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Title: Transcription factor COUP-TFII is indispensable for venous and lymphatic development in zebrafish and Xenopus laevis

Abstract

Highlights: {yields} COUP-TFII deficiency in zebrafish affects arterio-venous EC specification. {yields} COUP-TFII is indispensable for lymphatic development in zebrafish. {yields} COUP-TFII knockdown in Xenopus disrupts lymphatic EC differentiation and migration. {yields} COUP-TFII's role in EC fate decisions is evolutionary conserved. -- Abstract: Transcription factors play a central role in cell fate determination. Gene targeting in mice revealed that Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII, also known as Nuclear Receptor 2F2 or NR2F2) induces a venous phenotype in endothelial cells (ECs). More recently, NR2F2 was shown to be required for initiating the expression of Prox1, responsible for lymphatic commitment of venous ECs. Small animal models like zebrafish embryos and Xenopus laevis tadpoles have been very useful to elucidate mechanisms of (lymph) vascular development. Therefore, the role of NR2F2 in (lymph) vascular development was studied by eliminating its expression in these models. Like in mice, absence of NR2F2 in zebrafish resulted in distinct vascular defects including loss of venous marker expression, major trunk vessel fusion and vascular leakage. Both in zebrafish and Xenopus the development of the main lymphatic structures was severely hampered. NR2F2 knockdown significantly decreased prox1 expression in zebrafish ECs and the same manipulation affected lymphatic (L)EC commitment, migrationmore » and function in Xenopus tadpoles. Therefore, the role of NR2F2 in EC fate determination is evolutionary conserved.« less

Authors:
 [1];  [1];  [2];  [3];  [2];  [3];  [2];  [3];  [1]
  1. Center for Molecular and Vascular Biology, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven (Belgium)
  2. Vesalius Research Center, VIB, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven (Belgium)
  3. (Belgium)
Publication Date:
OSTI Identifier:
22204970
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 410; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMPHIBIANS; ANGIOGENESIS; AORTA; BLOOD; CHICKENS; EMBRYOS; FERTILIZATION; GROWTH FACTORS; IN-SITU HYBRIDIZATION; ISOTHIOCYANATES; LARVAE; LYMPH; LYMPH VESSELS; MICE; OVALBUMIN; PHENOTYPE; PROMOTERS; RECEPTORS; TRANSCRIPTION FACTORS; VEINS

Citation Formats

Aranguren, Xabier L., E-mail: xabier.lopezaranguren@med.kuleuven.be, Beerens, Manu, E-mail: manu.beerens@med.kuleuven.be, Vandevelde, Wouter, E-mail: woutervandevelde@gmail.com, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, Dewerchin, Mieke, E-mail: mieke.dewerchin@vib-kuleuven.be, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, Carmeliet, Peter, E-mail: peter.carmeliet@vib-kuleuven.be, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, and Luttun, Aernout, E-mail: aernout.luttun@med.kuleuven.be. Transcription factor COUP-TFII is indispensable for venous and lymphatic development in zebrafish and Xenopus laevis. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.05.117.
Aranguren, Xabier L., E-mail: xabier.lopezaranguren@med.kuleuven.be, Beerens, Manu, E-mail: manu.beerens@med.kuleuven.be, Vandevelde, Wouter, E-mail: woutervandevelde@gmail.com, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, Dewerchin, Mieke, E-mail: mieke.dewerchin@vib-kuleuven.be, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, Carmeliet, Peter, E-mail: peter.carmeliet@vib-kuleuven.be, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, & Luttun, Aernout, E-mail: aernout.luttun@med.kuleuven.be. Transcription factor COUP-TFII is indispensable for venous and lymphatic development in zebrafish and Xenopus laevis. United States. doi:10.1016/J.BBRC.2011.05.117.
Aranguren, Xabier L., E-mail: xabier.lopezaranguren@med.kuleuven.be, Beerens, Manu, E-mail: manu.beerens@med.kuleuven.be, Vandevelde, Wouter, E-mail: woutervandevelde@gmail.com, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, Dewerchin, Mieke, E-mail: mieke.dewerchin@vib-kuleuven.be, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, Carmeliet, Peter, E-mail: peter.carmeliet@vib-kuleuven.be, Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven, and Luttun, Aernout, E-mail: aernout.luttun@med.kuleuven.be. Fri . "Transcription factor COUP-TFII is indispensable for venous and lymphatic development in zebrafish and Xenopus laevis". United States. doi:10.1016/J.BBRC.2011.05.117.
@article{osti_22204970,
title = {Transcription factor COUP-TFII is indispensable for venous and lymphatic development in zebrafish and Xenopus laevis},
author = {Aranguren, Xabier L., E-mail: xabier.lopezaranguren@med.kuleuven.be and Beerens, Manu, E-mail: manu.beerens@med.kuleuven.be and Vandevelde, Wouter, E-mail: woutervandevelde@gmail.com and Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven and Dewerchin, Mieke, E-mail: mieke.dewerchin@vib-kuleuven.be and Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven and Carmeliet, Peter, E-mail: peter.carmeliet@vib-kuleuven.be and Vesalius Research Center, Katholieke Universiteit Leuven, Campus Gasthuisberg, Onderwijs and Navorsing 1, Herestraat 49, B-3000 Leuven and Luttun, Aernout, E-mail: aernout.luttun@med.kuleuven.be},
abstractNote = {Highlights: {yields} COUP-TFII deficiency in zebrafish affects arterio-venous EC specification. {yields} COUP-TFII is indispensable for lymphatic development in zebrafish. {yields} COUP-TFII knockdown in Xenopus disrupts lymphatic EC differentiation and migration. {yields} COUP-TFII's role in EC fate decisions is evolutionary conserved. -- Abstract: Transcription factors play a central role in cell fate determination. Gene targeting in mice revealed that Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII, also known as Nuclear Receptor 2F2 or NR2F2) induces a venous phenotype in endothelial cells (ECs). More recently, NR2F2 was shown to be required for initiating the expression of Prox1, responsible for lymphatic commitment of venous ECs. Small animal models like zebrafish embryos and Xenopus laevis tadpoles have been very useful to elucidate mechanisms of (lymph) vascular development. Therefore, the role of NR2F2 in (lymph) vascular development was studied by eliminating its expression in these models. Like in mice, absence of NR2F2 in zebrafish resulted in distinct vascular defects including loss of venous marker expression, major trunk vessel fusion and vascular leakage. Both in zebrafish and Xenopus the development of the main lymphatic structures was severely hampered. NR2F2 knockdown significantly decreased prox1 expression in zebrafish ECs and the same manipulation affected lymphatic (L)EC commitment, migration and function in Xenopus tadpoles. Therefore, the role of NR2F2 in EC fate determination is evolutionary conserved.},
doi = {10.1016/J.BBRC.2011.05.117},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 410,
place = {United States},
year = {2011},
month = {6}
}