Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome
Abstract
To investigate the possible involvement of betel-quid chewing in adipocyte dysfunction, we determined the effects of arecoline, a major alkaloid in areca nuts, on adipogenic differentiation (adipogenesis), lipolysis, and glucose uptake by fat cells. Using mouse 3T3-L1 preadipocytes, we showed that arecoline inhibited adipogenesis as determined by oil droplet formation and adipogenic marker gene expression. The effects of arecoline on lipolysis of differentiated 3T3-L1 adipocytes were determined by the glycerol release assay, indicating that arecoline induced lipolysis in an adenylyl cyclase-dependent manner. The diabetogenic effects of arecoline on differentiated 3T3-L1 adipocytes were evaluated by the glucose uptake assay, revealing that {>=} 300 {mu}M arecoline significantly attenuated insulin-induced glucose uptake; however, no marked effect on basal glucose uptake was detected. Moreover, using 94 subjects that were randomly selected from a health check-up, we determined the association of betel-quid chewing with hyperlipidemia and its related risk factors. Hyperlipidemia frequency and serum triglyceride levels of betel-quid chewers were significantly higher than those of non-betel-quid chewers. In this study, we demonstrated that arecoline inhibits adipogenic differentiation, induces adenylyl cyclase-dependent lipolysis, and interferes with insulin-induced glucose uptake. Arecoline-induced fat cell dysfunction may lead to hyperlipidemia and hyperglycemia/insulin-resistance. These findings provide the first in vitro evidencemore »
- Authors:
-
- Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan (China)
- Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan (China)
- Department of Radiology, Pingtung Christian Hospital, Pingtung, Taiwan (China)
- Publication Date:
- OSTI Identifier:
- 21451170
- Resource Type:
- Journal Article
- Journal Name:
- Toxicology and Applied Pharmacology
- Additional Journal Information:
- Journal Volume: 245; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2010.04.008; PII: S0041-008X(10)00135-3; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; 60 APPLIED LIFE SCIENCES; ALKALOIDS; FAT CELLS; GLUCOSE; HYPERGLYCEMIA; INSULIN; METABOLISM; MICE; TRIGLYCERIDES; UPTAKE; ALDEHYDES; ANIMAL CELLS; ANIMALS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; ESTERS; HEXOSES; HORMONES; LIPIDS; MAMMALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PROTEINS; RODENTS; SACCHARIDES; SOMATIC CELLS; VERTEBRATES
Citation Formats
Hsu, Hsin-Fen, Tsou, Tsui-Chun, Chao, How-Ran, Shy, Cherng-Gueih, Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan, Kuo, Ya-Ting, Tsai, Feng-Yuan, Yeh, Szu-Ching, Ko, Ying-Chin, and Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome. United States: N. p., 2010.
Web. doi:10.1016/j.taap.2010.04.008.
Hsu, Hsin-Fen, Tsou, Tsui-Chun, Chao, How-Ran, Shy, Cherng-Gueih, Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan, Kuo, Ya-Ting, Tsai, Feng-Yuan, Yeh, Szu-Ching, Ko, Ying-Chin, & Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome. United States. https://doi.org/10.1016/j.taap.2010.04.008
Hsu, Hsin-Fen, Tsou, Tsui-Chun, Chao, How-Ran, Shy, Cherng-Gueih, Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan, Kuo, Ya-Ting, Tsai, Feng-Yuan, Yeh, Szu-Ching, Ko, Ying-Chin, and Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. 2010.
"Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome". United States. https://doi.org/10.1016/j.taap.2010.04.008.
@article{osti_21451170,
title = {Effects of arecoline on adipogenesis, lipolysis, and glucose uptake of adipocytes-A possible role of betel-quid chewing in metabolic syndrome},
author = {Hsu, Hsin-Fen and Tsou, Tsui-Chun and Chao, How-Ran and Shy, Cherng-Gueih and Graduate Institute of Pharmaceutical Science, Department of Pharmacy, Tajen University, Yan-Pu, Pingtung, Taiwan and Kuo, Ya-Ting and Tsai, Feng-Yuan and Yeh, Szu-Ching and Ko, Ying-Chin and Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan},
abstractNote = {To investigate the possible involvement of betel-quid chewing in adipocyte dysfunction, we determined the effects of arecoline, a major alkaloid in areca nuts, on adipogenic differentiation (adipogenesis), lipolysis, and glucose uptake by fat cells. Using mouse 3T3-L1 preadipocytes, we showed that arecoline inhibited adipogenesis as determined by oil droplet formation and adipogenic marker gene expression. The effects of arecoline on lipolysis of differentiated 3T3-L1 adipocytes were determined by the glycerol release assay, indicating that arecoline induced lipolysis in an adenylyl cyclase-dependent manner. The diabetogenic effects of arecoline on differentiated 3T3-L1 adipocytes were evaluated by the glucose uptake assay, revealing that {>=} 300 {mu}M arecoline significantly attenuated insulin-induced glucose uptake; however, no marked effect on basal glucose uptake was detected. Moreover, using 94 subjects that were randomly selected from a health check-up, we determined the association of betel-quid chewing with hyperlipidemia and its related risk factors. Hyperlipidemia frequency and serum triglyceride levels of betel-quid chewers were significantly higher than those of non-betel-quid chewers. In this study, we demonstrated that arecoline inhibits adipogenic differentiation, induces adenylyl cyclase-dependent lipolysis, and interferes with insulin-induced glucose uptake. Arecoline-induced fat cell dysfunction may lead to hyperlipidemia and hyperglycemia/insulin-resistance. These findings provide the first in vitro evidence of betel-quid chewing modulation of adipose cell metabolism that could contribute to the explanation of the association of this habit with metabolic syndrome disorders.},
doi = {10.1016/j.taap.2010.04.008},
url = {https://www.osti.gov/biblio/21451170},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 245,
place = {United States},
year = {Tue Jun 15 00:00:00 EDT 2010},
month = {Tue Jun 15 00:00:00 EDT 2010}
}