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Title: ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma

Abstract

Research highlights: {yields} Strong ADAM15 expression is found in normal melanocytes. {yields} ADAM15 expression is significantly downregulated in patients with melanoma metastasis. {yields} TGF-{beta} can downregulate ADAM15 expression in melanoma cells. {yields} Overexpression of ADAM15 in melanoma cells inhibits migration, proliferation and invasion of melanoma cells. {yields} Conclusion: ADAM15 represents an tumor suppressor protein in melanoma. -- Abstract: In a mouse melanoma metastasis model it has been recently shown that ADAM15 overexpression in melanoma cells significantly reduced the number of metastatic nodules on the lung. Unfortunately, the expression of ADAM15 in human melanoma tissue has not been determined so far. In our study, we characterized the expression of ADAM15 in tissue micro-arrays of patients with primary melanoma with melanoma metastasis. ADAM15 was expressed in melanocytes and endothelial cells of benign nevi and melanoma tissue. Importantly, ADAM15 was significantly downregulated in melanoma metastasis compared to primary melanoma. We further demonstrate that IFN-{gamma} and TGF-{beta} downregulate ADAM15 protein levels in melanoma cells. To investigate the role of ADAM15 in melanoma progression, we overexpressed ADAM15 in melanoma cells. Importantly, overexpression of ADAM15 in melanoma cells reduced the migration, invasion and the anchorage dependent and independent cell growth of melanoma cells. In summary, themore » downregulation of ADAM15 plays an important role in melanoma progression and ADAM15 act as a tumorsuppressor in melanoma.« less

Authors:
;  [1]; ; ;  [2];  [3];  [1];  [4];  [5];  [1]
  1. Pharmazentrum Frankfurt/ZAFES, University Hospital Goethe University Frankfurt, Frankfurt am Main (Germany)
  2. Department of Dermatology, Clinic of the Goethe-University, Theodor-Stern-Kai, Frankfurt (Germany)
  3. Division of Rheumatology, Goethe University, Frankfurt am Main (Germany)
  4. Department of Pathology, Institute of Surgical Pathology, University Hospital, Zurich (Switzerland)
  5. Department of Dermatology, University Hospital Zurich (Switzerland)
Publication Date:
OSTI Identifier:
22202817
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 401; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL CELLS; ANIMAL TISSUES; CELL PROLIFERATION; LUNGS; MELANIN; MELANOMAS; METASTASES; MICE; PATIENTS; PROTEINS

Citation Formats

Ungerer, Christopher, Doberstein, Kai, Buerger, Claudia, Hardt, Katja, Boehncke, Wolf-Henning, Boehm, Beate, Pfeilschifter, Josef, Dummer, Reinhard, Mihic-Probst, Daniela, and Gutwein, Paul. ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.09.055.
Ungerer, Christopher, Doberstein, Kai, Buerger, Claudia, Hardt, Katja, Boehncke, Wolf-Henning, Boehm, Beate, Pfeilschifter, Josef, Dummer, Reinhard, Mihic-Probst, Daniela, & Gutwein, Paul. ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma. United States. https://doi.org/10.1016/J.BBRC.2010.09.055
Ungerer, Christopher, Doberstein, Kai, Buerger, Claudia, Hardt, Katja, Boehncke, Wolf-Henning, Boehm, Beate, Pfeilschifter, Josef, Dummer, Reinhard, Mihic-Probst, Daniela, and Gutwein, Paul. 2010. "ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma". United States. https://doi.org/10.1016/J.BBRC.2010.09.055.
@article{osti_22202817,
title = {ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma},
author = {Ungerer, Christopher and Doberstein, Kai and Buerger, Claudia and Hardt, Katja and Boehncke, Wolf-Henning and Boehm, Beate and Pfeilschifter, Josef and Dummer, Reinhard and Mihic-Probst, Daniela and Gutwein, Paul},
abstractNote = {Research highlights: {yields} Strong ADAM15 expression is found in normal melanocytes. {yields} ADAM15 expression is significantly downregulated in patients with melanoma metastasis. {yields} TGF-{beta} can downregulate ADAM15 expression in melanoma cells. {yields} Overexpression of ADAM15 in melanoma cells inhibits migration, proliferation and invasion of melanoma cells. {yields} Conclusion: ADAM15 represents an tumor suppressor protein in melanoma. -- Abstract: In a mouse melanoma metastasis model it has been recently shown that ADAM15 overexpression in melanoma cells significantly reduced the number of metastatic nodules on the lung. Unfortunately, the expression of ADAM15 in human melanoma tissue has not been determined so far. In our study, we characterized the expression of ADAM15 in tissue micro-arrays of patients with primary melanoma with melanoma metastasis. ADAM15 was expressed in melanocytes and endothelial cells of benign nevi and melanoma tissue. Importantly, ADAM15 was significantly downregulated in melanoma metastasis compared to primary melanoma. We further demonstrate that IFN-{gamma} and TGF-{beta} downregulate ADAM15 protein levels in melanoma cells. To investigate the role of ADAM15 in melanoma progression, we overexpressed ADAM15 in melanoma cells. Importantly, overexpression of ADAM15 in melanoma cells reduced the migration, invasion and the anchorage dependent and independent cell growth of melanoma cells. In summary, the downregulation of ADAM15 plays an important role in melanoma progression and ADAM15 act as a tumorsuppressor in melanoma.},
doi = {10.1016/J.BBRC.2010.09.055},
url = {https://www.osti.gov/biblio/22202817}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 401,
place = {United States},
year = {Fri Oct 22 00:00:00 EDT 2010},
month = {Fri Oct 22 00:00:00 EDT 2010}
}