Modulation of DNA repair capacity and mRNA expression levels of XRCC1, hOGG1 and XPC genes in styrene-exposed workers

Journal Article · Mon Nov 01 00:00:00 EDT 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2010.07.027· OSTI ID:21451191

Hanova, Monika ^[1]; Stetina, Rudolf ^[2]; Vodickova, Ludmila ^[1]; Vaclavikova, Radka ^[3]; Hlavac, Pavel ^[4]; Smerhovsky, Zdenek ^[3]; Naccarati, Alessio; Polakova, Veronika ^[1]; Soucek, Pavel ^[3]; Kuricova, Miroslava ^[5]; Manini, Paola ^[6]; Kumar, Rajiv; Hemminki, Kari ^[7]; Vodicka, Pavel ^[1]

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1. Institute of Experimental Medicine, Academy of Sciences of Czech Republic, Prague (Czech Republic)
2. Purkynje Military Medical Academy, Hradec Kralove (Czech Republic)
3. National Inst. Public Health, Prague (Czech Republic)
4. Dept. Occup. Medicine at Regional Hygiene Station, Hradec Kralove (Czech Republic)
5. Res. Base, Slovak Medical Univ., Bratislava (Slovakia)
6. Dept. of Clinical Medicine, Nephrology and Health Sciences, University of Parma (Italy)
7. German Cancer Research Center, Deutsches Krebs Forschungs Zentrum, DKFZ, Heidelberg (Germany)

Decreased levels of single-strand breaks in DNA (SSBs), reflecting DNA damage, have previously been observed with increased styrene exposure in contrast to a dose-dependent increase in the base-excision repair capacity. To clarify further the above aspects, we have investigated the associations between SSBs, micronuclei, DNA repair capacity and mRNA expression in XRCC1, hOGG1 and XPC genes on 71 styrene-exposed and 51 control individuals. Styrene concentrations at workplace and in blood characterized occupational exposure. The workers were divided into low (below 50 mg/m{sup 3}) and high (above 50 mg/m{sup 3}) styrene exposure groups. DNA damage and DNA repair capacity were analyzed in peripheral blood lymphocytes by Comet assay. The mRNA expression levels were determined by qPCR. A significant negative correlation was observed between SSBs and styrene concentration at workplace (R = - 0.38, p = 0.001); SSBs were also significantly higher in men (p = 0.001). The capacity to repair irradiation-induced DNA damage was the highest in the low exposure group (1.34 {+-} 1.00 SSB/10{sup 9} Da), followed by high exposure group (0.72 {+-} 0.81 SSB/10{sup 9} Da) and controls (0.65 {+-} 0.82 SSB/10{sup 9} Da). The mRNA expression levels of XRCC1, hOGG1 and XPC negatively correlated with styrene concentrations in blood and at workplace (p < 0.001) and positively with SSBs (p < 0.001). Micronuclei were not affected by styrene exposure, but were higher in older persons and in women (p < 0.001). In this study, we did not confirm previous findings on an increased DNA repair response to styrene-induced genotoxicity. However, negative correlations of SSBs and mRNA expression levels of XRCC1, hOGG1 and XPC with styrene exposure warrant further highly-targeted study.

• OSTI ID: 21451191
• Journal Information: Toxicology and Applied Pharmacology, Vol. 248, Issue 3; Other Information: DOI: 10.1016/j.taap.2010.07.027; PII: S0041-008X(10)00273-5; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
DNA REPAIR
GENES
MESSENGER-RNA
MODULATION
OCCUPATIONAL EXPOSURE
STRAND BREAKS
STYRENE
TOXICITY
ALKYLATED AROMATICS
AROMATICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA DAMAGES
HYDROCARBONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
REPAIR
RNA