skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells

Abstract

Picornavirus infection can lead to disruption of nuclear pore traffic, shut-off of cell translation machinery, and cleavage of proteins involved in cellular signal transduction and the innate response to infection. Here, we demonstrated that the FMDV 3C{sup pro} induced the cleavage of nuclear RNA-binding protein Sam68 C-terminus containing the nuclear localization sequence (NLS). Consequently, it stimulated the redistribution of Sam68 to the cytoplasm. The siRNA knockdown of Sam68 resulted in a 1000-fold reduction in viral titers, which prompted us to study the effect of Sam68 on FMDV post-entry events. Interestingly, Sam68 interacts with the internal ribosomal entry site within the 5 Prime non-translated region of the FMDV genome, and Sam68 knockdown decreased FMDV IRES-driven activity in vitro suggesting that it could modulate translation of the viral genome. The results uncover a novel role for Sam68 in the context of picornaviruses and the proteolysis of a new cellular target of the FMDV 3C{sup pro}.

Authors:
; ;
Publication Date:
OSTI Identifier:
22149264
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 425; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CATTLE; CLEAVAGE; CYTOPLASM; IN VITRO; PROTEINS; PROTEOLYSIS; RNA; VIRAL DISEASES; VIRUSES

Citation Formats

Lawrence, Paul, Schafer, Elizabeth A., and Rieder, Elizabeth, E-mail: elizabeth.rieder@ars.usda.gov. The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2011.12.019.
Lawrence, Paul, Schafer, Elizabeth A., & Rieder, Elizabeth, E-mail: elizabeth.rieder@ars.usda.gov. The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells. United States. doi:10.1016/J.VIROL.2011.12.019.
Lawrence, Paul, Schafer, Elizabeth A., and Rieder, Elizabeth, E-mail: elizabeth.rieder@ars.usda.gov. Fri . "The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells". United States. doi:10.1016/J.VIROL.2011.12.019.
@article{osti_22149264,
title = {The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells},
author = {Lawrence, Paul and Schafer, Elizabeth A. and Rieder, Elizabeth, E-mail: elizabeth.rieder@ars.usda.gov},
abstractNote = {Picornavirus infection can lead to disruption of nuclear pore traffic, shut-off of cell translation machinery, and cleavage of proteins involved in cellular signal transduction and the innate response to infection. Here, we demonstrated that the FMDV 3C{sup pro} induced the cleavage of nuclear RNA-binding protein Sam68 C-terminus containing the nuclear localization sequence (NLS). Consequently, it stimulated the redistribution of Sam68 to the cytoplasm. The siRNA knockdown of Sam68 resulted in a 1000-fold reduction in viral titers, which prompted us to study the effect of Sam68 on FMDV post-entry events. Interestingly, Sam68 interacts with the internal ribosomal entry site within the 5 Prime non-translated region of the FMDV genome, and Sam68 knockdown decreased FMDV IRES-driven activity in vitro suggesting that it could modulate translation of the viral genome. The results uncover a novel role for Sam68 in the context of picornaviruses and the proteolysis of a new cellular target of the FMDV 3C{sup pro}.},
doi = {10.1016/J.VIROL.2011.12.019},
journal = {Virology},
number = 1,
volume = 425,
place = {United States},
year = {Fri Mar 30 00:00:00 EDT 2012},
month = {Fri Mar 30 00:00:00 EDT 2012}
}