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Title: Identification of two functional nuclear localization signals in the capsid protein of duck circovirus

Abstract

The capsid protein (CP) of duck circovirus (DuCV) is the major immunogenic protein and has a high proportion of arginine residues concentrated at the N terminus of the protein, which inhibits efficient mRNA translation in prokaryotic expression systems. In this study, we investigated the subcellular distribution of DuCV CP expressed via recombinant baculoviruses in Sf9 cells and the DNA binding activities of the truncated recombinant DuCV CPs. The results showed that two independent bipartite nuclear localization signals (NLSs) situated at N-terminal 1-17 and 18-36 amino acid residue of the CP. Moreover, two expression level regulatory signals (ELRSs) and two DNA binding signals (DBSs) were also mapped to the N terminus of the protein and overlapped with the two NLSs. The ability of CP to bind DNA, coupled with the karyophilic nature of this protein, strongly suggests that it may be responsible for nuclear targeting of the viral genome.

Authors:
; ;  [1];  [2]; ;  [1];  [3];  [1];  [1]
  1. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, Taian 271018 (China)
  2. College of Veterinary Medicine, Northwest A and F University, Shanxi, Yangling 712100 (China)
  3. Department of Basic Medical Sciences, Taishan Medical College, Shandong, Taian 271000 (China)
Publication Date:
OSTI Identifier:
22150097
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 436; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARGININE; DNA; DUCKS; MESSENGER-RNA; PROTEINS; SUBCELLULAR DISTRIBUTION

Citation Formats

Xiang, Qi-Wang, Zou, Jin-Feng, Wang, Xin, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Sun, Ya-Ni, Gao, Ji-Ming, Xie, Zhi-Jing, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Wang, Yu, Zhu, Yan-Li, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Jiang, Shi-Jin, and Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018. Identification of two functional nuclear localization signals in the capsid protein of duck circovirus. United States: N. p., 2013. Web. doi:10.1016/J.VIROL.2012.10.035.
Xiang, Qi-Wang, Zou, Jin-Feng, Wang, Xin, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Sun, Ya-Ni, Gao, Ji-Ming, Xie, Zhi-Jing, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Wang, Yu, Zhu, Yan-Li, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Jiang, Shi-Jin, & Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018. Identification of two functional nuclear localization signals in the capsid protein of duck circovirus. United States. doi:10.1016/J.VIROL.2012.10.035.
Xiang, Qi-Wang, Zou, Jin-Feng, Wang, Xin, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Sun, Ya-Ni, Gao, Ji-Ming, Xie, Zhi-Jing, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Wang, Yu, Zhu, Yan-Li, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018, Jiang, Shi-Jin, and Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018. Tue . "Identification of two functional nuclear localization signals in the capsid protein of duck circovirus". United States. doi:10.1016/J.VIROL.2012.10.035.
@article{osti_22150097,
title = {Identification of two functional nuclear localization signals in the capsid protein of duck circovirus},
author = {Xiang, Qi-Wang and Zou, Jin-Feng and Wang, Xin and Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018 and Sun, Ya-Ni and Gao, Ji-Ming and Xie, Zhi-Jing and Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018 and Wang, Yu and Zhu, Yan-Li and Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018 and Jiang, Shi-Jin and Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong, Taian 271018},
abstractNote = {The capsid protein (CP) of duck circovirus (DuCV) is the major immunogenic protein and has a high proportion of arginine residues concentrated at the N terminus of the protein, which inhibits efficient mRNA translation in prokaryotic expression systems. In this study, we investigated the subcellular distribution of DuCV CP expressed via recombinant baculoviruses in Sf9 cells and the DNA binding activities of the truncated recombinant DuCV CPs. The results showed that two independent bipartite nuclear localization signals (NLSs) situated at N-terminal 1-17 and 18-36 amino acid residue of the CP. Moreover, two expression level regulatory signals (ELRSs) and two DNA binding signals (DBSs) were also mapped to the N terminus of the protein and overlapped with the two NLSs. The ability of CP to bind DNA, coupled with the karyophilic nature of this protein, strongly suggests that it may be responsible for nuclear targeting of the viral genome.},
doi = {10.1016/J.VIROL.2012.10.035},
journal = {Virology},
issn = {0042-6822},
number = 1,
volume = 436,
place = {United States},
year = {2013},
month = {2}
}