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Title: SOX1 links the function of neural patterning and Notch signalling in the ventral spinal cord during the neuron-glial fate switch

Abstract

During neural development the transition from neurogenesis to gliogenesis, known as the neuron-glial ({Nu}/G) fate switch, requires the coordinated function of patterning factors, pro-glial factors and Notch signalling. How this process is coordinated in the embryonic spinal cord is poorly understood. Here, we demonstrate that during the N/G fate switch in the ventral spinal cord (vSC) SOX1 links the function of neural patterning and Notch signalling. We show that, SOX1 expression in the vSC is regulated by PAX6, NKX2.2 and Notch signalling in a domain-specific manner. We further show that SOX1 regulates the expression of Hes1 and that loss of Sox1 leads to enhanced production of oligodendrocyte precursors from the pMN. Finally, we show that Notch signalling functions upstream of SOX1 during this fate switch and is independently required for the acquisition of the glial fate perse by regulating Nuclear Factor I A expression in a PAX6/SOX1/HES1/HES5-independent manner. These data integrate functional roles of neural patterning factors, Notch signalling and SOX1 during gliogenesis.

Authors:
;  [1];  [2]; ; ; ; ; ;  [1]; ;  [3]; ;  [4];  [1];  [2]
  1. The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios, 2370 Nicosia (Cyprus)
  2. (Cyprus)
  3. James J. Peters VA Medical Center, Research and Development (3F22), 130 West Kingsbridge Road, Bronx, NY 10468 (United States)
  4. Wolfson Institute for Biomedical Research and Research Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT (United Kingdom)
Publication Date:
OSTI Identifier:
22199929
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 390; Journal Issue: 4; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL GROWTH; CELL DIFFERENTIATION; GROWTH FACTORS; NERVE CELLS; NERVE TISSUE; SPINAL CORD

Citation Formats

Genethliou, Nicholas, Panayiotou, Elena, Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia, Panayi, Helen, Orford, Michael, Mean, Richard, Lapathitis, George, Gill, Herman, Raoof, Sahir, Gasperi, Rita De, Elder, Gregory, Kessaris, Nicoletta, Richardson, William D., Malas, Stavros, E-mail: smalas@cing.ac.cy, and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia. SOX1 links the function of neural patterning and Notch signalling in the ventral spinal cord during the neuron-glial fate switch. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.08.154.
Genethliou, Nicholas, Panayiotou, Elena, Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia, Panayi, Helen, Orford, Michael, Mean, Richard, Lapathitis, George, Gill, Herman, Raoof, Sahir, Gasperi, Rita De, Elder, Gregory, Kessaris, Nicoletta, Richardson, William D., Malas, Stavros, E-mail: smalas@cing.ac.cy, & Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia. SOX1 links the function of neural patterning and Notch signalling in the ventral spinal cord during the neuron-glial fate switch. United States. doi:10.1016/J.BBRC.2009.08.154.
Genethliou, Nicholas, Panayiotou, Elena, Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia, Panayi, Helen, Orford, Michael, Mean, Richard, Lapathitis, George, Gill, Herman, Raoof, Sahir, Gasperi, Rita De, Elder, Gregory, Kessaris, Nicoletta, Richardson, William D., Malas, Stavros, E-mail: smalas@cing.ac.cy, and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia. Fri . "SOX1 links the function of neural patterning and Notch signalling in the ventral spinal cord during the neuron-glial fate switch". United States. doi:10.1016/J.BBRC.2009.08.154.
@article{osti_22199929,
title = {SOX1 links the function of neural patterning and Notch signalling in the ventral spinal cord during the neuron-glial fate switch},
author = {Genethliou, Nicholas and Panayiotou, Elena and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia and Panayi, Helen and Orford, Michael and Mean, Richard and Lapathitis, George and Gill, Herman and Raoof, Sahir and Gasperi, Rita De and Elder, Gregory and Kessaris, Nicoletta and Richardson, William D. and Malas, Stavros, E-mail: smalas@cing.ac.cy and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia},
abstractNote = {During neural development the transition from neurogenesis to gliogenesis, known as the neuron-glial ({Nu}/G) fate switch, requires the coordinated function of patterning factors, pro-glial factors and Notch signalling. How this process is coordinated in the embryonic spinal cord is poorly understood. Here, we demonstrate that during the N/G fate switch in the ventral spinal cord (vSC) SOX1 links the function of neural patterning and Notch signalling. We show that, SOX1 expression in the vSC is regulated by PAX6, NKX2.2 and Notch signalling in a domain-specific manner. We further show that SOX1 regulates the expression of Hes1 and that loss of Sox1 leads to enhanced production of oligodendrocyte precursors from the pMN. Finally, we show that Notch signalling functions upstream of SOX1 during this fate switch and is independently required for the acquisition of the glial fate perse by regulating Nuclear Factor I A expression in a PAX6/SOX1/HES1/HES5-independent manner. These data integrate functional roles of neural patterning factors, Notch signalling and SOX1 during gliogenesis.},
doi = {10.1016/J.BBRC.2009.08.154},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 390,
place = {United States},
year = {Fri Dec 25 00:00:00 EST 2009},
month = {Fri Dec 25 00:00:00 EST 2009}
}