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Title: Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-{alpha}

Abstract

Highlights: {yields} TNF-{alpha} inhibits POEM gene expression. {yields} Inhibition of POEM gene expression is caused by NF-{kappa}B activation by TNF-{alpha}. {yields} Over-expression of POEM recovers inhibition of osteoblast differentiation by TNF-{alpha}. -- Abstract: POEM, also known as nephronectin, is an extracellular matrix protein considered to be a positive regulator of osteoblast differentiation. In the present study, we found that tumor necrosis factor-{alpha} (TNF-{alpha}), a key regulator of bone matrix properties and composition that also inhibits terminal osteoblast differentiation, strongly inhibited POEM expression in the mouse osteoblastic cell line MC3T3-E1. TNF-{alpha}-induced down-regulation of POEM gene expression occurred in both time- and dose-dependent manners through the nuclear factor kappa B (NF-{kappa}B) pathway. In addition, expressions of marker genes in differentiated osteoblasts were down-regulated by TNF-{alpha} in a manner consistent with our findings for POEM, while over-expression of POEM recovered TNF-{alpha}-induced inhibition of osteoblast differentiation. These results suggest that TNF-{alpha} inhibits POEM expression through the NF-{kappa}B signaling pathway and down-regulation of POEM influences the inhibition of osteoblast differentiation by TNF-{alpha}.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3]; ; ; ;  [1];  [4];  [3];  [1]
  1. Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555 (Japan)
  2. (Japan)
  3. Department of Periodontology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta, Tokyo 145-8515 (Japan)
  4. Laboratory for Comparative Neurogenesis, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan)
Publication Date:
OSTI Identifier:
22207400
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 410; Journal Issue: 4; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CONNECTIVE TISSUE CELLS; DOSES; GENE REGULATION; GENES; INHIBITION; MICE; PROTEINS; SKELETON

Citation Formats

Tsukasaki, Masayuki, Yamada, Atsushi, E-mail: yamadaa@dent.showa-u.ac.jp, Suzuki, Dai, Aizawa, Ryo, Department of Periodontology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta, Tokyo 145-8515, Miyazono, Agasa, Miyamoto, Yoichi, Suzawa, Tetsuo, Takami, Masamichi, Yoshimura, Kentaro, Morimura, Naoko, Yamamoto, Matsuo, and Kamijo, Ryutaro. Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-{alpha}. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.06.048.
Tsukasaki, Masayuki, Yamada, Atsushi, E-mail: yamadaa@dent.showa-u.ac.jp, Suzuki, Dai, Aizawa, Ryo, Department of Periodontology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta, Tokyo 145-8515, Miyazono, Agasa, Miyamoto, Yoichi, Suzawa, Tetsuo, Takami, Masamichi, Yoshimura, Kentaro, Morimura, Naoko, Yamamoto, Matsuo, & Kamijo, Ryutaro. Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-{alpha}. United States. doi:10.1016/J.BBRC.2011.06.048.
Tsukasaki, Masayuki, Yamada, Atsushi, E-mail: yamadaa@dent.showa-u.ac.jp, Suzuki, Dai, Aizawa, Ryo, Department of Periodontology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta, Tokyo 145-8515, Miyazono, Agasa, Miyamoto, Yoichi, Suzawa, Tetsuo, Takami, Masamichi, Yoshimura, Kentaro, Morimura, Naoko, Yamamoto, Matsuo, and Kamijo, Ryutaro. Fri . "Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-{alpha}". United States. doi:10.1016/J.BBRC.2011.06.048.
@article{osti_22207400,
title = {Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-{alpha}},
author = {Tsukasaki, Masayuki and Yamada, Atsushi, E-mail: yamadaa@dent.showa-u.ac.jp and Suzuki, Dai and Aizawa, Ryo and Department of Periodontology, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ohta, Tokyo 145-8515 and Miyazono, Agasa and Miyamoto, Yoichi and Suzawa, Tetsuo and Takami, Masamichi and Yoshimura, Kentaro and Morimura, Naoko and Yamamoto, Matsuo and Kamijo, Ryutaro},
abstractNote = {Highlights: {yields} TNF-{alpha} inhibits POEM gene expression. {yields} Inhibition of POEM gene expression is caused by NF-{kappa}B activation by TNF-{alpha}. {yields} Over-expression of POEM recovers inhibition of osteoblast differentiation by TNF-{alpha}. -- Abstract: POEM, also known as nephronectin, is an extracellular matrix protein considered to be a positive regulator of osteoblast differentiation. In the present study, we found that tumor necrosis factor-{alpha} (TNF-{alpha}), a key regulator of bone matrix properties and composition that also inhibits terminal osteoblast differentiation, strongly inhibited POEM expression in the mouse osteoblastic cell line MC3T3-E1. TNF-{alpha}-induced down-regulation of POEM gene expression occurred in both time- and dose-dependent manners through the nuclear factor kappa B (NF-{kappa}B) pathway. In addition, expressions of marker genes in differentiated osteoblasts were down-regulated by TNF-{alpha} in a manner consistent with our findings for POEM, while over-expression of POEM recovered TNF-{alpha}-induced inhibition of osteoblast differentiation. These results suggest that TNF-{alpha} inhibits POEM expression through the NF-{kappa}B signaling pathway and down-regulation of POEM influences the inhibition of osteoblast differentiation by TNF-{alpha}.},
doi = {10.1016/J.BBRC.2011.06.048},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 410,
place = {United States},
year = {2011},
month = {7}
}