skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Functional analysis of {alpha}1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells

Abstract

Highlights: Black-Right-Pointing-Pointer Human FUT6 is up-regulated in HCC tissues. Black-Right-Pointing-Pointer Expression of FUT6 promotes G0/G1-S transition and cell growth. Black-Right-Pointing-Pointer FUT6 confers a growth advantage in vivo. Black-Right-Pointing-Pointer FUT6 suppresses p21 expression through modulating PI3K/Akt signaling. -- Abstract: The {alpha}1,3/4-fucosyltransferases (FUT) subfamily are key enzymes in cell surface antigen synthesis during various biological processes. A novel role of FUTs in tumorigenesis has been discovered recently, however, the underlying mechanism remains largely unknown. Here, we characterized FUT6, a member of {alpha}1,3/4-FUT subfamily, in human hepatocellular carcinoma (HCC). In HCC tissues, the expression levels of FUT6 and its catalytic product SLe{sup x} were significantly up-regulated. Overexpression of FUT6 in HCC cells enhanced S-phase cell population, promoted cell growth and colony formation ability. Moreover, subcutaneously injection of FUT6-overexpressing cells in nude mice promoted cell growth in vivo. In addition, elevating FUT6 expression markedly induced intracellular Akt phosphorylation, and suppressed the expression of the cyclin-dependent kinases inhibitor p21. Bath application of the PI3K inhibitor blocked FUT6-induced Akt phosphorylation, p21 suppression and cell proliferation. Our results suggest that FUT6 plays an important role in HCC growth by regulating the PI3K/Akt signaling pathway.

Authors:
; ; ; ; ;  [1];  [1];  [1]
  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433 (China)
Publication Date:
OSTI Identifier:
22207627
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 417; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; CELL PROLIFERATION; COLONY FORMATION; HEPATOMAS; IN VIVO; INHIBITION; INOSITOL; MICE; MONOCLINIC LATTICES; PHOSPHORYLATION; PHOSPHOTRANSFERASES; SUBCUTANEOUS INJECTION

Citation Formats

Guo, Qiya, Guo, Bin, Wang, Yingming, Wu, Jun, Jiang, Wenjun, Zhao, Shenan, Qiao, Shouyi, and Wu, Yanhua. Functional analysis of {alpha}1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2011.11.106.
Guo, Qiya, Guo, Bin, Wang, Yingming, Wu, Jun, Jiang, Wenjun, Zhao, Shenan, Qiao, Shouyi, & Wu, Yanhua. Functional analysis of {alpha}1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells. United States. doi:10.1016/J.BBRC.2011.11.106.
Guo, Qiya, Guo, Bin, Wang, Yingming, Wu, Jun, Jiang, Wenjun, Zhao, Shenan, Qiao, Shouyi, and Wu, Yanhua. Fri . "Functional analysis of {alpha}1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells". United States. doi:10.1016/J.BBRC.2011.11.106.
@article{osti_22207627,
title = {Functional analysis of {alpha}1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells},
author = {Guo, Qiya and Guo, Bin and Wang, Yingming and Wu, Jun and Jiang, Wenjun and Zhao, Shenan and Qiao, Shouyi and Wu, Yanhua},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Human FUT6 is up-regulated in HCC tissues. Black-Right-Pointing-Pointer Expression of FUT6 promotes G0/G1-S transition and cell growth. Black-Right-Pointing-Pointer FUT6 confers a growth advantage in vivo. Black-Right-Pointing-Pointer FUT6 suppresses p21 expression through modulating PI3K/Akt signaling. -- Abstract: The {alpha}1,3/4-fucosyltransferases (FUT) subfamily are key enzymes in cell surface antigen synthesis during various biological processes. A novel role of FUTs in tumorigenesis has been discovered recently, however, the underlying mechanism remains largely unknown. Here, we characterized FUT6, a member of {alpha}1,3/4-FUT subfamily, in human hepatocellular carcinoma (HCC). In HCC tissues, the expression levels of FUT6 and its catalytic product SLe{sup x} were significantly up-regulated. Overexpression of FUT6 in HCC cells enhanced S-phase cell population, promoted cell growth and colony formation ability. Moreover, subcutaneously injection of FUT6-overexpressing cells in nude mice promoted cell growth in vivo. In addition, elevating FUT6 expression markedly induced intracellular Akt phosphorylation, and suppressed the expression of the cyclin-dependent kinases inhibitor p21. Bath application of the PI3K inhibitor blocked FUT6-induced Akt phosphorylation, p21 suppression and cell proliferation. Our results suggest that FUT6 plays an important role in HCC growth by regulating the PI3K/Akt signaling pathway.},
doi = {10.1016/J.BBRC.2011.11.106},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 417,
place = {United States},
year = {2012},
month = {1}
}