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Title: Specific interaction of capsid protein and importin-{alpha}/{beta} influences West Nile virus production

Abstract

West Nile virus (WNV) capsid (C) protein has been shown to enter the nucleus of infected cells. However, the mechanism by which C protein enters the nucleus is unknown. In this study, we have unveiled for the first time that nuclear transport of WNV and Dengue virus C protein is mediated by their direct association with importin-{alpha}. This interplay is mediated by the consensus sequences of bipartite nuclear localization signal located between amino acid residues 85-101 together with amino acid residues 42 and 43 of C protein. Elucidation of biological significance of importin-{alpha}/C protein interaction demonstrated that the binding efficiency of this association influenced the nuclear entry of C protein and virus production. Collectively, this study illustrated the molecular mechanism by which the C protein of arthropod-borne flavivirus enters the nucleus and showed the importance of importin-{alpha}/C protein interaction in the context of flavivirus life-cycle.

Authors:
;  [1];  [1]
  1. Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597 (Singapore)
Publication Date:
OSTI Identifier:
22199860
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 389; Journal Issue: 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACIDS; ARTHROPODS; LIFE CYCLE; PROTEINS; SIGNALS; VIRUSES

Citation Formats

Bhuvanakantham, Raghavan, Chong, Mun-Keat, and Ng, Mah-Lee. Specific interaction of capsid protein and importin-{alpha}/{beta} influences West Nile virus production. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.08.108.
Bhuvanakantham, Raghavan, Chong, Mun-Keat, & Ng, Mah-Lee. Specific interaction of capsid protein and importin-{alpha}/{beta} influences West Nile virus production. United States. doi:10.1016/J.BBRC.2009.08.108.
Bhuvanakantham, Raghavan, Chong, Mun-Keat, and Ng, Mah-Lee. Fri . "Specific interaction of capsid protein and importin-{alpha}/{beta} influences West Nile virus production". United States. doi:10.1016/J.BBRC.2009.08.108.
@article{osti_22199860,
title = {Specific interaction of capsid protein and importin-{alpha}/{beta} influences West Nile virus production},
author = {Bhuvanakantham, Raghavan and Chong, Mun-Keat and Ng, Mah-Lee},
abstractNote = {West Nile virus (WNV) capsid (C) protein has been shown to enter the nucleus of infected cells. However, the mechanism by which C protein enters the nucleus is unknown. In this study, we have unveiled for the first time that nuclear transport of WNV and Dengue virus C protein is mediated by their direct association with importin-{alpha}. This interplay is mediated by the consensus sequences of bipartite nuclear localization signal located between amino acid residues 85-101 together with amino acid residues 42 and 43 of C protein. Elucidation of biological significance of importin-{alpha}/C protein interaction demonstrated that the binding efficiency of this association influenced the nuclear entry of C protein and virus production. Collectively, this study illustrated the molecular mechanism by which the C protein of arthropod-borne flavivirus enters the nucleus and showed the importance of importin-{alpha}/C protein interaction in the context of flavivirus life-cycle.},
doi = {10.1016/J.BBRC.2009.08.108},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 389,
place = {United States},
year = {2009},
month = {11}
}