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Title: Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns

Abstract

We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.

Authors:
;  [1]; ;  [2]; ; ;  [3];  [4]; ; ; ; ;  [5];  [6]; ; ;  [7];  [8];  [3]
  1. Laboratory of Atomic and Solid State Physics Cornell University, Ithaca, New York 14853-2501 (United States)
  2. SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California, 94025 (United States)
  3. Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607 (Germany)
  4. Photon Science, DESY, Notkestrasse 85, Hamburg 22607 (Germany)
  5. Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3, Box 596, SE-75124 Uppsala (Sweden)
  6. Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley California 94720 (United States)
  7. Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg (Germany)
  8. Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550 (United States)
Publication Date:
OSTI Identifier:
21415247
Resource Type:
Journal Article
Journal Name:
Physical Review Letters
Additional Journal Information:
Journal Volume: 104; Journal Issue: 22; Other Information: DOI: 10.1103/PhysRevLett.104.225501; (c) 2010 The American Physical Society; Journal ID: ISSN 0031-9007
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; DESY; FLUCTUATIONS; FOURIER ANALYSIS; NANOSTRUCTURES; ORIENTATION; PHOTONS; RANDOMNESS; THREE-DIMENSIONAL CALCULATIONS; TOMOGRAPHY; X RADIATION; ACCELERATORS; BOSONS; CYCLIC ACCELERATORS; DIAGNOSTIC TECHNIQUES; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; IONIZING RADIATIONS; MASSLESS PARTICLES; RADIATIONS; SYNCHROTRONS; VARIATIONS

Citation Formats

Loh, N D, Elser, V, Bogan, M J, Boutet, S, Barty, A, Schulz, J, Liang, M, Bajt, S, Hajdu, J, Ekeberg, T, Maia, F. R. N. C., Seibert, M M, Iwan, B, Timneanu, N, Marchesini, S, Schlichting, I, Shoeman, R L, Lomb, L, Max Planck Advanced Study Group, Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Frank, M, Chapman, H N, and University of Hamburg, Luruper Chaussee 149, Hamburg 22761. Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns. United States: N. p., 2010. Web. doi:10.1103/PHYSREVLETT.104.225501.
Loh, N D, Elser, V, Bogan, M J, Boutet, S, Barty, A, Schulz, J, Liang, M, Bajt, S, Hajdu, J, Ekeberg, T, Maia, F. R. N. C., Seibert, M M, Iwan, B, Timneanu, N, Marchesini, S, Schlichting, I, Shoeman, R L, Lomb, L, Max Planck Advanced Study Group, Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Frank, M, Chapman, H N, & University of Hamburg, Luruper Chaussee 149, Hamburg 22761. Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns. United States. doi:10.1103/PHYSREVLETT.104.225501.
Loh, N D, Elser, V, Bogan, M J, Boutet, S, Barty, A, Schulz, J, Liang, M, Bajt, S, Hajdu, J, Ekeberg, T, Maia, F. R. N. C., Seibert, M M, Iwan, B, Timneanu, N, Marchesini, S, Schlichting, I, Shoeman, R L, Lomb, L, Max Planck Advanced Study Group, Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Frank, M, Chapman, H N, and University of Hamburg, Luruper Chaussee 149, Hamburg 22761. Fri . "Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns". United States. doi:10.1103/PHYSREVLETT.104.225501.
@article{osti_21415247,
title = {Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns},
author = {Loh, N D and Elser, V and Bogan, M J and Boutet, S and Barty, A and Schulz, J and Liang, M and Bajt, S and Hajdu, J and Ekeberg, T and Maia, F. R. N. C. and Seibert, M M and Iwan, B and Timneanu, N and Marchesini, S and Schlichting, I and Shoeman, R L and Lomb, L and Max Planck Advanced Study Group, Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607 and Frank, M and Chapman, H N and University of Hamburg, Luruper Chaussee 149, Hamburg 22761},
abstractNote = {We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.},
doi = {10.1103/PHYSREVLETT.104.225501},
journal = {Physical Review Letters},
issn = {0031-9007},
number = 22,
volume = 104,
place = {United States},
year = {2010},
month = {6}
}