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Title: Testicular Sertoli cells influence the proliferation and immunogenicity of co-cultured endothelial cells

Abstract

Research highlights: {yields} The proliferation of dramatic increased by co-cultured with Sertoli cells. {yields} VEGF receptor-2 expression of ECs was up-regulated by co-cultured with Sertoli cells. {yields} The MHC expression of ECs induced by INF-{gamma} and IL-6, IL-8 and sICAM induced by TNF-{alpha} decreased respectively after co-cultured with Sertoli cells. {yields} ECs co-cultured with Sertoli cells also didn't increase the stimulation index of spleen lymphocytes. -- Abstract: The major problem of the application of endothelial cells (ECs) in transplantation is the lack of proliferation and their immunogenicity. In this study, we co-cultured ECs with Sertoli cells to monitor whether Sertoli cells can influence the proliferation and immunogenicity of co-cultured ECs. Sertoli cells were isolated from adult testicular tissue. ECs were divided into the control group and the experimental group, which included three sub-groups co-cultured with 1 x 10{sup 3}, 1 x 10{sup 4} or 1 x 10{sup 5} cell/ml of Sertoli cells. The growth and proliferation of ECs were observed microscopically, and the expression of vascular endothelial growth factor (VEGF) receptor-2 (KDR) was examined by Western blotting. In another experiment, ECs were divided into the control group, the single culture group and the co-culture group with the optimal concentration ofmore » Sertoli cells. After INF-{gamma} and TNF-{alpha} were added to the culture medium, MHC II antigen expression was detected by immunofluorescence staining and western blotting; interleukin (IL)-6, IL-8 and soluble intercellular adhesion molecule (sICAM) were measured in the culture medium by ELISA. We demonstrated that 1 x 10{sup 4} cell/ml Sertoli cells promoted the proliferation of co-cultured ECs more dramatically than that in other groups (P < 0.05). Western blotting showed that 1 x 10{sup 4} cell/ml of the Sertoli cells was most effective in the up-regulation of KDR expression in the co-cultured ECs (P < 0.05). Sertoli cells can effectively suppress INF-{gamma}-induced MHC II antigen expression in co-cultured ECs compared with single culture group (P < 0.05). TNF-{alpha} induced the expression of IL-6, IL-8 and sICAM in ECs. When co-cultured with Sertoli cells, their expressions were significantly lower than in the EC single culture group (P < 0.05). ECs co-cultured with Sertoli cells also did not significantly increase the stimulation index of spleen lymphocytes compared to the single culture group (P < 0.05). Our results suggested that co-culturing with Sertoli cells can significantly promote the proliferation of ECs, accelerate post-transplant angiogenesis, while reduce EC immunogenicity and stimulus to lymphocytes.« less

Authors:
 [1]; ; ; ; ; ;  [1]
  1. Department of Rheumatism and Immunity, The First Affiliated Hospital Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061 (China)
Publication Date:
OSTI Identifier:
22204764
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 404; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANGIOGENESIS; ANIMAL TISSUES; ANTIGENS; CELL PROLIFERATION; CONCENTRATION RATIO; CULTURE MEDIA; ENZYME IMMUNOASSAY; GENE REGULATION; GROWTH FACTORS; LYMPHOCYTES; RECEPTORS; SPLEEN; TESTES

Citation Formats

Fan, Ping, E-mail: fanpinggoodluck@163.com, He, Lan, Pu, Dan, Lv, Xiaohong, Zhou, Wenxu, Sun, Yining, and Hu, Nan. Testicular Sertoli cells influence the proliferation and immunogenicity of co-cultured endothelial cells. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2010.12.068.
Fan, Ping, E-mail: fanpinggoodluck@163.com, He, Lan, Pu, Dan, Lv, Xiaohong, Zhou, Wenxu, Sun, Yining, & Hu, Nan. Testicular Sertoli cells influence the proliferation and immunogenicity of co-cultured endothelial cells. United States. doi:10.1016/J.BBRC.2010.12.068.
Fan, Ping, E-mail: fanpinggoodluck@163.com, He, Lan, Pu, Dan, Lv, Xiaohong, Zhou, Wenxu, Sun, Yining, and Hu, Nan. Fri . "Testicular Sertoli cells influence the proliferation and immunogenicity of co-cultured endothelial cells". United States. doi:10.1016/J.BBRC.2010.12.068.
@article{osti_22204764,
title = {Testicular Sertoli cells influence the proliferation and immunogenicity of co-cultured endothelial cells},
author = {Fan, Ping, E-mail: fanpinggoodluck@163.com and He, Lan and Pu, Dan and Lv, Xiaohong and Zhou, Wenxu and Sun, Yining and Hu, Nan},
abstractNote = {Research highlights: {yields} The proliferation of dramatic increased by co-cultured with Sertoli cells. {yields} VEGF receptor-2 expression of ECs was up-regulated by co-cultured with Sertoli cells. {yields} The MHC expression of ECs induced by INF-{gamma} and IL-6, IL-8 and sICAM induced by TNF-{alpha} decreased respectively after co-cultured with Sertoli cells. {yields} ECs co-cultured with Sertoli cells also didn't increase the stimulation index of spleen lymphocytes. -- Abstract: The major problem of the application of endothelial cells (ECs) in transplantation is the lack of proliferation and their immunogenicity. In this study, we co-cultured ECs with Sertoli cells to monitor whether Sertoli cells can influence the proliferation and immunogenicity of co-cultured ECs. Sertoli cells were isolated from adult testicular tissue. ECs were divided into the control group and the experimental group, which included three sub-groups co-cultured with 1 x 10{sup 3}, 1 x 10{sup 4} or 1 x 10{sup 5} cell/ml of Sertoli cells. The growth and proliferation of ECs were observed microscopically, and the expression of vascular endothelial growth factor (VEGF) receptor-2 (KDR) was examined by Western blotting. In another experiment, ECs were divided into the control group, the single culture group and the co-culture group with the optimal concentration of Sertoli cells. After INF-{gamma} and TNF-{alpha} were added to the culture medium, MHC II antigen expression was detected by immunofluorescence staining and western blotting; interleukin (IL)-6, IL-8 and soluble intercellular adhesion molecule (sICAM) were measured in the culture medium by ELISA. We demonstrated that 1 x 10{sup 4} cell/ml Sertoli cells promoted the proliferation of co-cultured ECs more dramatically than that in other groups (P < 0.05). Western blotting showed that 1 x 10{sup 4} cell/ml of the Sertoli cells was most effective in the up-regulation of KDR expression in the co-cultured ECs (P < 0.05). Sertoli cells can effectively suppress INF-{gamma}-induced MHC II antigen expression in co-cultured ECs compared with single culture group (P < 0.05). TNF-{alpha} induced the expression of IL-6, IL-8 and sICAM in ECs. When co-cultured with Sertoli cells, their expressions were significantly lower than in the EC single culture group (P < 0.05). ECs co-cultured with Sertoli cells also did not significantly increase the stimulation index of spleen lymphocytes compared to the single culture group (P < 0.05). Our results suggested that co-culturing with Sertoli cells can significantly promote the proliferation of ECs, accelerate post-transplant angiogenesis, while reduce EC immunogenicity and stimulus to lymphocytes.},
doi = {10.1016/J.BBRC.2010.12.068},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 404,
place = {United States},
year = {Fri Jan 21 00:00:00 EST 2011},
month = {Fri Jan 21 00:00:00 EST 2011}
}