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Title: Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes

Abstract

It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72 h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-{sub L}-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.

Authors:
; ; ; ;  [1];  [2];  [1]
  1. Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan (China)
  2. Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan (China)
Publication Date:
OSTI Identifier:
21460193
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 246; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2010.05.003; PII: S0041-008X(10)00167-5; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; DOXORUBICIN; GLUTATHIONE; MONOCYTES; OXYGENASES; PROTOPORPHYRINS; PSYCHOTROPIC DRUGS; ANTIBIOTICS; ANTI-INFECTIVE AGENTS; ANTINEOPLASTIC DRUGS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM AGENTS; DRUGS; ENZYMES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; LEUKOCYTES; MATERIALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDOREDUCTASES; PEPTIDES; PIGMENTS; POLYPEPTIDES; PORPHYRINS; PROTEINS; RADIOPROTECTIVE SUBSTANCES; RESPONSE MODIFYING FACTORS

Citation Formats

Wu, Hsin-Ying, Chang, An-Chi, Wang, Chia-Chi, Kuo, Fu-Hua, Lee, Chi-Ya, Liu, Der-Zen, and Jan, Tong-Rong, E-mail: tonyjan@ntu.edu.t. Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. United States: N. p., 2010. Web. doi:10.1016/j.taap.2010.05.003.
Wu, Hsin-Ying, Chang, An-Chi, Wang, Chia-Chi, Kuo, Fu-Hua, Lee, Chi-Ya, Liu, Der-Zen, & Jan, Tong-Rong, E-mail: tonyjan@ntu.edu.t. Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. United States. doi:10.1016/j.taap.2010.05.003.
Wu, Hsin-Ying, Chang, An-Chi, Wang, Chia-Chi, Kuo, Fu-Hua, Lee, Chi-Ya, Liu, Der-Zen, and Jan, Tong-Rong, E-mail: tonyjan@ntu.edu.t. Sun . "Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes". United States. doi:10.1016/j.taap.2010.05.003.
@article{osti_21460193,
title = {Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes},
author = {Wu, Hsin-Ying and Chang, An-Chi and Wang, Chia-Chi and Kuo, Fu-Hua and Lee, Chi-Ya and Liu, Der-Zen and Jan, Tong-Rong, E-mail: tonyjan@ntu.edu.t},
abstractNote = {It has been documented that cannabidiol (CBD) induced apoptosis in a variety of transformed cells, including lymphocytic and monocytic leukemias. In contrast, a differential sensitivity between normal lymphocytes and monocytes to CBD-mediated apoptosis has been reported. The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72 h. CBD markedly enhanced apoptosis of freshly isolated monocytes in a time- and concentration-dependent manner, whereas precultured monocytes were insensitive. By comparison, both cells were sensitive to doxorubicin-induced apoptosis. CBD significantly diminished the cellular thiols and glutathione in freshly isolated monocytes. The apoptosis induced by CBD was abrogated in the presence of N-acetyl-{sub L}-cysteine, a precursor of glutathione. In addition, precultured monocytes contained a significantly greater level of glutathione and heme oxygenase-1 (HO-1) compared to the freshly isolated cells. The HO-1 competitive inhibitor zinc protoporphyrin partially but significantly restored the sensitivity of precultured monocytes to CBD-mediated apoptosis. Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells.},
doi = {10.1016/j.taap.2010.05.003},
journal = {Toxicology and Applied Pharmacology},
number = 3,
volume = 246,
place = {United States},
year = {Sun Aug 01 00:00:00 EDT 2010},
month = {Sun Aug 01 00:00:00 EDT 2010}
}