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Title: Generation of an ABCG2{sup GFPn-puro} transgenic line - A tool to study ABCG2 expression in mice

Abstract

The ATP-binding cassette (ABC) transporter 2 (ABCG2) is expressed by stem cells in many organs and in stem cells of solid tumors. These cells are isolated based on the side population (SP) phenotype, a Hoechst 3342 dye efflux property believed to be conferred by ABCG2. Because of the limitations of this approach we generated transgenic mice that express Nuclear GFP (GFPn) coupled to the Puromycin-resistance gene, under the control of ABCG2 promoter/enhancer sequences. We show that ABCG2 is expressed in neural progenitors of the developing forebrain and spinal cord and in embryonic and adult endothelial cells of the brain. Using the neurosphere assay, we isolated tripotent ABCG2-expressing neural stem cells from embryonic mouse brain. This transgenic line is a powerful tool for studying the expression of ABCG2 in many tissues and for performing functional studies in different experimental settings.

Authors:
; ; ; ; ;  [1];  [1]
  1. The Cyprus Institute of Neurology and Genetics, Airport Avenue, No. 6, Agios Dometios 2370, Nicosia (Cyprus)
Publication Date:
OSTI Identifier:
22199726
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 384; Journal Issue: 2; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; ATP; BRAIN; NEOPLASMS; PHENOTYPE; PROMOTERS; PUROMYCIN; SPINAL CORD; STEM CELLS; TRANSGENIC MICE; UVEA

Citation Formats

Orford, Michael, Mean, Richard, Lapathitis, George, Genethliou, Nicholas, Panayiotou, Elena, Panayi, Helen, Malas, Stavros, and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia. Generation of an ABCG2{sup GFPn-puro} transgenic line - A tool to study ABCG2 expression in mice. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.04.089.
Orford, Michael, Mean, Richard, Lapathitis, George, Genethliou, Nicholas, Panayiotou, Elena, Panayi, Helen, Malas, Stavros, & Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia. Generation of an ABCG2{sup GFPn-puro} transgenic line - A tool to study ABCG2 expression in mice. United States. https://doi.org/10.1016/J.BBRC.2009.04.089
Orford, Michael, Mean, Richard, Lapathitis, George, Genethliou, Nicholas, Panayiotou, Elena, Panayi, Helen, Malas, Stavros, and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia. 2009. "Generation of an ABCG2{sup GFPn-puro} transgenic line - A tool to study ABCG2 expression in mice". United States. https://doi.org/10.1016/J.BBRC.2009.04.089.
@article{osti_22199726,
title = {Generation of an ABCG2{sup GFPn-puro} transgenic line - A tool to study ABCG2 expression in mice},
author = {Orford, Michael and Mean, Richard and Lapathitis, George and Genethliou, Nicholas and Panayiotou, Elena and Panayi, Helen and Malas, Stavros and Department of Biological Sciences, University of Cyprus, P.O. Box 20537, 1678 Nicosia},
abstractNote = {The ATP-binding cassette (ABC) transporter 2 (ABCG2) is expressed by stem cells in many organs and in stem cells of solid tumors. These cells are isolated based on the side population (SP) phenotype, a Hoechst 3342 dye efflux property believed to be conferred by ABCG2. Because of the limitations of this approach we generated transgenic mice that express Nuclear GFP (GFPn) coupled to the Puromycin-resistance gene, under the control of ABCG2 promoter/enhancer sequences. We show that ABCG2 is expressed in neural progenitors of the developing forebrain and spinal cord and in embryonic and adult endothelial cells of the brain. Using the neurosphere assay, we isolated tripotent ABCG2-expressing neural stem cells from embryonic mouse brain. This transgenic line is a powerful tool for studying the expression of ABCG2 in many tissues and for performing functional studies in different experimental settings.},
doi = {10.1016/J.BBRC.2009.04.089},
url = {https://www.osti.gov/biblio/22199726}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 384,
place = {United States},
year = {Fri Jun 26 00:00:00 EDT 2009},
month = {Fri Jun 26 00:00:00 EDT 2009}
}