Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro

Morizane, Ryuji; Monkawa, Toshiaki; Itoh, Hiroshi

doi:10.1016/J.BBRC.2009.10.148

Title: Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro

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Abstract

Embryonic stem (ES) cells which have the unlimited proliferative capacity and extensive differentiation potency can be an attractive source for kidney regeneration therapies. Recent breakthroughs in the generation of induced pluripotent stem (iPS) cells have provided with another potential source for the artificially-generated kidney. The purpose of this study is to know how to differentiate mouse ES and iPS cells into renal lineage. We used iPS cells from mouse fibroblasts by transfection of four transcription factors, namely Oct4, Sox2, c-Myc and Klf4. Real-time PCR showed that renal lineage markers were expressed in both ES and iPS cells after the induction of differentiation. It also showed that a tubular specific marker, KSP progressively increased to day 18, although the differentiation of iPS cells was slower than ES cells. The results indicated that renal lineage cells can be differentiated from both murine ES and iPS cells. Several inducing factors were tested whether they influenced on cell differentiation. In ES cells, both of GDNF and BMP7 enhanced the differentiation to metanephric mesenchyme, and Activin enhanced the differentiation of ES cells to tubular cells. Activin also enhanced the differentiation of iPS cells to tubular cells, although the enhancement was lower than in ES cells.more »« less

Authors:

Morizane, Ryuji ^[1]; Monkawa, Toshiaki ^[1]; Itoh, Hiroshi ^[1]

Department of Internal Medicine, Keio University School of Medicine, Tokyo (Japan)

Publication Date:: Fri Dec 25 00:00:00 EST 2009

OSTI Identifier:: 22199944

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 390; Journal Issue: 4; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CELL DIFFERENTIATION; FIBROBLASTS; IN VITRO; KIDNEYS; MICE; POLYMERASE CHAIN REACTION; REGENERATION; STEM CELLS; THERAPY; TRANSCRIPTION FACTORS

Citation Formats


                    Morizane, Ryuji, Monkawa, Toshiaki, and Itoh, Hiroshi. Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro.  United States: N. p., 2009. 
        Web.  doi:10.1016/J.BBRC.2009.10.148.

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                    Morizane, Ryuji, Monkawa, Toshiaki, & Itoh, Hiroshi. Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro.  United States.  https://doi.org/10.1016/J.BBRC.2009.10.148

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                    Morizane, Ryuji, Monkawa, Toshiaki, and Itoh, Hiroshi. 2009.  
        "Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro".  United States.  https://doi.org/10.1016/J.BBRC.2009.10.148.

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@article{osti_22199944,

  title        = {Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro},

  author       = {Morizane, Ryuji and Monkawa, Toshiaki and Itoh, Hiroshi},

  abstractNote = {Embryonic stem (ES) cells which have the unlimited proliferative capacity and extensive differentiation potency can be an attractive source for kidney regeneration therapies. Recent breakthroughs in the generation of induced pluripotent stem (iPS) cells have provided with another potential source for the artificially-generated kidney. The purpose of this study is to know how to differentiate mouse ES and iPS cells into renal lineage. We used iPS cells from mouse fibroblasts by transfection of four transcription factors, namely Oct4, Sox2, c-Myc and Klf4. Real-time PCR showed that renal lineage markers were expressed in both ES and iPS cells after the induction of differentiation. It also showed that a tubular specific marker, KSP progressively increased to day 18, although the differentiation of iPS cells was slower than ES cells. The results indicated that renal lineage cells can be differentiated from both murine ES and iPS cells. Several inducing factors were tested whether they influenced on cell differentiation. In ES cells, both of GDNF and BMP7 enhanced the differentiation to metanephric mesenchyme, and Activin enhanced the differentiation of ES cells to tubular cells. Activin also enhanced the differentiation of iPS cells to tubular cells, although the enhancement was lower than in ES cells. ES and iPS cells have a potential to differentiate to renal lineage cells, and they will be an attractive resource of kidney regeneration therapy. This differentiation is enhanced by Activin in both ES and iPS cells.},

  doi          = {10.1016/J.BBRC.2009.10.148},

  url          = {https://www.osti.gov/biblio/22199944},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 4,

  volume       = 390,

  place        = {United States},

  year         = {Fri Dec 25 00:00:00 EST 2009},

  month        = {Fri Dec 25 00:00:00 EST 2009}

}

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