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Title: The herpes simplex virus 1 U{sub S}3 regulates phospholipid synthesis

Abstract

Herpes simplex virus type 1 capsids bud at nuclear and Golgi membranes for envelopment by phospholipid bilayers. In the absence of U{sub S}3, nuclear membranes form multiple folds harboring virions that suggests disturbance in membrane turnover. Therefore, we investigated phospholipid metabolism in cells infected with the U{sub S}3 deletion mutant R7041({Delta}U{sub S}3), and quantified membranes involved in viral envelopment. We report that (i) [{sup 3}H]-choline incorporation into nuclear membranes and cytoplasmic membranes was enhanced peaking at 12 or 20 h post inoculation with wild type HSV-1 and R7041({Delta}U{sub S}3), respectively, (ii) the surface area of nuclear membranes increased until 24 h of R7041({Delta}U{sub S}3) infection forming folds that equaled {approx}45% of the nuclear surface, (iii) the surface area of viral envelopes between nuclear membranes equaled {approx}2400 R7041({Delta}U{sub S}3) virions per cell, and (iv) during R7041({Delta}U{sub S}3) infection, the Golgi complex expanded dramatically. The data indicate that U{sub S}3 plays a significant role in regulation of membrane biosynthesis.

Authors:
 [1];  [2];  [3];  [1]; ;  [2]
  1. Institute of Veterinary Anatomy, University of Zuerich (Switzerland)
  2. Institute of Virology, University of Zuerich (Switzerland)
  3. Institute for Parasitology, University of Zuerich (Switzerland)
Publication Date:
OSTI Identifier:
22150072
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 432; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOSYNTHESIS; CHOLINE; GOLGI COMPLEXES; HERPES SIMPLEX; MEMBRANES; PHOSPHOLIPIDS; SURFACE AREA; TRANSMISSION ELECTRON MICROSCOPY; VIRUSES

Citation Formats

Wild, Peter, Institute of Virology, University of Zuerich, Oliveira, Anna Paula de, Sonda, Sabrina, Schraner, Elisabeth M., Institute of Virology, University of Zuerich, Ackermann, Mathias, and Tobler, Kurt. The herpes simplex virus 1 U{sub S}3 regulates phospholipid synthesis. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.06.020.
Wild, Peter, Institute of Virology, University of Zuerich, Oliveira, Anna Paula de, Sonda, Sabrina, Schraner, Elisabeth M., Institute of Virology, University of Zuerich, Ackermann, Mathias, & Tobler, Kurt. The herpes simplex virus 1 U{sub S}3 regulates phospholipid synthesis. United States. https://doi.org/10.1016/J.VIROL.2012.06.020
Wild, Peter, Institute of Virology, University of Zuerich, Oliveira, Anna Paula de, Sonda, Sabrina, Schraner, Elisabeth M., Institute of Virology, University of Zuerich, Ackermann, Mathias, and Tobler, Kurt. 2012. "The herpes simplex virus 1 U{sub S}3 regulates phospholipid synthesis". United States. https://doi.org/10.1016/J.VIROL.2012.06.020.
@article{osti_22150072,
title = {The herpes simplex virus 1 U{sub S}3 regulates phospholipid synthesis},
author = {Wild, Peter and Institute of Virology, University of Zuerich and Oliveira, Anna Paula de and Sonda, Sabrina and Schraner, Elisabeth M. and Institute of Virology, University of Zuerich and Ackermann, Mathias and Tobler, Kurt},
abstractNote = {Herpes simplex virus type 1 capsids bud at nuclear and Golgi membranes for envelopment by phospholipid bilayers. In the absence of U{sub S}3, nuclear membranes form multiple folds harboring virions that suggests disturbance in membrane turnover. Therefore, we investigated phospholipid metabolism in cells infected with the U{sub S}3 deletion mutant R7041({Delta}U{sub S}3), and quantified membranes involved in viral envelopment. We report that (i) [{sup 3}H]-choline incorporation into nuclear membranes and cytoplasmic membranes was enhanced peaking at 12 or 20 h post inoculation with wild type HSV-1 and R7041({Delta}U{sub S}3), respectively, (ii) the surface area of nuclear membranes increased until 24 h of R7041({Delta}U{sub S}3) infection forming folds that equaled {approx}45% of the nuclear surface, (iii) the surface area of viral envelopes between nuclear membranes equaled {approx}2400 R7041({Delta}U{sub S}3) virions per cell, and (iv) during R7041({Delta}U{sub S}3) infection, the Golgi complex expanded dramatically. The data indicate that U{sub S}3 plays a significant role in regulation of membrane biosynthesis.},
doi = {10.1016/J.VIROL.2012.06.020},
url = {https://www.osti.gov/biblio/22150072}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 432,
place = {United States},
year = {Thu Oct 25 00:00:00 EDT 2012},
month = {Thu Oct 25 00:00:00 EDT 2012}
}