Estimation of Anthocyanin Content of Berries by NIR Method
- Agricultural Academy of Bulgaria, Canning Research Institute, Plovdiv, 154 Blvd. Vasil Aprilov 4000 Plovdiv (Bulgaria)
Anthocyanin contents of fruits were estimated by VIS spectrophotometer and compared with spectra measured by NIR spectrophotometer (600-1100 nm step 10 nm). The aim was to find a relationship between NIR method and traditional spectrophotometric method. The testing protocol, using NIR, is easier, faster and non-destructive. NIR spectra were prepared in pairs, reflectance and transmittance. A modular spectrocomputer, realized on the basis of a monochromator and peripherals Bentham Instruments Ltd (GB) and a photometric camera created at Canning Research Institute, were used. An important feature of this camera is the possibility offered for a simultaneous measurement of both transmittance and reflectance with geometry patterns T0/180 and R0/45. The collected spectra were analyzed by CAMO Unscrambler 9.1 software, with PCA, PLS, PCR methods. Based on the analyzed spectra quality and quantity sensitive calibrations were prepared. The results showed that the NIR method allows measuring of the total anthocyanin content in fresh berry fruits or processed products without destroying them.
- OSTI ID:
- 21371475
- Journal Information:
- AIP Conference Proceedings, Vol. 1203, Issue 1; Conference: 7. international conference of the Balkan Physical Union, Alexandroupolis (Greece), 9-13 Sep 2009; Other Information: DOI: 10.1063/1.3322508; (c) 2010 American Institute of Physics; ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ABSORPTION SPECTROSCOPY
BERRIES
CALIBRATION
CAMERAS
MONOCHROMATORS
NEAR INFRARED RADIATION
NONDESTRUCTIVE ANALYSIS
POLYMERASE CHAIN REACTION
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
SPECTRAL REFLECTANCE
SPECTROPHOTOMETRY
CHEMICAL ANALYSIS
ELECTROMAGNETIC RADIATION
FOOD
FRUITS
GENE AMPLIFICATION
INFRARED RADIATION
OPTICAL PROPERTIES
ORGANIC COMPOUNDS
PHYSICAL PROPERTIES
RADIATIONS
SPECTROSCOPY