Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis

Wang, Yifan; Pan, Juncheng; Che, Yongzhe; Yin, Jian; Zhao, Qing

doi:10.1016/J.BBRC.2011.07.102

Title: Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis

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Abstract

Highlights: {yields} Hv1 is specifically expressed in highly metastatic human breast tumor tissues. {yields} Hv1 regulates breast cancer cytosolic pH. {yields} Hv1 acidifies extracellular milieu. {yields} Hv1 exacerbates the migratory ability of metastatic cells. -- Abstract: The newly discovered human voltage-gated proton channel Hv1 is essential for proton transfer, which contains a voltage sensor domain (VSD) without a pore domain. We report here for the first time that Hv1 is specifically expressed in the highly metastatic human breast tumor tissues, but not in poorly metastatic breast cancer tissues, detected by immunohistochemistry. Meanwhile, real-time RT-PCR and immunocytochemistry showed that the expression levels of Hv1 have significant differences among breast cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-468, MDA-MB-453, T-47D and SK-BR-3, in which Hv1 is expressed at a high level in highly metastatic human breast cancer cell line MDA-MB-231, but at a very low level in poorly metastatic human breast cancer cell line MCF-7. Inhibition of Hv1 expression in the highly metastatic MDA-MB-231 cells by small interfering RNA (siRNA) significantly decreases the invasion and migration of the cells. The intracellular pH of MDA-MB-231 cells down-regulated Hv1 expression by siRNA is obviously decreased compared with MDA-MB-231 with the scrambled siRNA. The expression of matrixmore »« less

Authors:

Wang, Yifan ^[1]; Pan, Juncheng ^[1]; Che, Yongzhe ^[2]; Yin, Jian ^[3]; Zhao, Qing ^[1]

The Key Laboratory of Bioactive Materials, Ministry of Education, School of Physics Science, Nankai University, Tianjin 300071 (China)
The Key Laboratory of Bioactive Materials, Ministry of Education, School of Medicine, Nankai University, Tianjin 300071 (China)
Cancer Institute and Hospital, Tianjin Medical University, Tianjin 300060 (China)

Publication Date:: Fri Aug 26 00:00:00 EDT 2011

OSTI Identifier:: 22207468

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 412; Journal Issue: 2; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; INHIBITION; MAMMARY GLANDS; METASTASES; NEOPLASMS; PH VALUE; POLYMERASE CHAIN REACTION; RNA; SENSORS

Citation Formats


                    Wang, Yifan, Li, Shu Jie, E-mail: shujieli@nankai.edu.cn, Pan, Juncheng, Che, Yongzhe, Yin, Jian, and Zhao, Qing. Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis.  United States: N. p., 2011. 
        Web.  doi:10.1016/J.BBRC.2011.07.102.

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                    Wang, Yifan, Li, Shu Jie, E-mail: shujieli@nankai.edu.cn, Pan, Juncheng, Che, Yongzhe, Yin, Jian, & Zhao, Qing. Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis.  United States.  https://doi.org/10.1016/J.BBRC.2011.07.102

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                    Wang, Yifan, Li, Shu Jie, E-mail: shujieli@nankai.edu.cn, Pan, Juncheng, Che, Yongzhe, Yin, Jian, and Zhao, Qing. 2011.  
        "Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis".  United States.  https://doi.org/10.1016/J.BBRC.2011.07.102.

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@article{osti_22207468,

  title        = {Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis},

  author       = {Wang, Yifan and Li, Shu Jie, E-mail: shujieli@nankai.edu.cn and Pan, Juncheng and Che, Yongzhe and Yin, Jian and Zhao, Qing},

  abstractNote = {Highlights: {yields} Hv1 is specifically expressed in highly metastatic human breast tumor tissues. {yields} Hv1 regulates breast cancer cytosolic pH. {yields} Hv1 acidifies extracellular milieu. {yields} Hv1 exacerbates the migratory ability of metastatic cells. -- Abstract: The newly discovered human voltage-gated proton channel Hv1 is essential for proton transfer, which contains a voltage sensor domain (VSD) without a pore domain. We report here for the first time that Hv1 is specifically expressed in the highly metastatic human breast tumor tissues, but not in poorly metastatic breast cancer tissues, detected by immunohistochemistry. Meanwhile, real-time RT-PCR and immunocytochemistry showed that the expression levels of Hv1 have significant differences among breast cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-468, MDA-MB-453, T-47D and SK-BR-3, in which Hv1 is expressed at a high level in highly metastatic human breast cancer cell line MDA-MB-231, but at a very low level in poorly metastatic human breast cancer cell line MCF-7. Inhibition of Hv1 expression in the highly metastatic MDA-MB-231 cells by small interfering RNA (siRNA) significantly decreases the invasion and migration of the cells. The intracellular pH of MDA-MB-231 cells down-regulated Hv1 expression by siRNA is obviously decreased compared with MDA-MB-231 with the scrambled siRNA. The expression of matrix metalloproteinase-2 and gelatinase activity in MDA-MB-231 cells suppressed Hv1 by siRNA were reduced. Our results strongly suggest that Hv1 regulates breast cancer intracellular pH and exacerbates the migratory ability of metastatic cells.},

  doi          = {10.1016/J.BBRC.2011.07.102},

  url          = {https://www.osti.gov/biblio/22207468},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 2,

  volume       = 412,

  place        = {United States},

  year         = {Fri Aug 26 00:00:00 EDT 2011},

  month        = {Fri Aug 26 00:00:00 EDT 2011}

}

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