Detection of single quantum dots in model organisms with sheet illumination microscopy

Journal Article · Fri Dec 18 00:00:00 EST 2009 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.10.036· OSTI ID:22199918

Friedrich, Mike; Nozadze, Revaz; Gan, Qiang; Zelman-Femiak, Monika; Ermolayev, Vladimir ^[1]; Wagner, Toni U. ^[2]

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1. Molecular Microscopy Group, Rudolf Virchow Center, University of Wuerzburg, Versbacher Str. 9, D-97078 Wuerzburg (Germany)
2. Institute of Physiological Chemistry I, Biocenter, University of Wuerzburg, Am Hubland, D-97074 Wuerzburg (Germany)

Single-molecule detection and tracking is important for observing biomolecule interactions in the microenvironment. Here we report selective plane illumination microscopy (SPIM) with single-molecule detection in living organisms, which enables fast imaging and single-molecule tracking and optical penetration beyond 300 {mu}m. We detected single nanocrystals in Drosophila larvae and zebrafish embryo. We also report our first tracking of single quantum dots during zebrafish development, which displays a transition from flow to confined motion prior to the blastula stage. The new SPIM setup represents a new technique, which enables fast single-molecule imaging and tracking in living systems.

• OSTI ID: 22199918
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 390, Issue 3; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DETECTION
DROSOPHILA
EMBRYOS
ILLUMINANCE
LARVAE
MICROSCOPY
MOLECULES
QUANTUM DOTS