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Title: Frizzled-8 as a putative therapeutic target in human lung cancer

Abstract

Highlights: Black-Right-Pointing-Pointer Fzd-8 is over-expressed in human lung cancer. Black-Right-Pointing-Pointer shRNA knock-down of Fzd-8 inhibits proliferation and Wnt pathway in lung cancer cells. Black-Right-Pointing-Pointer shRNA knock-down of Fzd-8 suppresses tumor growth in vivo. Black-Right-Pointing-Pointer shRNA knock-down Fzd-8 sensitizes lung cancer cells to chemotherapy Taxotere. -- Abstract: Lung cancer is the leading cause of cancer related deaths worldwide. It is necessary to better understand the molecular mechanisms involved in lung cancer in order to develop more effective therapeutics for the treatment of this disease. Recent reports have shown that Wnt signaling pathway is important in a number of cancer types including lung cancer. However, the role of Frizzled-8 (Fzd-8), one of the Frizzled family of receptors for the Wnt ligands, in lung cancer still remains to be elucidated. Here in this study we showed that Fzd-8 was over-expressed in human lung cancer tissue samples and cell lines. To investigate the functional importance of the Fzd-8 over-expression in lung cancer, we used shRNA to knock down Fzd-8 mRNA in lung cancer cells expressing the gene. We observed that Fzd-8 shRNA inhibited cell proliferation along with decreased activity of Wnt pathway in vitro, and also significantly suppressed A549 xenograft model in vivo (pmore » < 0.05). Furthermore, we found that knocking down Fzd-8 by shRNA sensitized the lung cancer cells to chemotherapy Taxotere. These data suggest that Fzd-8 is a putative therapeutic target for human lung cancer and over-expression of Fzd-8 may be important for aberrant Wnt activation in lung cancer.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Cancer Clinical Study Center, Wuhan University, Wuhan 430071 (China)
  2. (China)
  3. Department of Pathology, Tianjin Chest Hospital, Tianjin 300051 (China)
  4. Department of Pathology, Henan Cancer Hospital, Zhengzhou (China)
  5. Department of Thoracic Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053 (China)
Publication Date:
OSTI Identifier:
22207618
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 417; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; CHEMOTHERAPY; DEATH; GENES; IN VITRO; IN VIVO; INHIBITION; LIGANDS; LUNGS; MESSENGER-RNA; NEOPLASMS; RECEPTORS

Citation Formats

Wang, Hua-qing, Department of Medical Oncology, Tianjin Medical University Cancer Hospital, Tianjin 300060, Xu, Mei-lin, Ma, Jie, Zhang, Yi, and Xie, Cong-hua, E-mail: publication.submission@gmail.com. Frizzled-8 as a putative therapeutic target in human lung cancer. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2011.11.055.
Wang, Hua-qing, Department of Medical Oncology, Tianjin Medical University Cancer Hospital, Tianjin 300060, Xu, Mei-lin, Ma, Jie, Zhang, Yi, & Xie, Cong-hua, E-mail: publication.submission@gmail.com. Frizzled-8 as a putative therapeutic target in human lung cancer. United States. doi:10.1016/J.BBRC.2011.11.055.
Wang, Hua-qing, Department of Medical Oncology, Tianjin Medical University Cancer Hospital, Tianjin 300060, Xu, Mei-lin, Ma, Jie, Zhang, Yi, and Xie, Cong-hua, E-mail: publication.submission@gmail.com. Fri . "Frizzled-8 as a putative therapeutic target in human lung cancer". United States. doi:10.1016/J.BBRC.2011.11.055.
@article{osti_22207618,
title = {Frizzled-8 as a putative therapeutic target in human lung cancer},
author = {Wang, Hua-qing and Department of Medical Oncology, Tianjin Medical University Cancer Hospital, Tianjin 300060 and Xu, Mei-lin and Ma, Jie and Zhang, Yi and Xie, Cong-hua, E-mail: publication.submission@gmail.com},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Fzd-8 is over-expressed in human lung cancer. Black-Right-Pointing-Pointer shRNA knock-down of Fzd-8 inhibits proliferation and Wnt pathway in lung cancer cells. Black-Right-Pointing-Pointer shRNA knock-down of Fzd-8 suppresses tumor growth in vivo. Black-Right-Pointing-Pointer shRNA knock-down Fzd-8 sensitizes lung cancer cells to chemotherapy Taxotere. -- Abstract: Lung cancer is the leading cause of cancer related deaths worldwide. It is necessary to better understand the molecular mechanisms involved in lung cancer in order to develop more effective therapeutics for the treatment of this disease. Recent reports have shown that Wnt signaling pathway is important in a number of cancer types including lung cancer. However, the role of Frizzled-8 (Fzd-8), one of the Frizzled family of receptors for the Wnt ligands, in lung cancer still remains to be elucidated. Here in this study we showed that Fzd-8 was over-expressed in human lung cancer tissue samples and cell lines. To investigate the functional importance of the Fzd-8 over-expression in lung cancer, we used shRNA to knock down Fzd-8 mRNA in lung cancer cells expressing the gene. We observed that Fzd-8 shRNA inhibited cell proliferation along with decreased activity of Wnt pathway in vitro, and also significantly suppressed A549 xenograft model in vivo (p < 0.05). Furthermore, we found that knocking down Fzd-8 by shRNA sensitized the lung cancer cells to chemotherapy Taxotere. These data suggest that Fzd-8 is a putative therapeutic target for human lung cancer and over-expression of Fzd-8 may be important for aberrant Wnt activation in lung cancer.},
doi = {10.1016/J.BBRC.2011.11.055},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 417,
place = {United States},
year = {Fri Jan 06 00:00:00 EST 2012},
month = {Fri Jan 06 00:00:00 EST 2012}
}