Enhanced human papillomavirus type 8 oncogene expression levels are crucial for skin tumorigenesis in transgenic mice

Journal Article · Sun Aug 01 00:00:00 EDT 2010 · Virology

DOI:https://doi.org/10.1016/j.virol.2010.04.013· OSTI ID:21416178

Hufbauer, M ^[1]; Lazic, D; Akguel, B ^[1]; Brandsma, J L ^[2]; Pfister, H; Weissenborn, S J ^[1]

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1. Institute of Virology, University of Cologne, Cologne (Germany)
2. Section of Comparative Medicine and Department of Pathology, Yale University School of Medicine, New Haven, CT (United States)

Human papillomavirus 8 (HPV8) is involved in skin cancer development in epidermodysplasia verruciformis patients. Transgenic mice expressing HPV8 early genes (HPV8-CER) developed papillomas, dysplasias and squamous cell carcinomas. UVA/B-irradiation and mechanical wounding of HPV8-CER mouse skin led to prompt papilloma induction in about 3 weeks. The aim of this study was to analyze the kinetics and level of transgene expression in response to skin irritations. Transgene expression was already enhanced 1 to 2 days after UVA/B-irradiation or tape-stripping and maintained during papilloma development. The enhanced transgene expression could be assigned to UVB and not to UVA. Papilloma development was thus always paralleled by an increased transgene expression irrespective of the type of skin irritation. A knock-down of E6 mRNA by tattooing HPV8-E6-specific siRNA led to a delay and a lower incidence of papilloma development. This indicates that the early increase of viral oncogene expression is crucial for induction of papillomatosis.

• OSTI ID: 21416178
• Journal Information: Virology, Vol. 403, Issue 2; Other Information: DOI: 10.1016/j.virol.2010.04.013; PII: S0042-6822(10)00263-1; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
• Country of Publication: United States
• Language: English

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