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Title: The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses

Abstract

Influenza A are nuclear replicating viruses which hijack host machineries in order to achieve optimal infection. Numerous functional virus-host interactions have now been characterized, but little information has been gathered concerning their link to the virally induced remodeling of the host cellular architecture. In this study, we infected cells with several human and avian influenza viruses and we have analyzed their ultrastructural modifications by using electron and confocal microscopy. We discovered that infections lead to a major and systematic disruption of nucleoli and the formation of a large number of diverse viral structures showing specificity that depended on the subtype origin and genomic composition of viruses. We identified NS1 and M1 proteins as the main actors in the remodeling of the host ultra-structure and our results suggest that each influenza A virus strain could be associated with a specific cellular fingerprint, possibly correlated to the functional properties of their viral components.

Authors:
; ; ;  [1];  [2]; ;  [1];  [3];  [4];  [5];
  1. Equipe VirCell, Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Universite de Lyon, Universite Claude Bernard Lyon 1, Hospices Civils de Lyon, Faculte de medecine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon (France)
  2. Laboratoire de Virologie, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, 59 boulevard Pinel, F-69677 Bron Cedex, Lyon (France)
  3. Division of Influenza/Respiratory Viruses, Robert Koch Institute, Nordufer 20, D-13353 Berlin (Germany)
  4. Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Universite de Lyon, Universite Claude Bernard Lyon 1, Hospices Civils de Lyon, Faculte de medecine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon (France)
  5. Institut Pasteur, Unite de Genetique Moleculaire des Virus Respiratoires, URA CNRS 3015, EA302 Universite Paris Diderot, Paris (France)
Publication Date:
OSTI Identifier:
22150069
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 432; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; INFLUENZA; INFLUENZA VIRUSES; MICROSCOPY; PROTEINS; SPECIFICITY

Citation Formats

Terrier, Olivier, Moules, Vincent, Carron, Coralie, Cartet, Gaeelle, Frobert, Emilie, Yver, Matthieu, Traversier, Aurelien, Wolff, Thorsten, Riteau, Beatrice, Naffakh, Nadia, and others, and. The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.05.019.
Terrier, Olivier, Moules, Vincent, Carron, Coralie, Cartet, Gaeelle, Frobert, Emilie, Yver, Matthieu, Traversier, Aurelien, Wolff, Thorsten, Riteau, Beatrice, Naffakh, Nadia, & others, and. The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses. United States. doi:10.1016/J.VIROL.2012.05.019.
Terrier, Olivier, Moules, Vincent, Carron, Coralie, Cartet, Gaeelle, Frobert, Emilie, Yver, Matthieu, Traversier, Aurelien, Wolff, Thorsten, Riteau, Beatrice, Naffakh, Nadia, and others, and. Wed . "The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses". United States. doi:10.1016/J.VIROL.2012.05.019.
@article{osti_22150069,
title = {The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses},
author = {Terrier, Olivier and Moules, Vincent and Carron, Coralie and Cartet, Gaeelle and Frobert, Emilie and Yver, Matthieu and Traversier, Aurelien and Wolff, Thorsten and Riteau, Beatrice and Naffakh, Nadia and others, and},
abstractNote = {Influenza A are nuclear replicating viruses which hijack host machineries in order to achieve optimal infection. Numerous functional virus-host interactions have now been characterized, but little information has been gathered concerning their link to the virally induced remodeling of the host cellular architecture. In this study, we infected cells with several human and avian influenza viruses and we have analyzed their ultrastructural modifications by using electron and confocal microscopy. We discovered that infections lead to a major and systematic disruption of nucleoli and the formation of a large number of diverse viral structures showing specificity that depended on the subtype origin and genomic composition of viruses. We identified NS1 and M1 proteins as the main actors in the remodeling of the host ultra-structure and our results suggest that each influenza A virus strain could be associated with a specific cellular fingerprint, possibly correlated to the functional properties of their viral components.},
doi = {10.1016/J.VIROL.2012.05.019},
journal = {Virology},
issn = {0042-6822},
number = 1,
volume = 432,
place = {United States},
year = {2012},
month = {10}
}