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Title: Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus

Abstract

Grapevine rupestris stem pitting-associated virus (GRSPaV, genus Foveavirus, family Betaflexiviridae) is one of the most prevalent viruses in grapevines and is associated with three distinct diseases: rupestris stem pitting, vein necrosis and Syrah decline. Little is known about the biology and pathological properties of GRSPaV. In this work, we engineered a full-length infectious cDNA clone for GRSPaV and a GFP-tagged variant, both under the transcriptional control of Cauliflower mosaic virus 35 S promoter. We demonstrated that these cDNA clones were infectious in grapevines and Nicotiana benthamiana through fluorescence microscopy, RT-PCR, Western blotting and immuno electron microscopy. Interestingly, GRSPaV does not cause systemic infection in four of the most commonly used herbaceous plants, even in the presence of the movement proteins of two other viruses which are known to complement numerous movement-defective viruses. These infectious clones are the first of members of Foveavirus which would allow further investigations into mechanisms governing different aspects of replication for GRSPaV and perhaps related viruses.

Authors:
 [1]; ; ;  [1]; ;  [2]
  1. Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario, Canada N1G2W1 (Canada)
  2. The Plant Pathology Department-The Virology Unit, Plant Protection Institute, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250 (Israel)
Publication Date:
OSTI Identifier:
22150095
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 435; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BRASSICA; ELECTRON MICROSCOPY; FLUORESCENCE; NICOTIANA; POLYMERASE CHAIN REACTION; VIRUSES

Citation Formats

Meng, Baozhong, Venkataraman, Srividhya, Li, Caihong, Wang, Weizhou, Dayan-Glick, Cathy, and Mawassi, Munir. Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus. United States: N. p., 2013. Web. doi:10.1016/J.VIROL.2012.09.045.
Meng, Baozhong, Venkataraman, Srividhya, Li, Caihong, Wang, Weizhou, Dayan-Glick, Cathy, & Mawassi, Munir. Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus. United States. doi:10.1016/J.VIROL.2012.09.045.
Meng, Baozhong, Venkataraman, Srividhya, Li, Caihong, Wang, Weizhou, Dayan-Glick, Cathy, and Mawassi, Munir. Sun . "Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus". United States. doi:10.1016/J.VIROL.2012.09.045.
@article{osti_22150095,
title = {Construction and biological activities of the first infectious cDNA clones of the genus Foveavirus},
author = {Meng, Baozhong and Venkataraman, Srividhya and Li, Caihong and Wang, Weizhou and Dayan-Glick, Cathy and Mawassi, Munir},
abstractNote = {Grapevine rupestris stem pitting-associated virus (GRSPaV, genus Foveavirus, family Betaflexiviridae) is one of the most prevalent viruses in grapevines and is associated with three distinct diseases: rupestris stem pitting, vein necrosis and Syrah decline. Little is known about the biology and pathological properties of GRSPaV. In this work, we engineered a full-length infectious cDNA clone for GRSPaV and a GFP-tagged variant, both under the transcriptional control of Cauliflower mosaic virus 35 S promoter. We demonstrated that these cDNA clones were infectious in grapevines and Nicotiana benthamiana through fluorescence microscopy, RT-PCR, Western blotting and immuno electron microscopy. Interestingly, GRSPaV does not cause systemic infection in four of the most commonly used herbaceous plants, even in the presence of the movement proteins of two other viruses which are known to complement numerous movement-defective viruses. These infectious clones are the first of members of Foveavirus which would allow further investigations into mechanisms governing different aspects of replication for GRSPaV and perhaps related viruses.},
doi = {10.1016/J.VIROL.2012.09.045},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 435,
place = {United States},
year = {2013},
month = {1}
}