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Title: Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth

Abstract

Highlights: Black-Right-Pointing-Pointer MSC like cells were derived from hESC by a simple and reproducible method. Black-Right-Pointing-Pointer Differentiation and immunosuppressive features of MSCl cells were similar to bmMSC. Black-Right-Pointing-Pointer MSCl cells as feeder cells support the undifferentiated growth of hESC. -- Abstract: Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.

Authors:
 [1]; ;  [2]; ; ;  [1];  [1]
  1. Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest (Hungary)
  2. Department of Immunology, Medical and Health Science Centre, University of Debrecen, Debrecen (Hungary)
Publication Date:
OSTI Identifier:
22207535
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 414; Journal Issue: 3; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW; CELL PROLIFERATION; FLUORESCENCE; INHIBITION; LYMPHOCYTES; PROTEINS; STEM CELLS

Citation Formats

Varga, Nora, Vereb, Zoltan, Rajnavoelgyi, Eva, Nemet, Katalin, Uher, Ferenc, Sarkadi, Balazs, and Apati, Agota, E-mail: apati@kkk.org.hu. Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.09.089.
Varga, Nora, Vereb, Zoltan, Rajnavoelgyi, Eva, Nemet, Katalin, Uher, Ferenc, Sarkadi, Balazs, & Apati, Agota, E-mail: apati@kkk.org.hu. Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth. United States. doi:10.1016/J.BBRC.2011.09.089.
Varga, Nora, Vereb, Zoltan, Rajnavoelgyi, Eva, Nemet, Katalin, Uher, Ferenc, Sarkadi, Balazs, and Apati, Agota, E-mail: apati@kkk.org.hu. Fri . "Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth". United States. doi:10.1016/J.BBRC.2011.09.089.
@article{osti_22207535,
title = {Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth},
author = {Varga, Nora and Vereb, Zoltan and Rajnavoelgyi, Eva and Nemet, Katalin and Uher, Ferenc and Sarkadi, Balazs and Apati, Agota, E-mail: apati@kkk.org.hu},
abstractNote = {Highlights: Black-Right-Pointing-Pointer MSC like cells were derived from hESC by a simple and reproducible method. Black-Right-Pointing-Pointer Differentiation and immunosuppressive features of MSCl cells were similar to bmMSC. Black-Right-Pointing-Pointer MSCl cells as feeder cells support the undifferentiated growth of hESC. -- Abstract: Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.},
doi = {10.1016/J.BBRC.2011.09.089},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 414,
place = {United States},
year = {2011},
month = {10}
}