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Title: Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)

Abstract

Contamination of animal feed with mycotoxins is a major problem for fish feed mainly due to usage of contaminated ingredients for production and inappropriate storage of feed. The use of cereals for fish food production further increases the risk of a potential contamination. Potential contaminants include the mycotoxin deoxynivalenol (DON) which is synthesized by globally distributed fungi of the genus Fusarium. The toxicity of DON is well recognized in mammals. In this study, we confirm cytotoxic effects of DON in established permanent fish cell lines. We demonstrate that DON is capable of influencing the metabolic activity and cell viability in fish cells as determined by different assays to indicate possible cellular targets of this toxin. Evaluation of cell viability by measurement of membrane integrity, mitochondrial activity and lysosomal function after 24 h of exposure of fish cell lines to DON at a concentration range of 0-3000 ng ml{sup -1} shows a biphasic effect on cells although differences in sensitivity occur. The cell lines derived from rainbow trout are particularly sensitive to DON. The focus of this study lies, furthermore, on the effects of DON at different concentrations on production of reactive oxygen species (ROS) in the different fish cell lines.more » The results show that DON mainly reduces ROS production in all cell lines that were used. Thus, our comparative investigations reveal that the fish cell lines show distinct species-related endpoint sensitivities that also depend on the type of tissue from which the cells were derived and the severity of exposure. - Highlights: > DON uptake by cells is not extensive. > All fish cell lines are sensitive to DON. > DON is most cytotoxic to rainbow trout cells. > Biphasic cellular responses were frequently observed. > Our results are similar to studies on mammalian cell lines.« less

Authors:
 [1]; ;  [2];  [1]
  1. University Basel, Man-Society-Environment, Department of Environmental Sciences, Vesalgasse 1, CH-4051 Basel (Switzerland)
  2. Agroscope Reckenholz-Taenikon (ART), Research Station ART, Reckenholzstrasse 191, CH-8046 Zuerich (Switzerland)
Publication Date:
OSTI Identifier:
21587848
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 256; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2011.07.004; PII: S0041-008X(11)00261-4; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL FEEDS; CELL CULTURES; CONTAMINATION; FUSARIUM; MAMMALS; MYCOTOXINS; SENSITIVITY; TOXICITY; TROUT; VIABILITY; ANIMALS; ANTIGENS; AQUATIC ORGANISMS; EUMYCOTA; FISHES; FOOD; FUNGI; HAZARDOUS MATERIALS; MATERIALS; PARASITES; PLANTS; TOXIC MATERIALS; TOXINS; VERTEBRATES

Citation Formats

Pietsch, Constanze, E-mail: constanze.pietsch@unibas.ch, Bucheli, Thomas D., Wettstein, Felix E., and Burkhardt-Holm, Patricia. Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON). United States: N. p., 2011. Web. doi:10.1016/j.taap.2011.07.004.
Pietsch, Constanze, E-mail: constanze.pietsch@unibas.ch, Bucheli, Thomas D., Wettstein, Felix E., & Burkhardt-Holm, Patricia. Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON). United States. doi:10.1016/j.taap.2011.07.004.
Pietsch, Constanze, E-mail: constanze.pietsch@unibas.ch, Bucheli, Thomas D., Wettstein, Felix E., and Burkhardt-Holm, Patricia. Sat . "Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)". United States. doi:10.1016/j.taap.2011.07.004.
@article{osti_21587848,
title = {Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)},
author = {Pietsch, Constanze, E-mail: constanze.pietsch@unibas.ch and Bucheli, Thomas D. and Wettstein, Felix E. and Burkhardt-Holm, Patricia},
abstractNote = {Contamination of animal feed with mycotoxins is a major problem for fish feed mainly due to usage of contaminated ingredients for production and inappropriate storage of feed. The use of cereals for fish food production further increases the risk of a potential contamination. Potential contaminants include the mycotoxin deoxynivalenol (DON) which is synthesized by globally distributed fungi of the genus Fusarium. The toxicity of DON is well recognized in mammals. In this study, we confirm cytotoxic effects of DON in established permanent fish cell lines. We demonstrate that DON is capable of influencing the metabolic activity and cell viability in fish cells as determined by different assays to indicate possible cellular targets of this toxin. Evaluation of cell viability by measurement of membrane integrity, mitochondrial activity and lysosomal function after 24 h of exposure of fish cell lines to DON at a concentration range of 0-3000 ng ml{sup -1} shows a biphasic effect on cells although differences in sensitivity occur. The cell lines derived from rainbow trout are particularly sensitive to DON. The focus of this study lies, furthermore, on the effects of DON at different concentrations on production of reactive oxygen species (ROS) in the different fish cell lines. The results show that DON mainly reduces ROS production in all cell lines that were used. Thus, our comparative investigations reveal that the fish cell lines show distinct species-related endpoint sensitivities that also depend on the type of tissue from which the cells were derived and the severity of exposure. - Highlights: > DON uptake by cells is not extensive. > All fish cell lines are sensitive to DON. > DON is most cytotoxic to rainbow trout cells. > Biphasic cellular responses were frequently observed. > Our results are similar to studies on mammalian cell lines.},
doi = {10.1016/j.taap.2011.07.004},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 256,
place = {United States},
year = {2011},
month = {10}
}