skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Expression of hPNAS-4 Radiosensitizes Lewis Lung Cancer

Abstract

Purpose: This study aimed to transfer the hPNAS-4 gene, a novel apoptosis-related human gene, into Lewis lung cancer (LL2) and observe its radiosensitive effect on radiation therapy in vitro and in vivo. Methods and Materials: The hPNAS-4 gene was transfected into LL2 cells, and its expression was detected via western blot. Colony formation assay and flow cytometry were used to detect the growth and apoptosis of cells treated with irradiation/PNAS-4 in vitro. The hPNAS-4 gene was transferred into LL2-bearing mice through tail vein injection of the liposome/gene complex. The tumor volumes were recorded after radiation therapy. Proliferating cell nuclear antigen (PCNA) immunohistochemistry staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were performed to detect the tumor cell growth and apoptosis in vivo. Results: The hPNAS-4 gene was successfully transferred into LL2 cells and tumor tissue, and its overexpressions were confirmed via western blot analysis. Compared with the control, empty plasmid, hPNAS-4, radiation, and empty plasmid plus radiation groups, the hPNAS-4 plus radiation group more significantly inhibited growth and enhanced apoptosis of LL2 cells in vitro and in vivo (P<.05). Conclusions: The hPNAS-4 gene was successfully transferred into LL2 cells and tumor tissue and was expressed in bothmore » LL2 cell and tumor tissue. The hPNAS-4 gene therapy significantly enhanced growth inhibition and apoptosis of LL2 tumor cells by radiation therapy in vitro and in vivo. Therefore, it may be a potential radiosensitive treatment of radiation therapy for lung cancer.« less

Authors:
 [1];  [2];  [1]; ;  [2]; ; ; ; ;  [1];  [2];  [1]
  1. Department of Radiation Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province (China)
  2. State Key Laboratory of Biotherapy, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province (China)
Publication Date:
OSTI Identifier:
22149652
Resource Type:
Journal Article
Journal Name:
International Journal of Radiation Oncology, Biology and Physics
Additional Journal Information:
Journal Volume: 84; Journal Issue: 4; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0360-3016
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIGENS; APOPTOSIS; GENE THERAPY; GENES; IN VITRO; IN VIVO; IRRADIATION; LABELLING; LUNGS; MICE; NEOPLASMS; RADIOTHERAPY; TUMOR CELLS; VEINS

Citation Formats

Zeng Hui, Yuan Zhu, Zhu Hong, Li Lei, Shi Huashan, Wang Zi, Fan Yu, Deng Qian, Zeng Jianshuang, He Yinbo, Xiao Jianghong, and Li Zhiping, E-mail: lizhiping620312@yahoo.com.cn. Expression of hPNAS-4 Radiosensitizes Lewis Lung Cancer. United States: N. p., 2012. Web. doi:10.1016/J.IJROBP.2012.06.028.
Zeng Hui, Yuan Zhu, Zhu Hong, Li Lei, Shi Huashan, Wang Zi, Fan Yu, Deng Qian, Zeng Jianshuang, He Yinbo, Xiao Jianghong, & Li Zhiping, E-mail: lizhiping620312@yahoo.com.cn. Expression of hPNAS-4 Radiosensitizes Lewis Lung Cancer. United States. doi:10.1016/J.IJROBP.2012.06.028.
Zeng Hui, Yuan Zhu, Zhu Hong, Li Lei, Shi Huashan, Wang Zi, Fan Yu, Deng Qian, Zeng Jianshuang, He Yinbo, Xiao Jianghong, and Li Zhiping, E-mail: lizhiping620312@yahoo.com.cn. Thu . "Expression of hPNAS-4 Radiosensitizes Lewis Lung Cancer". United States. doi:10.1016/J.IJROBP.2012.06.028.
@article{osti_22149652,
title = {Expression of hPNAS-4 Radiosensitizes Lewis Lung Cancer},
author = {Zeng Hui and Yuan Zhu and Zhu Hong and Li Lei and Shi Huashan and Wang Zi and Fan Yu and Deng Qian and Zeng Jianshuang and He Yinbo and Xiao Jianghong and Li Zhiping, E-mail: lizhiping620312@yahoo.com.cn},
abstractNote = {Purpose: This study aimed to transfer the hPNAS-4 gene, a novel apoptosis-related human gene, into Lewis lung cancer (LL2) and observe its radiosensitive effect on radiation therapy in vitro and in vivo. Methods and Materials: The hPNAS-4 gene was transfected into LL2 cells, and its expression was detected via western blot. Colony formation assay and flow cytometry were used to detect the growth and apoptosis of cells treated with irradiation/PNAS-4 in vitro. The hPNAS-4 gene was transferred into LL2-bearing mice through tail vein injection of the liposome/gene complex. The tumor volumes were recorded after radiation therapy. Proliferating cell nuclear antigen (PCNA) immunohistochemistry staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were performed to detect the tumor cell growth and apoptosis in vivo. Results: The hPNAS-4 gene was successfully transferred into LL2 cells and tumor tissue, and its overexpressions were confirmed via western blot analysis. Compared with the control, empty plasmid, hPNAS-4, radiation, and empty plasmid plus radiation groups, the hPNAS-4 plus radiation group more significantly inhibited growth and enhanced apoptosis of LL2 cells in vitro and in vivo (P<.05). Conclusions: The hPNAS-4 gene was successfully transferred into LL2 cells and tumor tissue and was expressed in both LL2 cell and tumor tissue. The hPNAS-4 gene therapy significantly enhanced growth inhibition and apoptosis of LL2 tumor cells by radiation therapy in vitro and in vivo. Therefore, it may be a potential radiosensitive treatment of radiation therapy for lung cancer.},
doi = {10.1016/J.IJROBP.2012.06.028},
journal = {International Journal of Radiation Oncology, Biology and Physics},
issn = {0360-3016},
number = 4,
volume = 84,
place = {United States},
year = {2012},
month = {11}
}