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Title: Anti-inflammatory effects of Tat-Annexin protein on ovalbumin-induced airway inflammation in a mouse model of asthma

Abstract

Highlights: Black-Right-Pointing-Pointer We construct a cell permeable Tat-ANX1 fusion protein. Black-Right-Pointing-Pointer We examined the protective effects of Tat-ANX1 protein on OVA-induced asthma in animal models. Black-Right-Pointing-Pointer Transduced Tat-ANX1 protein protects from the OVA-induced production of cytokines and eosinophils in BAL fluid. Black-Right-Pointing-Pointer Tat-ANX1 protein markedly reduced OVA-induced MAPK in lung tissues. Black-Right-Pointing-Pointer Tat-ANX1 protein could be useful as a therapeutic agent for lung disorders including asthma. -- Abstract: Chronic airway inflammation is a key feature of bronchial asthma. Annexin-1 (ANX1) is an anti-inflammatory protein that is an important modulator and plays a key role in inflammation. Although the precise action of ANX1 remains unclear, it has emerged as a potential drug target for inflammatory diseases such as asthma. To examine the protective effects of ANX1 protein on ovalbumin (OVA)-induced asthma in animal models, we used a cell-permeable Tat-ANX1 protein. Mice sensitized and challenged with OVA antigen had an increased amount of cytokines and eosinophils in their bronchoalveolar lavage (BAL) fluid. However, administration of Tat-ANX1 protein before OVA challenge significantly decreased the levels of cytokines (interleukin (IL)-4, IL-5, and IL-13) and BAL fluid in lung tissues. Furthermore, OVA significantly increased the activation of mitogen-activated protein kinase (MAPK) in lung tissues, whereasmore » Tat-ANX1 protein markedly reduced phosphorylation of MAPKs such as extracellular signal-regulated protein kinase, p38, and stress-activated protein kinase/c-Jun N-terminal kinase. These results suggest that transduced Tat-ANX1 protein may be a potential protein therapeutic agent for the treatment of lung disorders including asthma.« less

Authors:
; ; ; ; ;  [1];  [2];  [3];  [4];  [5];  [1]
  1. Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of)
  2. Department of Life Science, Pohang University of Science and Technology, Pohang 790-784 (Korea, Republic of)
  3. Department of Biochemistry and Molecular Biology, University of Ulsan, College of Medicine, Seoul 138-736 (Korea, Republic of)
  4. Department of Biological Science, Sookmyung Women's University, Seoul 140-742 (Korea, Republic of)
  5. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22207661
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 417; Journal Issue: 3; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; ANTIGENS; ASTHMA; DRUGS; EOSINOPHILS; FLUIDS; INFLAMMATION; LAVAGE; LUNGS; LYMPHOKINES; MICE; OVA; OVALBUMIN; PHOSPHORYLATION; THERAPY

Citation Formats

Lee, Sun Hwa, Kim, Dae Won, Kim, Hye Ri, Woo, Su Jung, Kim, So Mi, Jo, Hyo Sang, Jeon, Seong Gyu, Cho, Sung-Woo, Park, Jong Hoon, Won, Moo Ho, Park, Jinseu, Eum, Won Sik, E-mail: wseum@hallym.ac.kr, and Choi, Soo Young, E-mail: sychoi@hallym.ac.kr. Anti-inflammatory effects of Tat-Annexin protein on ovalbumin-induced airway inflammation in a mouse model of asthma. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2011.12.084.
Lee, Sun Hwa, Kim, Dae Won, Kim, Hye Ri, Woo, Su Jung, Kim, So Mi, Jo, Hyo Sang, Jeon, Seong Gyu, Cho, Sung-Woo, Park, Jong Hoon, Won, Moo Ho, Park, Jinseu, Eum, Won Sik, E-mail: wseum@hallym.ac.kr, & Choi, Soo Young, E-mail: sychoi@hallym.ac.kr. Anti-inflammatory effects of Tat-Annexin protein on ovalbumin-induced airway inflammation in a mouse model of asthma. United States. https://doi.org/10.1016/J.BBRC.2011.12.084
Lee, Sun Hwa, Kim, Dae Won, Kim, Hye Ri, Woo, Su Jung, Kim, So Mi, Jo, Hyo Sang, Jeon, Seong Gyu, Cho, Sung-Woo, Park, Jong Hoon, Won, Moo Ho, Park, Jinseu, Eum, Won Sik, E-mail: wseum@hallym.ac.kr, and Choi, Soo Young, E-mail: sychoi@hallym.ac.kr. Fri . "Anti-inflammatory effects of Tat-Annexin protein on ovalbumin-induced airway inflammation in a mouse model of asthma". United States. https://doi.org/10.1016/J.BBRC.2011.12.084.
@article{osti_22207661,
title = {Anti-inflammatory effects of Tat-Annexin protein on ovalbumin-induced airway inflammation in a mouse model of asthma},
author = {Lee, Sun Hwa and Kim, Dae Won and Kim, Hye Ri and Woo, Su Jung and Kim, So Mi and Jo, Hyo Sang and Jeon, Seong Gyu and Cho, Sung-Woo and Park, Jong Hoon and Won, Moo Ho and Park, Jinseu and Eum, Won Sik, E-mail: wseum@hallym.ac.kr and Choi, Soo Young, E-mail: sychoi@hallym.ac.kr},
abstractNote = {Highlights: Black-Right-Pointing-Pointer We construct a cell permeable Tat-ANX1 fusion protein. Black-Right-Pointing-Pointer We examined the protective effects of Tat-ANX1 protein on OVA-induced asthma in animal models. Black-Right-Pointing-Pointer Transduced Tat-ANX1 protein protects from the OVA-induced production of cytokines and eosinophils in BAL fluid. Black-Right-Pointing-Pointer Tat-ANX1 protein markedly reduced OVA-induced MAPK in lung tissues. Black-Right-Pointing-Pointer Tat-ANX1 protein could be useful as a therapeutic agent for lung disorders including asthma. -- Abstract: Chronic airway inflammation is a key feature of bronchial asthma. Annexin-1 (ANX1) is an anti-inflammatory protein that is an important modulator and plays a key role in inflammation. Although the precise action of ANX1 remains unclear, it has emerged as a potential drug target for inflammatory diseases such as asthma. To examine the protective effects of ANX1 protein on ovalbumin (OVA)-induced asthma in animal models, we used a cell-permeable Tat-ANX1 protein. Mice sensitized and challenged with OVA antigen had an increased amount of cytokines and eosinophils in their bronchoalveolar lavage (BAL) fluid. However, administration of Tat-ANX1 protein before OVA challenge significantly decreased the levels of cytokines (interleukin (IL)-4, IL-5, and IL-13) and BAL fluid in lung tissues. Furthermore, OVA significantly increased the activation of mitogen-activated protein kinase (MAPK) in lung tissues, whereas Tat-ANX1 protein markedly reduced phosphorylation of MAPKs such as extracellular signal-regulated protein kinase, p38, and stress-activated protein kinase/c-Jun N-terminal kinase. These results suggest that transduced Tat-ANX1 protein may be a potential protein therapeutic agent for the treatment of lung disorders including asthma.},
doi = {10.1016/J.BBRC.2011.12.084},
url = {https://www.osti.gov/biblio/22207661}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 417,
place = {United States},
year = {2012},
month = {1}
}