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Title: Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat

Abstract

This report describes the discovery and characterization of a new fusogenic orthoreovirus, Broome virus (BroV), isolated from a little red flying-fox (Pteropus scapulatus). The BroV genome consists of 10 dsRNA segments, each having a 3' terminal pentanucleotide sequence conserved amongst all members of the genus Orthoreovirus, and a unique 5' terminal pentanucleotide sequence. The smallest genome segment is bicistronic and encodes two small nonstructural proteins, one of which is a novel fusion associated small transmembrane (FAST) protein responsible for syncytium formation, but no cell attachment protein. The low amino acid sequence identity between BroV proteins and those of other orthoreoviruses (13-50%), combined with phylogenetic analyses of structural and nonstructural proteins provide evidence to support the classification of BroV in a new sixth species group within the genus Orthoreovirus.

Authors:
 [1];  [1];  [1]; ; ; ; ;  [1]
  1. CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria (Australia)
Publication Date:
OSTI Identifier:
21416175
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 402; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2009.11.048; PII: S0042-6822(09)00784-3; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; BATS; FRUITS; PROTEINS; VIRUSES; ANIMALS; FOOD; MAMMALS; MICROORGANISMS; MOLECULAR STRUCTURE; ORGANIC COMPOUNDS; PARASITES; VERTEBRATES

Citation Formats

Thalmann, Claudia M, Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland, Cummins, David Michael, Meng, Yu, Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Lunt, Ross, Pritchard, Lindsay Ian, Hansson, Eric, Crameri, Sandra, Hyatt, Alex, Wang Linfa, E-mail: Linfa.Wang@csiro.a, and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland. Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat. United States: N. p., 2010. Web. doi:10.1016/j.virol.2009.11.048.
Thalmann, Claudia M, Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland, Cummins, David Michael, Meng, Yu, Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Lunt, Ross, Pritchard, Lindsay Ian, Hansson, Eric, Crameri, Sandra, Hyatt, Alex, Wang Linfa, E-mail: Linfa.Wang@csiro.a, & Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland. Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat. United States. doi:10.1016/j.virol.2009.11.048.
Thalmann, Claudia M, Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland, Cummins, David Michael, Meng, Yu, Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland, Lunt, Ross, Pritchard, Lindsay Ian, Hansson, Eric, Crameri, Sandra, Hyatt, Alex, Wang Linfa, E-mail: Linfa.Wang@csiro.a, and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland. Sun . "Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat". United States. doi:10.1016/j.virol.2009.11.048.
@article{osti_21416175,
title = {Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat},
author = {Thalmann, Claudia M and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland and School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland and Cummins, David Michael and Meng, Yu and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland and Lunt, Ross and Pritchard, Lindsay Ian and Hansson, Eric and Crameri, Sandra and Hyatt, Alex and Wang Linfa, E-mail: Linfa.Wang@csiro.a and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease, Brisbane, Queensland},
abstractNote = {This report describes the discovery and characterization of a new fusogenic orthoreovirus, Broome virus (BroV), isolated from a little red flying-fox (Pteropus scapulatus). The BroV genome consists of 10 dsRNA segments, each having a 3' terminal pentanucleotide sequence conserved amongst all members of the genus Orthoreovirus, and a unique 5' terminal pentanucleotide sequence. The smallest genome segment is bicistronic and encodes two small nonstructural proteins, one of which is a novel fusion associated small transmembrane (FAST) protein responsible for syncytium formation, but no cell attachment protein. The low amino acid sequence identity between BroV proteins and those of other orthoreoviruses (13-50%), combined with phylogenetic analyses of structural and nonstructural proteins provide evidence to support the classification of BroV in a new sixth species group within the genus Orthoreovirus.},
doi = {10.1016/j.virol.2009.11.048},
journal = {Virology},
issn = {0042-6822},
number = 1,
volume = 402,
place = {United States},
year = {2010},
month = {6}
}