrating peptides such as transactivating transcriptional activator (TAT) peptides derived from HIV-1 proteins are effective intracellular delivery vectors for a wide range of nanoparticles and pharmaceutical agents thanks to their amicable ability to enter cells and minimum cytotoxicity. Although different mechanisms of intracellular uptake and localisation have been proposed for TAT conjugated nanoparticles, it is necessary to visualise the particles on a 3-D plane in order to investigate the actual intracellular uptake and localisation. Here, we study the intracellular localisation and trafficking of TAT peptide conjugated superparamagnetic ion oxide nanoparticles (TAT-SPIONs) using 3-D electron tomography. 3-D tomograms clearly show the location of TAT-SPIONs in a cell and their slow release from the endocytic vesicles into the cytoplasm. The present methodology may well be utilised for further investigations of the behaviours of nanoparticles in cells and eventually for the development of nano drug deliverymore » systems.« less

Authors:

Nair, Baiju G.; Fukuda, Takahiro; Mizuki, Toru; Hanajiri, Tatsuro ^[1]; Maekawa, Toru ^[1]

Bio-Nano Electronics Research Centre, Toyo University, Saitama 350-8585 (Japan)

Publication Date:: 2012-05-18

OSTI Identifier:: 22207857

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 421; Journal Issue: 4; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; AIDS VIRUS; CYTOPLASM; DRUGS; IMAGES; IRON OXIDES; NANOSTRUCTURES; PEPTIDES; SUPERPARAMAGNETISM; TOMOGRAPHY; TOXICITY; TRANSMISSION ELECTRON MICROSCOPY

Citation Formats


                    Nair, Baiju G., Fukuda, Takahiro, Mizuki, Toru, Hanajiri, Tatsuro, and Maekawa, Toru. Intracellular trafficking of superparamagnetic iron oxide nanoparticles conjugated with TAT peptide: 3-dimensional electron tomography analysis.  United States: N. p., 2012. 
        Web.  doi:10.1016/J.BBRC.2012.04.080.

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                    Nair, Baiju G., Fukuda, Takahiro, Mizuki, Toru, Hanajiri, Tatsuro, & Maekawa, Toru. Intracellular trafficking of superparamagnetic iron oxide nanoparticles conjugated with TAT peptide: 3-dimensional electron tomography analysis.  United States.  https://doi.org/10.1016/J.BBRC.2012.04.080

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                    Nair, Baiju G., Fukuda, Takahiro, Mizuki, Toru, Hanajiri, Tatsuro, and Maekawa, Toru. Fri .  
        "Intracellular trafficking of superparamagnetic iron oxide nanoparticles conjugated with TAT peptide: 3-dimensional electron tomography analysis".  United States.  https://doi.org/10.1016/J.BBRC.2012.04.080.

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@article{osti_22207857,

  title        = {Intracellular trafficking of superparamagnetic iron oxide nanoparticles conjugated with TAT peptide: 3-dimensional electron tomography analysis},

  author       = {Nair, Baiju G. and Fukuda, Takahiro and Mizuki, Toru and Hanajiri, Tatsuro and Maekawa, Toru},

  abstractNote = {Highlights: Black-Right-Pointing-Pointer We study the intracellular localisation of TAT-SPIONs using 3-D electron tomography. Black-Right-Pointing-Pointer 3-D images of TAT-SPIONs in a cell are clearly shown. Black-Right-Pointing-Pointer Release of TAT-SPIONs from endocytic vesicles into the cytoplasm is clearly shown. -- Abstract: Internalisation of nanoparticles conjugated with cell penetrating peptides is a promising approach to various drug delivery applications. Cell penetrating peptides such as transactivating transcriptional activator (TAT) peptides derived from HIV-1 proteins are effective intracellular delivery vectors for a wide range of nanoparticles and pharmaceutical agents thanks to their amicable ability to enter cells and minimum cytotoxicity. Although different mechanisms of intracellular uptake and localisation have been proposed for TAT conjugated nanoparticles, it is necessary to visualise the particles on a 3-D plane in order to investigate the actual intracellular uptake and localisation. Here, we study the intracellular localisation and trafficking of TAT peptide conjugated superparamagnetic ion oxide nanoparticles (TAT-SPIONs) using 3-D electron tomography. 3-D tomograms clearly show the location of TAT-SPIONs in a cell and their slow release from the endocytic vesicles into the cytoplasm. The present methodology may well be utilised for further investigations of the behaviours of nanoparticles in cells and eventually for the development of nano drug delivery systems.},

  doi          = {10.1016/J.BBRC.2012.04.080},

  url          = {https://www.osti.gov/biblio/22207857},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 4,

  volume       = 421,

  place        = {United States},

  year         = {2012},

  month        = {5}

}

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https://doi.org/10.1016/J.BBRC.2012.04.080

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