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Title: Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine

Abstract

We present a femtosecond Laser Two-Photon Polymerization (LTPP) system of large scale three-dimensional structuring for applications in tissue engineering. The direct laser writing system enables fabrication of artificial polymeric scaffolds over a large area (up to cm in lateral size) with sub-micrometer resolution which could find practical applications in biomedicine and surgery. Yb:KGW femtosecond laser oscillator (Pharos, Light Conversion. Co. Ltd.) is used as an irradiation source (75 fs, 515 nm (frequency doubled), 80 MHz). The sample is mounted on wide range linear motor driven stages having 10 nm sample positioning resolution (XY--ALS130-100, Z--ALS130-50, Aerotech, Inc.). These stages guarantee an overall travelling range of 100 mm into X and Y directions and 50 mm in Z direction and support the linear scanning speed up to 300 mm/s. By moving the sample three-dimensionally the position of laser focus in the photopolymer is changed and one is able to write complex 3D (three-dimensional) structures. An illumination system and CMOS camera enables online process monitoring. Control of all equipment is automated via custom made computer software ''3D-Poli'' specially designed for LTPP applications. Structures can be imported from computer aided design STereoLihography (stl) files or programmed directly. It can be used for rapid LTPPmore » structuring in various photopolymers (SZ2080, AKRE19, PEG-DA-258) which are known to be suitable for bio-applications. Microstructured scaffolds can be produced on different substrates like glass, plastic and metal. In this paper, we present microfabricated polymeric scaffolds over a large area and growing of adult rabbit myogenic stem cells on them. Obtained results show the polymeric scaffolds to be applicable for cell growth practice. It exhibit potential to use it for artificial pericardium in the experimental model in the future.« less

Authors:
; ; ; ; ; ; ; ;  [1]; ; ;  [2];  [3];  [4];  [5]
  1. Vilnius University, Physics Faculty, Department of Quantum Electronics, Laser Research Center, Sauletekio ave. 10, LT-10223 Vilnius (Lithuania)
  2. Institute of Biochemistry, Vivarium, Mokslininkuo str. 12, LT-08662 Vilnius (Lithuania)
  3. Vilnius University Hospital Santariskiuo Klinikos, Santariskiuo g. 2, LT-08661 Vilnius (Lithuania)
  4. Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas, Vassilika Vouton, 711 10 Heraklion, Crete (Greece)
  5. Vilnius University, Faculty of Medicine, Heart Surgery Center, Santariskiuo 2, LT-08661, Vilnius (Lithuania)
Publication Date:
OSTI Identifier:
21428715
Resource Type:
Journal Article
Journal Name:
AIP Conference Proceedings
Additional Journal Information:
Journal Volume: 1288; Journal Issue: 1; Conference: International Commission for Optics topical meeting on emerging trends and novel materials in photonics, Delphi (Greece), 7-9 Oct 2010; Other Information: DOI: 10.1063/1.3521344; (c) 2010 American Institute of Physics; Journal ID: ISSN 0094-243X
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; CAMERAS; COMPUTER CODES; COMPUTER-AIDED DESIGN; FABRICATION; HARMONIC OSCILLATORS; LIGHTING SYSTEMS; MHZ RANGE; MULTI-PHOTON PROCESSES; OSCILLATORS; PERICARDIUM; PHOTONS; PLASTICS; POLYMERIZATION; POSITIONING; PULSED IRRADIATION; RESOLUTION; STEM CELLS; SUBSTRATES; SURGERY; ANIMAL CELLS; BODY; BOSONS; CARDIOVASCULAR SYSTEM; CHEMICAL REACTIONS; DESIGN; ELECTRONIC EQUIPMENT; ELEMENTARY PARTICLES; ENERGY SYSTEMS; EQUIPMENT; FREQUENCY RANGE; HEART; IRRADIATION; MASSLESS PARTICLES; MATERIALS; MEDICINE; MEMBRANES; ORGANIC COMPOUNDS; ORGANIC POLYMERS; ORGANS; PETROCHEMICALS; PETROLEUM PRODUCTS; POLYMERS; SEROUS MEMBRANES; SOMATIC CELLS; SYNTHETIC MATERIALS

Citation Formats

Malinauskas, M, Purlys, V, Zukauskas, A, Rutkauskas, M, Danilevicius, P, Paipulas, D, Bickauskaite, G, Gadonas, R, Piskarskas, A, Bukelskis, L, Baltriukiene, D, Bukelskiene, V, Sirmenis, R, Gaidukeviciute, A, and Sirvydis, V. Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine. United States: N. p., 2010. Web. doi:10.1063/1.3521344.
Malinauskas, M, Purlys, V, Zukauskas, A, Rutkauskas, M, Danilevicius, P, Paipulas, D, Bickauskaite, G, Gadonas, R, Piskarskas, A, Bukelskis, L, Baltriukiene, D, Bukelskiene, V, Sirmenis, R, Gaidukeviciute, A, & Sirvydis, V. Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine. United States. doi:10.1063/1.3521344.
Malinauskas, M, Purlys, V, Zukauskas, A, Rutkauskas, M, Danilevicius, P, Paipulas, D, Bickauskaite, G, Gadonas, R, Piskarskas, A, Bukelskis, L, Baltriukiene, D, Bukelskiene, V, Sirmenis, R, Gaidukeviciute, A, and Sirvydis, V. Wed . "Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine". United States. doi:10.1063/1.3521344.
@article{osti_21428715,
title = {Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine},
author = {Malinauskas, M and Purlys, V and Zukauskas, A and Rutkauskas, M and Danilevicius, P and Paipulas, D and Bickauskaite, G and Gadonas, R and Piskarskas, A and Bukelskis, L and Baltriukiene, D and Bukelskiene, V and Sirmenis, R and Gaidukeviciute, A and Sirvydis, V},
abstractNote = {We present a femtosecond Laser Two-Photon Polymerization (LTPP) system of large scale three-dimensional structuring for applications in tissue engineering. The direct laser writing system enables fabrication of artificial polymeric scaffolds over a large area (up to cm in lateral size) with sub-micrometer resolution which could find practical applications in biomedicine and surgery. Yb:KGW femtosecond laser oscillator (Pharos, Light Conversion. Co. Ltd.) is used as an irradiation source (75 fs, 515 nm (frequency doubled), 80 MHz). The sample is mounted on wide range linear motor driven stages having 10 nm sample positioning resolution (XY--ALS130-100, Z--ALS130-50, Aerotech, Inc.). These stages guarantee an overall travelling range of 100 mm into X and Y directions and 50 mm in Z direction and support the linear scanning speed up to 300 mm/s. By moving the sample three-dimensionally the position of laser focus in the photopolymer is changed and one is able to write complex 3D (three-dimensional) structures. An illumination system and CMOS camera enables online process monitoring. Control of all equipment is automated via custom made computer software ''3D-Poli'' specially designed for LTPP applications. Structures can be imported from computer aided design STereoLihography (stl) files or programmed directly. It can be used for rapid LTPP structuring in various photopolymers (SZ2080, AKRE19, PEG-DA-258) which are known to be suitable for bio-applications. Microstructured scaffolds can be produced on different substrates like glass, plastic and metal. In this paper, we present microfabricated polymeric scaffolds over a large area and growing of adult rabbit myogenic stem cells on them. Obtained results show the polymeric scaffolds to be applicable for cell growth practice. It exhibit potential to use it for artificial pericardium in the experimental model in the future.},
doi = {10.1063/1.3521344},
journal = {AIP Conference Proceedings},
issn = {0094-243X},
number = 1,
volume = 1288,
place = {United States},
year = {2010},
month = {11}
}