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Title: The early UL31 gene of equine herpesvirus 1 encodes a single-stranded DNA-binding protein that has a nuclear localization signal sequence at the C-terminus

Abstract

The amino acid sequence of the UL31 protein (UL31P) of equine herpesvirus 1 (EHV-1) has homology to that of the ICP8 of herpes simplex virus type 1 (HSV-1). Here we show that the UL31 gene is synergistically trans-activated by the IEP and the UL5P (EICP27). Detection of the UL31 RNA transcript and the UL31P in EHV-1-infected cells at 6 h post-infection (hpi) as well as metabolic inhibition assays indicated that UL31 is an early gene. The UL31P preferentially bound to single-stranded DNA over double-stranded DNA in gel shift assays. Subcellular localization of the green fluorescent protein (GFP)-UL31 fusion proteins revealed that the C-terminal 32 amino acid residues of the UL31P are responsible for the nuclear localization. These findings may contribute to defining the role of the UL31P single-stranded DNA-binding protein in EHV-1 DNA replication.

Authors:
; ;  [1];  [1]
  1. Department of Microbiology and Immunology, Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932 (United States)
Publication Date:
OSTI Identifier:
22150071
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 432; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; AMINO ACIDS; DNA; DNA REPLICATION; FLUORESCENCE; GELS; GENES; HERPES SIMPLEX; PROTEINS; RNA; VIRUSES

Citation Formats

Kim, Seongman, Chul Ahn, Byung, O'Callaghan, Dennis J., and Kim, Seong Kee, E-mail: skim1@lsuhsc.edu. The early UL31 gene of equine herpesvirus 1 encodes a single-stranded DNA-binding protein that has a nuclear localization signal sequence at the C-terminus. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.05.031.
Kim, Seongman, Chul Ahn, Byung, O'Callaghan, Dennis J., & Kim, Seong Kee, E-mail: skim1@lsuhsc.edu. The early UL31 gene of equine herpesvirus 1 encodes a single-stranded DNA-binding protein that has a nuclear localization signal sequence at the C-terminus. United States. doi:10.1016/J.VIROL.2012.05.031.
Kim, Seongman, Chul Ahn, Byung, O'Callaghan, Dennis J., and Kim, Seong Kee, E-mail: skim1@lsuhsc.edu. Thu . "The early UL31 gene of equine herpesvirus 1 encodes a single-stranded DNA-binding protein that has a nuclear localization signal sequence at the C-terminus". United States. doi:10.1016/J.VIROL.2012.05.031.
@article{osti_22150071,
title = {The early UL31 gene of equine herpesvirus 1 encodes a single-stranded DNA-binding protein that has a nuclear localization signal sequence at the C-terminus},
author = {Kim, Seongman and Chul Ahn, Byung and O'Callaghan, Dennis J. and Kim, Seong Kee, E-mail: skim1@lsuhsc.edu},
abstractNote = {The amino acid sequence of the UL31 protein (UL31P) of equine herpesvirus 1 (EHV-1) has homology to that of the ICP8 of herpes simplex virus type 1 (HSV-1). Here we show that the UL31 gene is synergistically trans-activated by the IEP and the UL5P (EICP27). Detection of the UL31 RNA transcript and the UL31P in EHV-1-infected cells at 6 h post-infection (hpi) as well as metabolic inhibition assays indicated that UL31 is an early gene. The UL31P preferentially bound to single-stranded DNA over double-stranded DNA in gel shift assays. Subcellular localization of the green fluorescent protein (GFP)-UL31 fusion proteins revealed that the C-terminal 32 amino acid residues of the UL31P are responsible for the nuclear localization. These findings may contribute to defining the role of the UL31P single-stranded DNA-binding protein in EHV-1 DNA replication.},
doi = {10.1016/J.VIROL.2012.05.031},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 432,
place = {United States},
year = {2012},
month = {10}
}