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Title: Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice

Abstract

Purpose: Radiation-induced intestinal injury is a significant clinical problem in patients undergoing abdominal radiotherapy (RT). Berberine has been used as an antimicrobial, anti-inflammatory, and antimotility agent. The present study investigated the protective effect of berberine against radiation-induced intestinal injury. Methods and Materials: The mice were administrated berberine or distilled water. A total of 144 mice underwent 0, 3, 6, 12, or 16 Gy single session whole-abdominal RT and 16 mice underwent 3 Gy/fraction/d for four fractions of fractionated abdominal RT. Tumor necrosis factor-{alpha}, interleukin-10, diamine oxidase, intestinal fatty acid-binding protein, malonaldehyde, and apoptosis were assayed in the mice after RT. The body weight and food intake of the mice receiving fractionated RT were recorded. Another 72 mice who had undergone 12, 16, or 20 Gy abdominal RT were monitored for mortality every 12 h. Results: The body weight and food intake of the mice administered with distilled water decreased significantly compared with before RT. After the same dose of abdominal RT, tumor necrosis factor-{alpha}, diamine oxidase, intestinal fatty acid-binding protein in plasma and malonalhehyde and apoptosis of the intestine were significantly greater in the control group than in the mice administered berberine (p < .05-.01). In contrast, interleukin-10 in themore » mice with berberine treatment was significantly greater than in the control group (p < .01). A similar result was found in the fractionated RT experiment and at different points after 16 Gy abdominal RT (p < .05-.01). Berberine treatment significantly delayed the point of death after 20 Gy, but not 16 Gy, abdominal RT (p < .01). Conclusion: Treatment with berberine can delay mortality and attenuated intestinal injury in mice undergoing whole abdominal RT. These findings could provide a useful therapeutic strategy for radiation-induced intestinal injury.« less

Authors:
 [1];  [2];  [3]; ;  [1];  [4]; ;  [1];  [1]
  1. Institute for Cancer Research in People's Liberation Army, Xinqiao Hospital, Third Military Medical University, Chongqing (China)
  2. Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing (China)
  3. Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing (China)
  4. Department of Plastic Surgery, Southwest Hospital, Third Military Medical University, Chongqing (China)
Publication Date:
OSTI Identifier:
21436127
Resource Type:
Journal Article
Journal Name:
International Journal of Radiation Oncology, Biology and Physics
Additional Journal Information:
Journal Volume: 77; Journal Issue: 5; Other Information: DOI: 10.1016/j.ijrobp.2010.02.062; PII: S0360-3016(10)00451-7; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0360-3016
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; INTESTINES; MICE; RADIATION INJURIES; RADIOTHERAPY; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BODY; DIGESTIVE SYSTEM; DISEASES; GASTROINTESTINAL TRACT; INJURIES; MAMMALS; MEDICINE; NUCLEAR MEDICINE; ORGANS; RADIATION EFFECTS; RADIOLOGY; RODENTS; THERAPY; VERTEBRATES

Citation Formats

Li Guanghui, Zhang Yaping, Tang Jinliang, Chen Zhengtang, Hu Yide, Wei Hong, Li Dezhi, Hao Ping, and Wang Donglin, E-mail: wdl_tmmu@yahoo.c. Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice. United States: N. p., 2010. Web.
Li Guanghui, Zhang Yaping, Tang Jinliang, Chen Zhengtang, Hu Yide, Wei Hong, Li Dezhi, Hao Ping, & Wang Donglin, E-mail: wdl_tmmu@yahoo.c. Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice. United States.
Li Guanghui, Zhang Yaping, Tang Jinliang, Chen Zhengtang, Hu Yide, Wei Hong, Li Dezhi, Hao Ping, and Wang Donglin, E-mail: wdl_tmmu@yahoo.c. Sun . "Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice". United States.
@article{osti_21436127,
title = {Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice},
author = {Li Guanghui and Zhang Yaping and Tang Jinliang and Chen Zhengtang and Hu Yide and Wei Hong and Li Dezhi and Hao Ping and Wang Donglin, E-mail: wdl_tmmu@yahoo.c},
abstractNote = {Purpose: Radiation-induced intestinal injury is a significant clinical problem in patients undergoing abdominal radiotherapy (RT). Berberine has been used as an antimicrobial, anti-inflammatory, and antimotility agent. The present study investigated the protective effect of berberine against radiation-induced intestinal injury. Methods and Materials: The mice were administrated berberine or distilled water. A total of 144 mice underwent 0, 3, 6, 12, or 16 Gy single session whole-abdominal RT and 16 mice underwent 3 Gy/fraction/d for four fractions of fractionated abdominal RT. Tumor necrosis factor-{alpha}, interleukin-10, diamine oxidase, intestinal fatty acid-binding protein, malonaldehyde, and apoptosis were assayed in the mice after RT. The body weight and food intake of the mice receiving fractionated RT were recorded. Another 72 mice who had undergone 12, 16, or 20 Gy abdominal RT were monitored for mortality every 12 h. Results: The body weight and food intake of the mice administered with distilled water decreased significantly compared with before RT. After the same dose of abdominal RT, tumor necrosis factor-{alpha}, diamine oxidase, intestinal fatty acid-binding protein in plasma and malonalhehyde and apoptosis of the intestine were significantly greater in the control group than in the mice administered berberine (p < .05-.01). In contrast, interleukin-10 in the mice with berberine treatment was significantly greater than in the control group (p < .01). A similar result was found in the fractionated RT experiment and at different points after 16 Gy abdominal RT (p < .05-.01). Berberine treatment significantly delayed the point of death after 20 Gy, but not 16 Gy, abdominal RT (p < .01). Conclusion: Treatment with berberine can delay mortality and attenuated intestinal injury in mice undergoing whole abdominal RT. These findings could provide a useful therapeutic strategy for radiation-induced intestinal injury.},
doi = {},
journal = {International Journal of Radiation Oncology, Biology and Physics},
issn = {0360-3016},
number = 5,
volume = 77,
place = {United States},
year = {2010},
month = {8}
}