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Title: DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans

Abstract

Transcriptional regulators of the diphtheria toxin repressor (DtxR) family control the expression of genes involved in the uptake of iron and manganese, which is not only necessitous nutrients but also was suggested to be essential for intracellular redox cycling of microorganisms. We identified a unique DtxR homologue (DR2539) with special characteristics from Deinococcus radiodurans, which is known for its extreme resistance to radiation and oxidants. The dr2539 mutant showed higher resistance to hydrogen peroxide than the wild-type strain R1. Intracellular catalase activity assay and semiquantitative PCR analysis demonstrated that this DtxR is a negative regulator of catalase (katE). Furthermore, quantitative real-time PCR, global transcription profile and inductively coupled plasma-mass spectrometry analysis showed that the DtxR is involved in the regulation of antioxidant system by maintaining the intracellular Mn/Fe ion homeostasis of D. radiodurans. However, unlike the other DtxR homologues, the DtxR of D. radiodurans acts as a negative regulator of a Mn transporter gene (dr2283) and as a positive regulator of Fe-dependent transporter genes (dr1219, drb0125) in D. radiodurans.

Authors:
 [1];  [2];  [1]; ; ;  [3];  [1];  [1]
  1. Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029 (China)
  2. Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009 (China)
  3. Zhejiang Institute of Microbiology, Zhejiang Province, Hangzhou 310012 (China)
Publication Date:
OSTI Identifier:
22202618
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 396; Journal Issue: 2; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIOXIDANTS; ATP; CATALASE; DIPHTHERIA; HOMEOSTASIS; ICP MASS SPECTROSCOPY; IRON IONS; MICROORGANISMS; MUTANTS; NUTRIENTS; OXIDIZERS; POLYMERASE CHAIN REACTION; TOXINS; TRANSCRIPTION

Citation Formats

Chen, Huan, Zhejiang Institute of Microbiology, Zhejiang Province, Hangzhou 310012, Wu, Rongrong, Xu, Guangzhi, Fang, Xu, Qiu, Xiaoli, Guo, Hongyin, Tian, Bing, and Hua, Yuejin. DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.04.106.
Chen, Huan, Zhejiang Institute of Microbiology, Zhejiang Province, Hangzhou 310012, Wu, Rongrong, Xu, Guangzhi, Fang, Xu, Qiu, Xiaoli, Guo, Hongyin, Tian, Bing, & Hua, Yuejin. DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans. United States. doi:10.1016/J.BBRC.2010.04.106.
Chen, Huan, Zhejiang Institute of Microbiology, Zhejiang Province, Hangzhou 310012, Wu, Rongrong, Xu, Guangzhi, Fang, Xu, Qiu, Xiaoli, Guo, Hongyin, Tian, Bing, and Hua, Yuejin. Fri . "DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans". United States. doi:10.1016/J.BBRC.2010.04.106.
@article{osti_22202618,
title = {DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans},
author = {Chen, Huan and Zhejiang Institute of Microbiology, Zhejiang Province, Hangzhou 310012 and Wu, Rongrong and Xu, Guangzhi and Fang, Xu and Qiu, Xiaoli and Guo, Hongyin and Tian, Bing and Hua, Yuejin},
abstractNote = {Transcriptional regulators of the diphtheria toxin repressor (DtxR) family control the expression of genes involved in the uptake of iron and manganese, which is not only necessitous nutrients but also was suggested to be essential for intracellular redox cycling of microorganisms. We identified a unique DtxR homologue (DR2539) with special characteristics from Deinococcus radiodurans, which is known for its extreme resistance to radiation and oxidants. The dr2539 mutant showed higher resistance to hydrogen peroxide than the wild-type strain R1. Intracellular catalase activity assay and semiquantitative PCR analysis demonstrated that this DtxR is a negative regulator of catalase (katE). Furthermore, quantitative real-time PCR, global transcription profile and inductively coupled plasma-mass spectrometry analysis showed that the DtxR is involved in the regulation of antioxidant system by maintaining the intracellular Mn/Fe ion homeostasis of D. radiodurans. However, unlike the other DtxR homologues, the DtxR of D. radiodurans acts as a negative regulator of a Mn transporter gene (dr2283) and as a positive regulator of Fe-dependent transporter genes (dr1219, drb0125) in D. radiodurans.},
doi = {10.1016/J.BBRC.2010.04.106},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 396,
place = {United States},
year = {2010},
month = {5}
}