Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells

doi:https://doi.org/10.1016/J.VIROL.2012.01.037

Title: Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells

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Abstract

The 2009 influenza A virus (IAV) pandemic resulted from reassortment of avian, human and swine strains probably in pigs. To elucidate the role of viral genes in host adaptation regarding innate immune responses, we focussed on the effect of genes from an avian H5N1 and a porcine H1N1 IAV on infectivity and activation of porcine GM-CSF-induced dendritic cells (DC). The highest interferon type I responses were achieved by the porcine virus reassortant containing the avian polymerase gene PB2. This finding was not due to differential tropism since all viruses infected DC equally. All viruses equally induced MHC class II, but porcine H1N1 expressing the avian viral PB2 induced more prominent nuclear NF-{kappa}B translocation compared to its parent IAV. The enhanced activation of DC may be detrimental or beneficial. An over-stimulation of innate responses could result in either pronounced tissue damage or increased resistance against IAV reassortants carrying avian PB2.

Authors:

Ocana-Macchi, Manuela; Ricklin, Meret E.; Python, Sylvie; Monika, Gsell-Albert ^[1]; Stech, Juergen; Stech, Olga ^[2]; Summerfield, Artur ^[1]

Institute of Virology and Immunoprophylaxis, Mittelhaeusern (Switzerland)
Friedrich-Loeffler Institut, Greifswald-Insel Riems (Germany)

Publication Date:: 2012-05-25

OSTI Identifier:: 22149272

Resource Type:: Journal Article

Journal Name:: Virology

Additional Journal Information:: Journal Volume: 427; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; DENDRITES; GENES; INFECTIVITY; INFLUENZA; INFLUENZA VIRUSES; INTERFERON; SWINE

Citation Formats


                    Ocana-Macchi, Manuela, Ricklin, Meret E., Python, Sylvie, Monika, Gsell-Albert, Stech, Juergen, Stech, Olga, and Summerfield, Artur. Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells.  United States: N. p., 2012. 
        Web.  doi:10.1016/J.VIROL.2012.01.037.

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                    Ocana-Macchi, Manuela, Ricklin, Meret E., Python, Sylvie, Monika, Gsell-Albert, Stech, Juergen, Stech, Olga, & Summerfield, Artur. Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells.  United States.  https://doi.org/10.1016/J.VIROL.2012.01.037

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                    Ocana-Macchi, Manuela, Ricklin, Meret E., Python, Sylvie, Monika, Gsell-Albert, Stech, Juergen, Stech, Olga, and Summerfield, Artur. Fri .  
        "Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells".  United States.  https://doi.org/10.1016/J.VIROL.2012.01.037.

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@article{osti_22149272,

  title        = {Avian influenza A virus PB2 promotes interferon type I inducing properties of a swine strain in porcine dendritic cells},

  author       = {Ocana-Macchi, Manuela and Ricklin, Meret E. and Python, Sylvie and Monika, Gsell-Albert and Stech, Juergen and Stech, Olga and Summerfield, Artur},

  abstractNote = {The 2009 influenza A virus (IAV) pandemic resulted from reassortment of avian, human and swine strains probably in pigs. To elucidate the role of viral genes in host adaptation regarding innate immune responses, we focussed on the effect of genes from an avian H5N1 and a porcine H1N1 IAV on infectivity and activation of porcine GM-CSF-induced dendritic cells (DC). The highest interferon type I responses were achieved by the porcine virus reassortant containing the avian polymerase gene PB2. This finding was not due to differential tropism since all viruses infected DC equally. All viruses equally induced MHC class II, but porcine H1N1 expressing the avian viral PB2 induced more prominent nuclear NF-{kappa}B translocation compared to its parent IAV. The enhanced activation of DC may be detrimental or beneficial. An over-stimulation of innate responses could result in either pronounced tissue damage or increased resistance against IAV reassortants carrying avian PB2.},

  doi          = {10.1016/J.VIROL.2012.01.037},

  url          = {https://www.osti.gov/biblio/22149272},
  journal      = {Virology},

  issn         = {0042-6822},

  number       = 1,

  volume       = 427,

  place        = {United States},

  year         = {2012},

  month        = {5}

}

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