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Title: Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source

Abstract

We developed an off-axis-illuminated zone-plate-based hard x-ray Zernike phase-contrast microscope beamline at Pohang Light Source. Owing to condenser optics-free and off-axis illumination, a large field of view was achieved. The pinhole-type Zernike phase plate affords high-contrast images of a cell with minimal artifacts such as the shade-off and halo effects. The setup, including the optics and the alignment, is simple and easy, and allows faster and easier imaging of large bio-samples.

Authors:
;  [1];  [2]; ;  [3]
  1. Pohang Accelerator Laboratory, POSTECH, Pohang 790-784 (Korea, Republic of)
  2. Department of Physics, POSTECH, Pohang 790-784 (Korea, Republic of)
  3. Department of Anatomy, Catholic University of Daegu, Daegu 705-034 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22105362
Resource Type:
Journal Article
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 84; Journal Issue: 1; Other Information: (c) 2013 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0034-6748
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; HARD X RADIATION; LIGHT SOURCES; MICROSCOPY; PLATES; POHANG LIGHT SOURCE

Citation Formats

Lim, Jun, Huang, Jung Yun, Park, So Yeong, Han, Sung Mi, and Kim, Hong-Tae. Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source. United States: N. p., 2013. Web. doi:10.1063/1.4789362.
Lim, Jun, Huang, Jung Yun, Park, So Yeong, Han, Sung Mi, & Kim, Hong-Tae. Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source. United States. https://doi.org/10.1063/1.4789362
Lim, Jun, Huang, Jung Yun, Park, So Yeong, Han, Sung Mi, and Kim, Hong-Tae. 2013. "Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source". United States. https://doi.org/10.1063/1.4789362.
@article{osti_22105362,
title = {Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source},
author = {Lim, Jun and Huang, Jung Yun and Park, So Yeong and Han, Sung Mi and Kim, Hong-Tae},
abstractNote = {We developed an off-axis-illuminated zone-plate-based hard x-ray Zernike phase-contrast microscope beamline at Pohang Light Source. Owing to condenser optics-free and off-axis illumination, a large field of view was achieved. The pinhole-type Zernike phase plate affords high-contrast images of a cell with minimal artifacts such as the shade-off and halo effects. The setup, including the optics and the alignment, is simple and easy, and allows faster and easier imaging of large bio-samples.},
doi = {10.1063/1.4789362},
url = {https://www.osti.gov/biblio/22105362}, journal = {Review of Scientific Instruments},
issn = {0034-6748},
number = 1,
volume = 84,
place = {United States},
year = {Tue Jan 15 00:00:00 EST 2013},
month = {Tue Jan 15 00:00:00 EST 2013}
}