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Title: Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis

Abstract

Highlights: Black-Right-Pointing-Pointer We examine how radiation affects the expression level and signal pathway of collagen. Black-Right-Pointing-Pointer TGF-{beta}1 mRNA is elevated earlier than those of collagen genes after irradiation. Black-Right-Pointing-Pointer Smad pathway mediates the expression of collagen in radiation induced fibrosis. Black-Right-Pointing-Pointer MAPK pathways are not affected in the expression of collagen after irradiation. -- Abstract: Radiation induced fibrosis occurs following a therapeutic or accidental radiation exposure in normal tissues. Tissue fibrosis is the excessive accumulation of collagen and other extracellular matrix components. This study investigated how ionizing radiation affects the expression level and signal pathway of type I collagen. Real time RT-RCR showed that both {alpha}1and {alpha}2 chain of type I collagen mRNA were elevated from 48 h after irradiation with 10 Gy in NIH3T3 cells. The relative luciferase activities of both genes and type I collagen marker were elevated at 72 h. TGF-{beta}1 mRNA was elevated earlier than those of type I collagen genes. A Western blot analysis showed the elevation of Smad phosphorylation at 72 h. Conversely, treatment with TGF-{beta} receptor inhibitor inhibited the mRNA and relative luciferase activity of type I collagen. The phosphorylation of Smad was repressed with the inhibitor, and the luciferase activity was cancelledmore » using a mutant construct of Smad binding site of {alpha}2(I) collagen gene. However, the MAPK pathways, p38, ERK1/2 and JNK, were not affected with specific inhibitors or siRNA. The data showed that the Smad pathway mediated the expression of type I collagen in radiation induced fibrosis.« less

Authors:
 [1];  [2]; ;  [3]; ;  [1];  [1]
  1. Department of Matrix Medicine, Oita University, 1-1 Idaigaoka Hasama-machi, Yufu, Oita 879-5593 (Japan)
  2. (Japan)
  3. Cell Biology, Faculty of Medicine, Oita University, 1-1 Idaigaoka Hasama-machi, Yufu, Oita 879-5593 (Japan)
Publication Date:
OSTI Identifier:
22207705
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 418; Journal Issue: 3; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; COLLAGEN; ENZYME IMMUNOASSAY; FIBROSIS; GENES; IRRADIATION; LUCIFERASE; MESSENGER-RNA; PHOSPHORYLATION; RECEPTORS

Citation Formats

Yano, Hiroyuki, Division of Radioisotope Research, Department of Research Support, Research Promotion Project, Oita University, 1-1 Idaigaoka Hasama-machi, Yufu, Oita 879-5593, Hamanaka, Ryoji, Nakamura, Miki, Sumiyoshi, Hideaki, Matsuo, Noritaka, and Yoshioka, Hidekatsu, E-mail: hidey@oita-u.ac.jp. Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.01.039.
Yano, Hiroyuki, Division of Radioisotope Research, Department of Research Support, Research Promotion Project, Oita University, 1-1 Idaigaoka Hasama-machi, Yufu, Oita 879-5593, Hamanaka, Ryoji, Nakamura, Miki, Sumiyoshi, Hideaki, Matsuo, Noritaka, & Yoshioka, Hidekatsu, E-mail: hidey@oita-u.ac.jp. Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis. United States. doi:10.1016/J.BBRC.2012.01.039.
Yano, Hiroyuki, Division of Radioisotope Research, Department of Research Support, Research Promotion Project, Oita University, 1-1 Idaigaoka Hasama-machi, Yufu, Oita 879-5593, Hamanaka, Ryoji, Nakamura, Miki, Sumiyoshi, Hideaki, Matsuo, Noritaka, and Yoshioka, Hidekatsu, E-mail: hidey@oita-u.ac.jp. Fri . "Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis". United States. doi:10.1016/J.BBRC.2012.01.039.
@article{osti_22207705,
title = {Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis},
author = {Yano, Hiroyuki and Division of Radioisotope Research, Department of Research Support, Research Promotion Project, Oita University, 1-1 Idaigaoka Hasama-machi, Yufu, Oita 879-5593 and Hamanaka, Ryoji and Nakamura, Miki and Sumiyoshi, Hideaki and Matsuo, Noritaka and Yoshioka, Hidekatsu, E-mail: hidey@oita-u.ac.jp},
abstractNote = {Highlights: Black-Right-Pointing-Pointer We examine how radiation affects the expression level and signal pathway of collagen. Black-Right-Pointing-Pointer TGF-{beta}1 mRNA is elevated earlier than those of collagen genes after irradiation. Black-Right-Pointing-Pointer Smad pathway mediates the expression of collagen in radiation induced fibrosis. Black-Right-Pointing-Pointer MAPK pathways are not affected in the expression of collagen after irradiation. -- Abstract: Radiation induced fibrosis occurs following a therapeutic or accidental radiation exposure in normal tissues. Tissue fibrosis is the excessive accumulation of collagen and other extracellular matrix components. This study investigated how ionizing radiation affects the expression level and signal pathway of type I collagen. Real time RT-RCR showed that both {alpha}1and {alpha}2 chain of type I collagen mRNA were elevated from 48 h after irradiation with 10 Gy in NIH3T3 cells. The relative luciferase activities of both genes and type I collagen marker were elevated at 72 h. TGF-{beta}1 mRNA was elevated earlier than those of type I collagen genes. A Western blot analysis showed the elevation of Smad phosphorylation at 72 h. Conversely, treatment with TGF-{beta} receptor inhibitor inhibited the mRNA and relative luciferase activity of type I collagen. The phosphorylation of Smad was repressed with the inhibitor, and the luciferase activity was cancelled using a mutant construct of Smad binding site of {alpha}2(I) collagen gene. However, the MAPK pathways, p38, ERK1/2 and JNK, were not affected with specific inhibitors or siRNA. The data showed that the Smad pathway mediated the expression of type I collagen in radiation induced fibrosis.},
doi = {10.1016/J.BBRC.2012.01.039},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 418,
place = {United States},
year = {2012},
month = {2}
}