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Title: Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin

Abstract

A comparative analysis of the intracellular distribution of avian reovirus (ARV) core protein sigmaA in cells of avian and mammalian origin revealed that, whereas the viral protein accumulates in the cytoplasm and nucleolus of avian cells, most sigmaA concentrates in the nucleoplasm of mammalian cells in tight association with the insoluble nuclear matrix fraction. Our results further showed that sigmaA becomes arrested in the nucleoplasm of mammalian cells via association with mammalian cell-specific factors and that this association prevents nucleolar targeting. Inhibition of RNA polymerase II activity, but not of RNA polymerase I activity, in infected mammalian cells induces nucleus-to-cytoplasm sigmaA translocation through a CRM1- and RanGTP-dependent mechanism, yet a heterokaryon assay suggests that sigmaA does not shuttle between the nucleus and cytoplasm. The scarcity of sigmaA in cytoplasmic viral factories of infected mammalian cells could be one of the factors contributing to limited ARV replication in mammalian cells.

Authors:
; ;  [1];  [1]
  1. Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, y Centro Singular de Investigacion en Quimica Biologica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, 15782-Santiago de Compostela (Spain)
Publication Date:
OSTI Identifier:
22150075
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 432; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CYTOPLASM; INHIBITION; NUCLEAR MATRIX; PROTEINS; RNA; TRANSLOCATION

Citation Formats

Vazquez-Iglesias, Lorena, Lostale-Seijo, Irene, Martinez-Costas, Jose, and Benavente, Javier, E-mail: franciscojavier.benavente@usc.es. Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.07.007.
Vazquez-Iglesias, Lorena, Lostale-Seijo, Irene, Martinez-Costas, Jose, & Benavente, Javier, E-mail: franciscojavier.benavente@usc.es. Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin. United States. doi:10.1016/J.VIROL.2012.07.007.
Vazquez-Iglesias, Lorena, Lostale-Seijo, Irene, Martinez-Costas, Jose, and Benavente, Javier, E-mail: franciscojavier.benavente@usc.es. Thu . "Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin". United States. doi:10.1016/J.VIROL.2012.07.007.
@article{osti_22150075,
title = {Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin},
author = {Vazquez-Iglesias, Lorena and Lostale-Seijo, Irene and Martinez-Costas, Jose and Benavente, Javier, E-mail: franciscojavier.benavente@usc.es},
abstractNote = {A comparative analysis of the intracellular distribution of avian reovirus (ARV) core protein sigmaA in cells of avian and mammalian origin revealed that, whereas the viral protein accumulates in the cytoplasm and nucleolus of avian cells, most sigmaA concentrates in the nucleoplasm of mammalian cells in tight association with the insoluble nuclear matrix fraction. Our results further showed that sigmaA becomes arrested in the nucleoplasm of mammalian cells via association with mammalian cell-specific factors and that this association prevents nucleolar targeting. Inhibition of RNA polymerase II activity, but not of RNA polymerase I activity, in infected mammalian cells induces nucleus-to-cytoplasm sigmaA translocation through a CRM1- and RanGTP-dependent mechanism, yet a heterokaryon assay suggests that sigmaA does not shuttle between the nucleus and cytoplasm. The scarcity of sigmaA in cytoplasmic viral factories of infected mammalian cells could be one of the factors contributing to limited ARV replication in mammalian cells.},
doi = {10.1016/J.VIROL.2012.07.007},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 432,
place = {United States},
year = {2012},
month = {10}
}