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Title: Saturation fluorimetry of complex organic compounds with a high local concentration of fluorophores (by the example of phytoplankton)

Abstract

Saturation of fluorescence of complex organic compounds with a high local concentration of fluorescing molecules (fluorophores), when singlet-singlet annihilation makes a noticeable contribution to saturation, is considered. The fluorescence saturation curve is obtained analytically for the case of a rectangular temporal and spatial distribution of photons in a laser pulse. It is shown that the fluorescence saturation curve depends on the parameter {Phi}{sub 0}, which is proportional to the concentration of fluorescing molecules, and on the parameters A, B, and {alpha} describing the influence of singlet-singlet annihilation, bleaching of an optically thin layer, and nonstationarity of excitation, respectively. The fluorescence saturation curves are studied experimentally for compounds with a high local concentration of fluorescing molecules such as molecules of a monoculture of diatomic alga Thalassiosira weissflogii. The experimental fluorescence saturation curves are well described by the obtained analytic expression. The values of the parameter {Phi}{sub 0}, proportional to the concentration of chlorophyll a, and the parameter A (for the first time) are obtained from the alga fluorescence saturation curves. (laser applications and other topics in quantum electronics)

Authors:
; ;  [1]
  1. Department of Physics, M.V. Lomonosov Moscow State University, Moscow (Russian Federation)
Publication Date:
OSTI Identifier:
21456846
Resource Type:
Journal Article
Resource Relation:
Journal Name: Quantum Electronics (Woodbury, N.Y.); Journal Volume: 36; Journal Issue: 2; Other Information: DOI: 10.1070/QE2006v036n02ABEH012791
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ALGAE; ANNIHILATION; CHLOROPHYLL; DIAGRAMS; EXCITATION; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; LASERS; MOLECULES; PHOTONS; PHYTOPLANKTON; SPATIAL DISTRIBUTION; THIN FILMS; AQUATIC ORGANISMS; BOSONS; CARBOXYLIC ACIDS; DISTRIBUTION; ELEMENTARY PARTICLES; EMISSION; EMISSION SPECTROSCOPY; ENERGY-LEVEL TRANSITIONS; FILMS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; INFORMATION; INTERACTIONS; LUMINESCENCE; MASSLESS PARTICLES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARTICLE INTERACTIONS; PHOTON EMISSION; PHYTOCHROMES; PIGMENTS; PLANKTON; PLANTS; PORPHYRINS; PROTEINS; SPECTROSCOPY

Citation Formats

Maslov, D V, Ostroumov, E E, and Fadeev, V V. Saturation fluorimetry of complex organic compounds with a high local concentration of fluorophores (by the example of phytoplankton). United States: N. p., 2006. Web. doi:10.1070/QE2006V036N02ABEH012791.
Maslov, D V, Ostroumov, E E, & Fadeev, V V. Saturation fluorimetry of complex organic compounds with a high local concentration of fluorophores (by the example of phytoplankton). United States. doi:10.1070/QE2006V036N02ABEH012791.
Maslov, D V, Ostroumov, E E, and Fadeev, V V. Tue . "Saturation fluorimetry of complex organic compounds with a high local concentration of fluorophores (by the example of phytoplankton)". United States. doi:10.1070/QE2006V036N02ABEH012791.
@article{osti_21456846,
title = {Saturation fluorimetry of complex organic compounds with a high local concentration of fluorophores (by the example of phytoplankton)},
author = {Maslov, D V and Ostroumov, E E and Fadeev, V V},
abstractNote = {Saturation of fluorescence of complex organic compounds with a high local concentration of fluorescing molecules (fluorophores), when singlet-singlet annihilation makes a noticeable contribution to saturation, is considered. The fluorescence saturation curve is obtained analytically for the case of a rectangular temporal and spatial distribution of photons in a laser pulse. It is shown that the fluorescence saturation curve depends on the parameter {Phi}{sub 0}, which is proportional to the concentration of fluorescing molecules, and on the parameters A, B, and {alpha} describing the influence of singlet-singlet annihilation, bleaching of an optically thin layer, and nonstationarity of excitation, respectively. The fluorescence saturation curves are studied experimentally for compounds with a high local concentration of fluorescing molecules such as molecules of a monoculture of diatomic alga Thalassiosira weissflogii. The experimental fluorescence saturation curves are well described by the obtained analytic expression. The values of the parameter {Phi}{sub 0}, proportional to the concentration of chlorophyll a, and the parameter A (for the first time) are obtained from the alga fluorescence saturation curves. (laser applications and other topics in quantum electronics)},
doi = {10.1070/QE2006V036N02ABEH012791},
journal = {Quantum Electronics (Woodbury, N.Y.)},
number = 2,
volume = 36,
place = {United States},
year = {Tue Feb 28 00:00:00 EST 2006},
month = {Tue Feb 28 00:00:00 EST 2006}
}