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Title: Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes

Abstract

Highlights: Black-Right-Pointing-Pointer Lipin-1 affects lipid metabolism, adipocyte differentiation, and transcription. Black-Right-Pointing-Pointer Adipose lipin-1 expression is reduced in obesity. Black-Right-Pointing-Pointer Lipin-1 depletion using siRNA in 3T3-L1 adipocytes increased MCP-1 expression. Black-Right-Pointing-Pointer Lipin-1 is involved in adipose inflammation. -- Abstract: Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-{kappa}B activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.

Authors:
 [1];  [2];  [3]; ;  [1]; ; ;  [4];  [5]; ;  [6]; ;  [7];  [1]
  1. Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
  2. (Japan)
  3. Innovation Center, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065 (Japan)
  4. Department of Fixed Prosthodontics and Oral Implantology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
  5. Department of Dental Anesthesiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
  6. Department of Clinical Laboratory, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
  7. Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)
Publication Date:
OSTI Identifier:
22207568
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 415; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL DIFFERENTIATION; GENES; INFLAMMATION; INHIBITION; INSULIN; LIPIDS; MESSENGER-RNA; METABOLIC DISEASES; METABOLISM; MONOCYTES; TRANSCRIPTION

Citation Formats

Takahashi, Nobuhiko, E-mail: ntkhs@hoku-iryo-u.ac.jp, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, and Ieko, Masahiro. Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.10.060.
Takahashi, Nobuhiko, E-mail: ntkhs@hoku-iryo-u.ac.jp, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, & Ieko, Masahiro. Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes. United States. doi:10.1016/J.BBRC.2011.10.060.
Takahashi, Nobuhiko, E-mail: ntkhs@hoku-iryo-u.ac.jp, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, and Ieko, Masahiro. Fri . "Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes". United States. doi:10.1016/J.BBRC.2011.10.060.
@article{osti_22207568,
title = {Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes},
author = {Takahashi, Nobuhiko, E-mail: ntkhs@hoku-iryo-u.ac.jp and Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 and Yoshizaki, Takayuki and Hiranaka, Natsumi and Suzuki, Takeshi and Yui, Tomoo and Akanuma, Masayasu and Oka, Kazuya and Kanazawa, Kaoru and Yoshida, Mika and Naito, Sumiyoshi and Fujiya, Mikihiro and Kohgo, Yutaka and Ieko, Masahiro},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Lipin-1 affects lipid metabolism, adipocyte differentiation, and transcription. Black-Right-Pointing-Pointer Adipose lipin-1 expression is reduced in obesity. Black-Right-Pointing-Pointer Lipin-1 depletion using siRNA in 3T3-L1 adipocytes increased MCP-1 expression. Black-Right-Pointing-Pointer Lipin-1 is involved in adipose inflammation. -- Abstract: Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-{kappa}B activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.},
doi = {10.1016/J.BBRC.2011.10.060},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 415,
place = {United States},
year = {Fri Nov 11 00:00:00 EST 2011},
month = {Fri Nov 11 00:00:00 EST 2011}
}