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Title: Adenoviral protein V promotes a process of viral assembly through nucleophosmin 1

Abstract

Adenoviral infection induces nucleoplasmic redistribution of a nucleolar nucleophosmin 1/NPM1/B23.1. NPM1 is preferentially localized in the nucleoli of normal cells, whereas it is also present at the nuclear matrix in cancer cells. However, the biological roles of NPM1 during infection are unknown. Here, by analyzing a pV-deletion mutant, Ad5-dV/TSB, we demonstrate that pV promotes the NPM1 translocation from the nucleoli to the nucleoplasm in normal cells, and the NPM1 translocation is correlated with adenoviral replication. Lack of pV causes a dramatic reduction of adenoviral replication in normal cells, but not cancer cells, and Ad5-dV/TSB was defective in viral assembly in normal cells. NPM1 knockdown inhibits adenoviral replication, suggesting an involvement of NPM1 in adenoviral biology. Further, we show that NPM1 interacts with empty adenovirus particles which are an intermediate during virion maturation by immunoelectron microscopy. Collectively, these data implicate that pV participates in a process of viral assembly through NPM1.

Authors:
; ;  [1];  [2];  [3];  [1];  [4]
  1. Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, and Surgery, University of Alabama at Birmingham, Birmingham, AL 35294 (United States)
  2. Massachusetts General Hospital, Pathology Service, 55 Fruit St.-GRJ 249, Boston, MA 02114 (United States)
  3. School of Cellular and Molecular Medicine, Medical Sciences Building, University of Bristol, Bristol BS8 1TD (United Kingdom)
  4. (United States)
Publication Date:
OSTI Identifier:
22150070
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 432; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADENOVIRUS; GENE THERAPY; MUTANTS; NEOPLASMS; NUCLEAR MATRIX; NUCLEOLI; PROTEINS; TRANSLOCATION

Citation Formats

Ugai, Hideyo, Dobbins, George C., Wang, Minghui, Le, Long P., Matthews, David A., Curiel, David T., E-mail: dcuriel@radonc.wustl.edu, and The Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294. Adenoviral protein V promotes a process of viral assembly through nucleophosmin 1. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.05.028.
Ugai, Hideyo, Dobbins, George C., Wang, Minghui, Le, Long P., Matthews, David A., Curiel, David T., E-mail: dcuriel@radonc.wustl.edu, & The Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294. Adenoviral protein V promotes a process of viral assembly through nucleophosmin 1. United States. doi:10.1016/J.VIROL.2012.05.028.
Ugai, Hideyo, Dobbins, George C., Wang, Minghui, Le, Long P., Matthews, David A., Curiel, David T., E-mail: dcuriel@radonc.wustl.edu, and The Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294. Thu . "Adenoviral protein V promotes a process of viral assembly through nucleophosmin 1". United States. doi:10.1016/J.VIROL.2012.05.028.
@article{osti_22150070,
title = {Adenoviral protein V promotes a process of viral assembly through nucleophosmin 1},
author = {Ugai, Hideyo and Dobbins, George C. and Wang, Minghui and Le, Long P. and Matthews, David A. and Curiel, David T., E-mail: dcuriel@radonc.wustl.edu and The Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294},
abstractNote = {Adenoviral infection induces nucleoplasmic redistribution of a nucleolar nucleophosmin 1/NPM1/B23.1. NPM1 is preferentially localized in the nucleoli of normal cells, whereas it is also present at the nuclear matrix in cancer cells. However, the biological roles of NPM1 during infection are unknown. Here, by analyzing a pV-deletion mutant, Ad5-dV/TSB, we demonstrate that pV promotes the NPM1 translocation from the nucleoli to the nucleoplasm in normal cells, and the NPM1 translocation is correlated with adenoviral replication. Lack of pV causes a dramatic reduction of adenoviral replication in normal cells, but not cancer cells, and Ad5-dV/TSB was defective in viral assembly in normal cells. NPM1 knockdown inhibits adenoviral replication, suggesting an involvement of NPM1 in adenoviral biology. Further, we show that NPM1 interacts with empty adenovirus particles which are an intermediate during virion maturation by immunoelectron microscopy. Collectively, these data implicate that pV participates in a process of viral assembly through NPM1.},
doi = {10.1016/J.VIROL.2012.05.028},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 432,
place = {United States},
year = {2012},
month = {10}
}