skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Escape from R-peptide deletion in a {gamma}-retrovirus

Abstract

The R peptide in the cytoplasmic tail (C-tail) of {gamma}-retroviral envelope proteins (Env) prevents membrane fusion before budding. To analyse its role in the formation of replication competent, infectious particles, we developed chimeric murine leukaemia viruses (MLV) with unmodified or R-peptide deleted Env proteins of the gibbon ape leukaemia virus (GaLV). While titres of these viruses were unaffected, R-peptide deficiency led to strongly impaired spreading. Most remarkably, we isolated an escape mutant which had restored an open reading frame for a C-terminal extension of the truncated C-tail. A reconstituted virus encoding this escape C-tail replicated in cell culture. In contrast to R-peptide deficient Env, particle incorporation of the escape Env was effective due to an enhanced protein expression and restored intracellular co-localisation with Gag proteins. Our data demonstrate that the R peptide not only regulates membrane fusion but also mediates efficient Env protein particle incorporation in {gamma}-retrovirus infected cells.

Authors:
; ;  [1];  [2];  [1];  [1]
  1. Division of Medical Biotechnology, Paul-Ehrlich-Institut, 63225 Langen (Germany)
  2. Department of Immunology and Molecular Pathology, University College London (United Kingdom)
Publication Date:
OSTI Identifier:
21587892
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 418; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2011.07.011; PII: S0042-6822(11)00317-5; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL CULTURES; LEUKEMIA; MEMBRANES; MUTANTS; PARTICLES; PEPTIDES; VIRUSES; DISEASES; IMMUNE SYSTEM DISEASES; MICROORGANISMS; NEOPLASMS; ORGANIC COMPOUNDS; PARASITES; PROTEINS

Citation Formats

Schneider, Irene C., Eckhardt, Manon, Brynza, Julia, Collins, Mary K., Cichutek, Klaus, and Buchholz, Christian J., E-mail: christian.buchholz@pei.de. Escape from R-peptide deletion in a {gamma}-retrovirus. United States: N. p., 2011. Web. doi:10.1016/j.virol.2011.07.011.
Schneider, Irene C., Eckhardt, Manon, Brynza, Julia, Collins, Mary K., Cichutek, Klaus, & Buchholz, Christian J., E-mail: christian.buchholz@pei.de. Escape from R-peptide deletion in a {gamma}-retrovirus. United States. doi:10.1016/j.virol.2011.07.011.
Schneider, Irene C., Eckhardt, Manon, Brynza, Julia, Collins, Mary K., Cichutek, Klaus, and Buchholz, Christian J., E-mail: christian.buchholz@pei.de. Fri . "Escape from R-peptide deletion in a {gamma}-retrovirus". United States. doi:10.1016/j.virol.2011.07.011.
@article{osti_21587892,
title = {Escape from R-peptide deletion in a {gamma}-retrovirus},
author = {Schneider, Irene C. and Eckhardt, Manon and Brynza, Julia and Collins, Mary K. and Cichutek, Klaus and Buchholz, Christian J., E-mail: christian.buchholz@pei.de},
abstractNote = {The R peptide in the cytoplasmic tail (C-tail) of {gamma}-retroviral envelope proteins (Env) prevents membrane fusion before budding. To analyse its role in the formation of replication competent, infectious particles, we developed chimeric murine leukaemia viruses (MLV) with unmodified or R-peptide deleted Env proteins of the gibbon ape leukaemia virus (GaLV). While titres of these viruses were unaffected, R-peptide deficiency led to strongly impaired spreading. Most remarkably, we isolated an escape mutant which had restored an open reading frame for a C-terminal extension of the truncated C-tail. A reconstituted virus encoding this escape C-tail replicated in cell culture. In contrast to R-peptide deficient Env, particle incorporation of the escape Env was effective due to an enhanced protein expression and restored intracellular co-localisation with Gag proteins. Our data demonstrate that the R peptide not only regulates membrane fusion but also mediates efficient Env protein particle incorporation in {gamma}-retrovirus infected cells.},
doi = {10.1016/j.virol.2011.07.011},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 418,
place = {United States},
year = {2011},
month = {9}
}