Quantification of Randomly-methylated-{beta}-cyclodextrin effect on liposome: An ESR study
- Laboratory of Biomedical Spectroscopy, Department of Physics, Institute of Physics, B5, University of Liege, Sart-Tilman, B-4000 Liege (Belgium)
- Laboratory of Pharmaceutical Technology, Department of Pharmacy, CHU, B36, University of Liege, 1 Av. de l'Hopital, B-4000 Liege (Belgium)
In the present work, the effect of Randomly-methylated-{beta}-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-{alpha} phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9 {sup o}C).
- OSTI ID:
- 22199890
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 390, Issue 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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