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Title: Bifurcate effects of glucose on caspase-independent cell death during hypoxia

Abstract

We investigated the effect of glucose on hypoxic death of rat cardiomyocyte-derived H9c2 cells and found that there is an optimal glucose concentration for protection against hypoxic cell death. Hypoxic cell death in the absence of glucose is accompanied by rapid ATP depletion, release of apoptosis-inducing factor from mitochondria, and nuclear chromatin condensation, all of which are inhibited by glucose in a dose-dependent manner. In contrast, excessive glucose also induces hypoxic cell death that is not accompanied by these events, suggesting a change in the mode of cell death between hypoxic cells with and without glucose supplementation.

Authors:
 [1]; ; ;  [1]
  1. Section of Forensic Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519 (Japan)
Publication Date:
OSTI Identifier:
22202631
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 396; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADP; ANOXIA; APOPTOSIS; ATP; CHROMATIN; CONCENTRATION RATIO; DEATH; FLUORESCENCE; GLUCOSE; LACTATE DEHYDROGENASE; MITOCHONDRIA; RATS; RIBOSE

Citation Formats

Aki, Toshihiko, E-mail: aki.legm@tmd.ac.jp, Nara, Akina, Funakoshi, Takeshi, and Uemura, Koichi. Bifurcate effects of glucose on caspase-independent cell death during hypoxia. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.04.122.
Aki, Toshihiko, E-mail: aki.legm@tmd.ac.jp, Nara, Akina, Funakoshi, Takeshi, & Uemura, Koichi. Bifurcate effects of glucose on caspase-independent cell death during hypoxia. United States. doi:10.1016/J.BBRC.2010.04.122.
Aki, Toshihiko, E-mail: aki.legm@tmd.ac.jp, Nara, Akina, Funakoshi, Takeshi, and Uemura, Koichi. Fri . "Bifurcate effects of glucose on caspase-independent cell death during hypoxia". United States. doi:10.1016/J.BBRC.2010.04.122.
@article{osti_22202631,
title = {Bifurcate effects of glucose on caspase-independent cell death during hypoxia},
author = {Aki, Toshihiko, E-mail: aki.legm@tmd.ac.jp and Nara, Akina and Funakoshi, Takeshi and Uemura, Koichi},
abstractNote = {We investigated the effect of glucose on hypoxic death of rat cardiomyocyte-derived H9c2 cells and found that there is an optimal glucose concentration for protection against hypoxic cell death. Hypoxic cell death in the absence of glucose is accompanied by rapid ATP depletion, release of apoptosis-inducing factor from mitochondria, and nuclear chromatin condensation, all of which are inhibited by glucose in a dose-dependent manner. In contrast, excessive glucose also induces hypoxic cell death that is not accompanied by these events, suggesting a change in the mode of cell death between hypoxic cells with and without glucose supplementation.},
doi = {10.1016/J.BBRC.2010.04.122},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 396,
place = {United States},
year = {Fri Jun 04 00:00:00 EDT 2010},
month = {Fri Jun 04 00:00:00 EDT 2010}
}
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