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Title: Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast

Abstract

The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation in mitosis. Here we show that fission yeast casein kinase II (CK2) is required for this checkpoint function. In the CK2 mutants mitosis occurs in the presence of a spindle defect, and the spindle checkpoint protein Mad2p fails to localize to unattached kinetochores. The CK2 mutants are sensitive to the microtubule depolymerising drug thiabendazole, which is counteracted by ectopic expression of mad2{sup +}. The level of Mad2p is low in the CK2 mutants. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p.

Authors:
 [1];  [2]; ; ;  [1];  [3];  [1]
  1. Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan)
  2. Department of Chemistry, Shizuoka University, 836 Ohya, Suruga-ku, Sizuoka 422-8529 (Japan)
  3. Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601 (Japan)
Publication Date:
OSTI Identifier:
22199847
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 388; Journal Issue: 3; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CASEIN; CELL CYCLE; CHROMOSOMES; DRUGS; MEDICAL SURVEILLANCE; MICROTUBULES; MITOSIS; MUTANTS; YEASTS

Citation Formats

Shimada, Midori, Yamamoto, Ayumu, Murakami-Tonami, Yuko, Nakanishi, Makoto, Yoshida, Takashi, Aiba, Hirofumi, and Murakami, Hiroshi, E-mail: hmura@med.nagoya-cu.ac.jp. Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.08.030.
Shimada, Midori, Yamamoto, Ayumu, Murakami-Tonami, Yuko, Nakanishi, Makoto, Yoshida, Takashi, Aiba, Hirofumi, & Murakami, Hiroshi, E-mail: hmura@med.nagoya-cu.ac.jp. Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast. United States. doi:10.1016/J.BBRC.2009.08.030.
Shimada, Midori, Yamamoto, Ayumu, Murakami-Tonami, Yuko, Nakanishi, Makoto, Yoshida, Takashi, Aiba, Hirofumi, and Murakami, Hiroshi, E-mail: hmura@med.nagoya-cu.ac.jp. Fri . "Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast". United States. doi:10.1016/J.BBRC.2009.08.030.
@article{osti_22199847,
title = {Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast},
author = {Shimada, Midori and Yamamoto, Ayumu and Murakami-Tonami, Yuko and Nakanishi, Makoto and Yoshida, Takashi and Aiba, Hirofumi and Murakami, Hiroshi, E-mail: hmura@med.nagoya-cu.ac.jp},
abstractNote = {The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation in mitosis. Here we show that fission yeast casein kinase II (CK2) is required for this checkpoint function. In the CK2 mutants mitosis occurs in the presence of a spindle defect, and the spindle checkpoint protein Mad2p fails to localize to unattached kinetochores. The CK2 mutants are sensitive to the microtubule depolymerising drug thiabendazole, which is counteracted by ectopic expression of mad2{sup +}. The level of Mad2p is low in the CK2 mutants. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p.},
doi = {10.1016/J.BBRC.2009.08.030},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 388,
place = {United States},
year = {Fri Oct 23 00:00:00 EDT 2009},
month = {Fri Oct 23 00:00:00 EDT 2009}
}