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Title: Optical clearing of the eye sclera in vivo caused by glucose

Abstract

Changes in the optical properties of the eye sclera of a laboratory animal (rabbit) caused by the noninvasive administration of an immersion agent (aqueous glucose solution) into sclera are studied experimentally in vivo by the method of reflection spectroscopy and simulated numerically. The diffusion coefficients of glucose and water in sclera are estimated from simulations of optical clearing within the framework of the developed model as h(5.4{+-}0.1)x10{sup -7} and (5.8{+-}0.2)x10{sup -7} cm{sup 2} s{sup -1}, respectively. Experiments showed that the application of the glucose solution on the sclera allows the efficient control of its optical properties, which makes it possible to deliver the laser energy to internal eye tissues without considerable attenuation and distortions of its spatial distribution in laser diagnostics and therapy. (special issue devoted to multiple radiation scattering in random media)

Authors:
; ; ;  [1]
  1. Department of Optics and Biomedical Physics, N.G.Chernyshevskii Saratov State University (Russian Federation)
Publication Date:
OSTI Identifier:
21456918
Resource Type:
Journal Article
Resource Relation:
Journal Name: Quantum Electronics (Woodbury, N.Y.); Journal Volume: 36; Journal Issue: 12; Other Information: DOI: 10.1070/QE2006v036n12ABEH013337
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AQUEOUS SOLUTIONS; DIAGNOSTIC TECHNIQUES; DIFFUSION; EYES; GLUCOSE; IN VIVO; LABORATORY ANIMALS; LASERS; OPTICAL PROPERTIES; RABBITS; REFLECTION; SIMULATION; SPATIAL DISTRIBUTION; SPECTROSCOPY; THERAPY; ALDEHYDES; ANIMALS; BODY; CARBOHYDRATES; DISPERSIONS; DISTRIBUTION; FACE; HEAD; HEXOSES; HOMOGENEOUS MIXTURES; MAMMALS; MEDICINE; MIXTURES; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; PHYSICAL PROPERTIES; SACCHARIDES; SENSE ORGANS; SOLUTIONS; VERTEBRATES

Citation Formats

Genina, Elina A, Bashkatov, A N, Sinichkin, Yu P, and Tuchin, Valerii V. Optical clearing of the eye sclera in vivo caused by glucose. United States: N. p., 2006. Web. doi:10.1070/QE2006V036N12ABEH013337.
Genina, Elina A, Bashkatov, A N, Sinichkin, Yu P, & Tuchin, Valerii V. Optical clearing of the eye sclera in vivo caused by glucose. United States. doi:10.1070/QE2006V036N12ABEH013337.
Genina, Elina A, Bashkatov, A N, Sinichkin, Yu P, and Tuchin, Valerii V. Sun . "Optical clearing of the eye sclera in vivo caused by glucose". United States. doi:10.1070/QE2006V036N12ABEH013337.
@article{osti_21456918,
title = {Optical clearing of the eye sclera in vivo caused by glucose},
author = {Genina, Elina A and Bashkatov, A N and Sinichkin, Yu P and Tuchin, Valerii V},
abstractNote = {Changes in the optical properties of the eye sclera of a laboratory animal (rabbit) caused by the noninvasive administration of an immersion agent (aqueous glucose solution) into sclera are studied experimentally in vivo by the method of reflection spectroscopy and simulated numerically. The diffusion coefficients of glucose and water in sclera are estimated from simulations of optical clearing within the framework of the developed model as h(5.4{+-}0.1)x10{sup -7} and (5.8{+-}0.2)x10{sup -7} cm{sup 2} s{sup -1}, respectively. Experiments showed that the application of the glucose solution on the sclera allows the efficient control of its optical properties, which makes it possible to deliver the laser energy to internal eye tissues without considerable attenuation and distortions of its spatial distribution in laser diagnostics and therapy. (special issue devoted to multiple radiation scattering in random media)},
doi = {10.1070/QE2006V036N12ABEH013337},
journal = {Quantum Electronics (Woodbury, N.Y.)},
number = 12,
volume = 36,
place = {United States},
year = {Sun Dec 31 00:00:00 EST 2006},
month = {Sun Dec 31 00:00:00 EST 2006}
}
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  • Investigation of the diffusion of cytamines, a typical representative of which is cortexin, is important for evaluating the drug dose, necessary to provide sufficient concentration of the preparation in the inner tissues of the eye. In the present paper, the cortexin diffusion rate in the eye sclera is measured using the methods of optical coherence tomography (OCT) and reflectance spectroscopy. The technique for determining the diffusion coefficient is based on the registration of temporal dependence of the eye sclera scattering parameters caused by partial replacement of interstitial fluid with the aqueous cortexin solution, which reduces the level of the OCTmore » signal and decreases the reflectance of the sclera. The values of the cortexin diffusion coefficient obtained using two independent optical methods are in good agreement. (optical technologies in biophysics and medicine)« less
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  • The spectral dependence of the optical properties of human sclera adjacent to the limbus was investigated and related to the potentials of transscleral photocoagulation. The total transmission, absorption, and reflection, as well as the angular distribution of the transmitted and reflected light were measured at five laser wavelengths (442 nm, 514 nm, 633 nm, 804 nm, and 1,064 nm), both for noncontact and contact applications. Absorption and scattering coefficients were determined using the Kubelka-Munk model for light propagation through a scattering tissue. The scleral transmission is only 6% at 442 nm but increases to 35% at 804 nm and tomore » 53% at 1,064 nm. The absorption is high at short wavelengths with 40% at 442 nm but it is only 6% at 804 nm and 1,064 nm. The reflection is generally higher than 40% and shows little wavelength dependence. The transmitted light is scattered diffusely at short wavelengths, but at 804 nm and 1,064 nm it exhibits a fairly narrow angular distribution in forward direction. Fiber contact leads to an increase of transmission, with a factor of 3.5 at 442 nm, of 2.0 at 804 nm, and 1.5 at 1,064 nm. Our results indicate that the diode laser (804 nm) and the Nd:YAG laser (1,064 nm) with contact delivery are best suited for transscleral photocoagulation.« less
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