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Title: Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome

Abstract

To investigate the possible involvement of betel-quid chewing in adipocyte dysfunction, we determined the effects of arecoline, a major alkaloid in areca nuts, on adipogenic differentiation (adipogenesis), lipolysis, and glucose uptake by fat cells. Using mouse 3T3-L1 preadipocytes, we showed that arecoline inhibited adipogenesis as determined by oil droplet formation and adipogenic marker gene expression. The effects of arecoline on lipolysis of differentiated 3T3-L1 adipocytes were determined by the glycerol release assay, indicating that arecoline induced lipolysis in an adenylyl cyclase-dependent manner. The diabetogenic effects of arecoline on differentiated 3T3-L1 adipocytes were evaluated by the glucose uptake assay, revealing that {>=} 300 {mu}M arecoline significantly attenuated insulin-induced glucose uptake; however, no marked effect on basal glucose uptake was detected. Moreover, using 94 subjects that were randomly selected from a health check-up, we determined the association of betel-quid chewing with hyperlipidemia and its related risk factors. Hyperlipidemia frequency and serum triglyceride levels of betel-quid chewers were significantly higher than those of non-betel-quid chewers. In this study, we demonstrated that arecoline inhibits adipogenic differentiation, induces adenylyl cyclase-dependent lipolysis, and interferes with insulin-induced glucose uptake. Arecoline-induced fat cell dysfunction may lead to hyperlipidemia and hyperglycemia/insulin-resistance. These findings provide the first in vitro evidencemore » of betel-quid chewing modulation of adipose cell metabolism that could contribute to the explanation of the association of this habit with metabolic syndrome disorders.« less

Authors:
 [1];  [1];  [2];  [3]; ; ;  [1];  [1]
  1. Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan (China)
  2. Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan (China)
  3. Department of Radiology, Pingtung Christian Hospital, Pingtung, Taiwan (China)
Publication Date:
OSTI Identifier:
21451170
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 245; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2010.04.008; PII: S0041-008X(10)00135-3; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; 60 APPLIED LIFE SCIENCES; ALKALOIDS; FAT CELLS; GLUCOSE; HYPERGLYCEMIA; INSULIN; METABOLISM; MICE; TRIGLYCERIDES; UPTAKE; ALDEHYDES; ANIMAL CELLS; ANIMALS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; ESTERS; HEXOSES; HORMONES; LIPIDS; MAMMALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PROTEINS; RODENTS; SACCHARIDES; SOMATIC CELLS; VERTEBRATES

Citation Formats

Hsu, Hsin-Fen, Tsou, Tsui-Chun, Chao, How-Ran, Shy, Cherng-Gueih, Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan, Kuo, Ya-Ting, Tsai, Feng-Yuan, Yeh, Szu-Ching, Ko, Ying-Chin, and Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome. United States: N. p., 2010. Web. doi:10.1016/j.taap.2010.04.008.
Hsu, Hsin-Fen, Tsou, Tsui-Chun, Chao, How-Ran, Shy, Cherng-Gueih, Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan, Kuo, Ya-Ting, Tsai, Feng-Yuan, Yeh, Szu-Ching, Ko, Ying-Chin, & Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome. United States. https://doi.org/10.1016/j.taap.2010.04.008
Hsu, Hsin-Fen, Tsou, Tsui-Chun, Chao, How-Ran, Shy, Cherng-Gueih, Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan, Kuo, Ya-Ting, Tsai, Feng-Yuan, Yeh, Szu-Ching, Ko, Ying-Chin, and Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. 2010. "Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome". United States. https://doi.org/10.1016/j.taap.2010.04.008.
@article{osti_21451170,
title = {Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome},
author = {Hsu, Hsin-Fen and Tsou, Tsui-Chun and Chao, How-Ran and Shy, Cherng-Gueih and Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan and Kuo, Ya-Ting and Tsai, Feng-Yuan and Yeh, Szu-Ching and Ko, Ying-Chin and Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan},
abstractNote = {To investigate the possible involvement of betel-quid chewing in adipocyte dysfunction, we determined the effects of arecoline, a major alkaloid in areca nuts, on adipogenic differentiation (adipogenesis), lipolysis, and glucose uptake by fat cells. Using mouse 3T3-L1 preadipocytes, we showed that arecoline inhibited adipogenesis as determined by oil droplet formation and adipogenic marker gene expression. The effects of arecoline on lipolysis of differentiated 3T3-L1 adipocytes were determined by the glycerol release assay, indicating that arecoline induced lipolysis in an adenylyl cyclase-dependent manner. The diabetogenic effects of arecoline on differentiated 3T3-L1 adipocytes were evaluated by the glucose uptake assay, revealing that {>=} 300 {mu}M arecoline significantly attenuated insulin-induced glucose uptake; however, no marked effect on basal glucose uptake was detected. Moreover, using 94 subjects that were randomly selected from a health check-up, we determined the association of betel-quid chewing with hyperlipidemia and its related risk factors. Hyperlipidemia frequency and serum triglyceride levels of betel-quid chewers were significantly higher than those of non-betel-quid chewers. In this study, we demonstrated that arecoline inhibits adipogenic differentiation, induces adenylyl cyclase-dependent lipolysis, and interferes with insulin-induced glucose uptake. Arecoline-induced fat cell dysfunction may lead to hyperlipidemia and hyperglycemia/insulin-resistance. These findings provide the first in vitro evidence of betel-quid chewing modulation of adipose cell metabolism that could contribute to the explanation of the association of this habit with metabolic syndrome disorders.},
doi = {10.1016/j.taap.2010.04.008},
url = {https://www.osti.gov/biblio/21451170}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 245,
place = {United States},
year = {Tue Jun 15 00:00:00 EDT 2010},
month = {Tue Jun 15 00:00:00 EDT 2010}
}