Multiplexed biomarker detection using x-ray fluorescence of composition-encoded nanoparticles

Journal Article · Mon Dec 27 00:00:00 EST 2010 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.3533817· OSTI ID:21518229

Hossain, Mainul; Chaoming, Wang; Ming, Su ^[1]

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1. NanoScience Technology Center, University of Central Florida, Orlando, Florida 32826 (United States)

Multiple DNA and protein biomarkers have been detected based on characteristic x-ray fluorescence of a panel of metal and alloy nanoparticles, which are modified with ligands of biomarkers to create a one-to-one correspondence and immobilized on ligand-modified substrates after forming complexes with target biomarkers in three-strand or sandwich configuration. By determining the presence and concentration of nanoparticles using x-ray fluorescence, the nature and amount of biomarkers can be detected with limits of 1 nM for DNA and 1 ng/ml for protein. By combining high penetrating ability of x-rays, this method allows quantitative imaging of multiple biomarkers.

• OSTI ID: 21518229
• Journal Information: Applied Physics Letters, Vol. 97, Issue 26; Other Information: DOI: 10.1063/1.3533817; (c) 2010 American Institute of Physics; ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ALLOYS
BIOLOGICAL MARKERS
BIOPHYSICS
CONFIGURATION
DETECTION
DNA
EMISSION SPECTRA
FLUORESCENCE
LIGANDS
MATERIALS
METALS
NANOSTRUCTURES
PARTICLES
PROTEINS
SUBSTRATES
X RADIATION
X-RAY FLUORESCENCE ANALYSIS
X-RAY SPECTRA
CHEMICAL ANALYSIS
ELECTROMAGNETIC RADIATION
ELEMENTS
EMISSION
IONIZING RADIATIONS
LUMINESCENCE
NONDESTRUCTIVE ANALYSIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTON EMISSION
PHYSICS
RADIATIONS
SPECTRA
X-RAY EMISSION ANALYSIS