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Title: Replication of murine coronavirus requires multiple cysteines in the endodomain of spike protein

Abstract

A conserved cysteine-rich motif located between the transmembrane domain and the endodomain is essential for membrane fusion and assembly of coronavirus spike (S) protein. Here, we proved that three cysteines within the motif, but not dependent on position, are minimally required for the survival of the recombinant mouse hepatitis virus. When the carboxy termini with these mutated motifs of S proteins were respectively introduced into a heterogeneous protein, both incorporation into lipid rafts and S-palmitoylation of these recombinant proteins showed a similar quantity requirement to cysteine residues. Meanwhile, the redistribution of these proteins on cellular surface indicated that the absence of the positively charged rather than cysteine residues in the motif might lead the dramatic reduction in syncytial formation of some mutants with the deleted motifs. These results suggest that multiple cysteine as well as charged residues concurrently improves the membrane-associated functions of S protein in viral replication and cytopathogenesis.

Authors:
; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22149274
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 427; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CYSTEINE; HEPATITIS; LEAD; LIPIDS; MICE; PROTEINS; VIRUSES

Citation Formats

Yang, Jinhua, Lv, Jun, Wang, Yuyan, Gao, Shuang, Yao, Qianqian, Qu, Di, and Ye, Rong, E-mail: yerong24@fudan.edu.cn. Replication of murine coronavirus requires multiple cysteines in the endodomain of spike protein. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.02.015.
Yang, Jinhua, Lv, Jun, Wang, Yuyan, Gao, Shuang, Yao, Qianqian, Qu, Di, & Ye, Rong, E-mail: yerong24@fudan.edu.cn. Replication of murine coronavirus requires multiple cysteines in the endodomain of spike protein. United States. doi:10.1016/J.VIROL.2012.02.015.
Yang, Jinhua, Lv, Jun, Wang, Yuyan, Gao, Shuang, Yao, Qianqian, Qu, Di, and Ye, Rong, E-mail: yerong24@fudan.edu.cn. Tue . "Replication of murine coronavirus requires multiple cysteines in the endodomain of spike protein". United States. doi:10.1016/J.VIROL.2012.02.015.
@article{osti_22149274,
title = {Replication of murine coronavirus requires multiple cysteines in the endodomain of spike protein},
author = {Yang, Jinhua and Lv, Jun and Wang, Yuyan and Gao, Shuang and Yao, Qianqian and Qu, Di and Ye, Rong, E-mail: yerong24@fudan.edu.cn},
abstractNote = {A conserved cysteine-rich motif located between the transmembrane domain and the endodomain is essential for membrane fusion and assembly of coronavirus spike (S) protein. Here, we proved that three cysteines within the motif, but not dependent on position, are minimally required for the survival of the recombinant mouse hepatitis virus. When the carboxy termini with these mutated motifs of S proteins were respectively introduced into a heterogeneous protein, both incorporation into lipid rafts and S-palmitoylation of these recombinant proteins showed a similar quantity requirement to cysteine residues. Meanwhile, the redistribution of these proteins on cellular surface indicated that the absence of the positively charged rather than cysteine residues in the motif might lead the dramatic reduction in syncytial formation of some mutants with the deleted motifs. These results suggest that multiple cysteine as well as charged residues concurrently improves the membrane-associated functions of S protein in viral replication and cytopathogenesis.},
doi = {10.1016/J.VIROL.2012.02.015},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 427,
place = {United States},
year = {2012},
month = {6}
}