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Title: Protein immobilization and detection on laser processed polystyrene surfaces

Abstract

The bovine serum albumin (BSA)-polystyrene (PS) interface layer is laser photo activated at 157 nm for site selective multiple target-protein immobilization. The 5-15 nm photon induced interface layer has different chemical, wetting, and stiffness properties than the PS photon processed surface. The irradiated areas exhibit target-protein binding, followed by localized probe-target protein detection. The photon induced chemical modification of the BSA-PS interface layer is identified by: (1) Morphological, imaging, and analysis of surface parameters with atomic force microscopy, (2) spectroscopic shift (4 cm{sup -1}), of the amide I group and formation of new C=N, NH{sub 2}, C-O, C=O, and O-C=O groups following irradiation, identified with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and (3) the different hydrophilic/hydrophobic and force-distance response of the bare PS and BSA-PS surfaces. Near field edge diffraction (Fresnel) fluorescence imaging specifies the threshold photon energy and the fluence required to optically detect the protein binding on the photon induced BSA-PS interface layer. By approximating the Fresnel integrals with analytical functions, the threshold photon energy and the fluence are expressed as the sum of zero, first, and second order harmonic terms of two characteristic diffracted modes and they are specified to be 8.73x10{sup -9} Jand623 Jmore » m{sup -2}, respectively. Furthermore, a bioarray of three probe-target proteins is fabricated with 1.5 {mu}m spatial resolution using a 157 nm laser microstepper. The methodology eliminates the use of intermediate polymer layers between the blocking BSA protein and the PS substrate in bioarray fabrication.« less

Authors:
; ; ; ;  [1]; ;  [2]
  1. National Hellenic Research Foundation, Theoretical and Physical Chemistry Institute, 48 Vassileos Constantinou Avenue, Athens 11635 (Greece)
  2. N.C.S.R. ''Demokritos'', Institute of Radioisotopes and Radiodiagnostic Products, Immunoassay/Immunosensors Lab., Patriarchou Gregoriou Str, Aghia Paraskevi, Athens 15310 (Greece)
Publication Date:
OSTI Identifier:
22036727
Resource Type:
Journal Article
Journal Name:
Journal of Applied Physics
Additional Journal Information:
Journal Volume: 110; Journal Issue: 6; Other Information: (c) 2011 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0021-8979
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 60 APPLIED LIFE SCIENCES; ALBUMINS; ATOMIC FORCE MICROSCOPY; BIOCHEMISTRY; CATTLE; CHANNELING; DIFFRACTION; FLUORESCENCE; FOURIER TRANSFORMATION; FUNCTIONS; INFRARED SPECTRA; INTERFACES; IRRADIATION; LAYERS; PHOTOCHEMISTRY; POLYSTYRENE; RADIATION EFFECTS; SPATIAL RESOLUTION; SPECTROSCOPY; SUBSTRATES; SURFACES

Citation Formats

Sarantopoulou, Evangelia, Kollia, Zoe, Palles, Dimitrios, Spyropoulos-Antonakakis, Nikolaos, Cefalas, Alkiviadis-Constantinos, Petrou, Panagiota S., and Kakabakos, Sotirios. Protein immobilization and detection on laser processed polystyrene surfaces. United States: N. p., 2011. Web. doi:10.1063/1.3627160.
Sarantopoulou, Evangelia, Kollia, Zoe, Palles, Dimitrios, Spyropoulos-Antonakakis, Nikolaos, Cefalas, Alkiviadis-Constantinos, Petrou, Panagiota S., & Kakabakos, Sotirios. Protein immobilization and detection on laser processed polystyrene surfaces. United States. doi:10.1063/1.3627160.
Sarantopoulou, Evangelia, Kollia, Zoe, Palles, Dimitrios, Spyropoulos-Antonakakis, Nikolaos, Cefalas, Alkiviadis-Constantinos, Petrou, Panagiota S., and Kakabakos, Sotirios. Thu . "Protein immobilization and detection on laser processed polystyrene surfaces". United States. doi:10.1063/1.3627160.
@article{osti_22036727,
title = {Protein immobilization and detection on laser processed polystyrene surfaces},
author = {Sarantopoulou, Evangelia and Kollia, Zoe and Palles, Dimitrios and Spyropoulos-Antonakakis, Nikolaos and Cefalas, Alkiviadis-Constantinos and Petrou, Panagiota S. and Kakabakos, Sotirios},
abstractNote = {The bovine serum albumin (BSA)-polystyrene (PS) interface layer is laser photo activated at 157 nm for site selective multiple target-protein immobilization. The 5-15 nm photon induced interface layer has different chemical, wetting, and stiffness properties than the PS photon processed surface. The irradiated areas exhibit target-protein binding, followed by localized probe-target protein detection. The photon induced chemical modification of the BSA-PS interface layer is identified by: (1) Morphological, imaging, and analysis of surface parameters with atomic force microscopy, (2) spectroscopic shift (4 cm{sup -1}), of the amide I group and formation of new C=N, NH{sub 2}, C-O, C=O, and O-C=O groups following irradiation, identified with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and (3) the different hydrophilic/hydrophobic and force-distance response of the bare PS and BSA-PS surfaces. Near field edge diffraction (Fresnel) fluorescence imaging specifies the threshold photon energy and the fluence required to optically detect the protein binding on the photon induced BSA-PS interface layer. By approximating the Fresnel integrals with analytical functions, the threshold photon energy and the fluence are expressed as the sum of zero, first, and second order harmonic terms of two characteristic diffracted modes and they are specified to be 8.73x10{sup -9} Jand623 J m{sup -2}, respectively. Furthermore, a bioarray of three probe-target proteins is fabricated with 1.5 {mu}m spatial resolution using a 157 nm laser microstepper. The methodology eliminates the use of intermediate polymer layers between the blocking BSA protein and the PS substrate in bioarray fabrication.},
doi = {10.1063/1.3627160},
journal = {Journal of Applied Physics},
issn = {0021-8979},
number = 6,
volume = 110,
place = {United States},
year = {2011},
month = {9}
}