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Title: Melittin induces apoptotic features in Candida albicans

Abstract

Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.

Authors:
 [1];  [1]
  1. School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22202435
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 394; Journal Issue: 1; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CANDIDA; DNA; FLUORESCEIN; FLUORESCENCE; IODIDES; ISOTHIOCYANATES; LABELLING; MICROSCOPY; NUCLEAR FRAGMENTATION; OXYGEN; PEPTIDES

Citation Formats

Park, Cana, and Lee, Dong Gun, E-mail: dglee222@knu.ac.kr. Melittin induces apoptotic features in Candida albicans. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.02.138.
Park, Cana, & Lee, Dong Gun, E-mail: dglee222@knu.ac.kr. Melittin induces apoptotic features in Candida albicans. United States. doi:10.1016/J.BBRC.2010.02.138.
Park, Cana, and Lee, Dong Gun, E-mail: dglee222@knu.ac.kr. Fri . "Melittin induces apoptotic features in Candida albicans". United States. doi:10.1016/J.BBRC.2010.02.138.
@article{osti_22202435,
title = {Melittin induces apoptotic features in Candida albicans},
author = {Park, Cana and Lee, Dong Gun, E-mail: dglee222@knu.ac.kr},
abstractNote = {Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.},
doi = {10.1016/J.BBRC.2010.02.138},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 394,
place = {United States},
year = {2010},
month = {3}
}